CSP antibody - 154 004

CSP is a chaperon at the synapse

Guinea pig polyclonal antiserum

Cat. No.: 154 004

Amount: 100 µl

Price: $365.00

Cat. No. 154 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 182 to 198 from rat CSP (UniProt Id: P60905)
Reactivity	Reacts with: rat, mouse. Other species not tested yet.
Data sheet	154_004.pdf

Cat. No.: 154 004

Amount: 100 µl

Price: $365.00

ICC

IHC

Background

Cysteine String Proteins CSPs are composed of an N-terminal J-domain and a central palmitoylated cysteine string. This post-translational modification shifts the molecular weight of CSP 1 in brain from 23 kDa to 34 kDa and confers membrane targeting of the protein.
CSP has been initially identified as a synaptic vesicle protein which is involved in Ca²⁺ triggered neurotransmitter release. Later CSP was also found on Large Dense Core Vesicles (LDCVs) of pancreatic insulin secretory β-cells, chromaffin cells and adipocytes. It has been shown to interact with SNARE proteins like VAMP 2, VAMP 7 and syntaxin 4.

1 ) Smith GB et al., J. Biol. Chem. (2005)
2 ) Evans GJ et al., Eur. J. Neurosci. (2005)
3 ) Miller LC et al., J. Biol. Chem. (2003)
4 ) Chamberlain LH et al., J. Cell. Sci. (2001)
5 ) Chamberlain LH et al., Biochem. J. (1998)
6 ) Chamberlain LH et al., J. Biol. Chem. (1997)
7 ) Chamberlain LH et al., J. Biol. Chem. (1996)

Protocols

Standard protocol

ICC

IHC

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