Synaptic Systems

Cat. No.: 151 003

Amount: 50 µg

Price: $455.00

Cat. No. 151 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 100 up to 1 : 1000 gallery IP: not tested yet ICC: 1 : 100 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 662 to 769 from rat GRIP (UniProt Id: P97879)
Reactivity	Reacts with: human (Q9Y3R0), rat (P97879), mouse (Q925T6), hamster. Other species not tested yet.
Specificity	Recognizes GRIP 1 and GRIP 2.
Matching control protein/peptide	151-0P
Data sheet	151_003.pdf

References for GRIP - 151 003

Dysregulation of AMPA Receptor Trafficking and Intracellular Vesicular Sorting in the Prefrontal Cortex of Dopamine Transporter Knock-Out Rats.
Targa G, Mottarlini F, Rizzi B, Leo D, Caffino L, Fumagalli F
Biomolecules (2023) 133: . 151 003 WB; tested species: rat

Activity-Based Anorexia Dynamically Dysregulates the Glutamatergic Synapse in the Nucleus Accumbens of Female Adolescent Rats.
Mottarlini F, Bottan G, Tarenzi B, Colciago A, Fumagalli F, Caffino L
Nutrients (2020) 1212: . 151 003 WB; tested species: rat

Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting.
Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, Heukamp A, Rannio S, Martens H, Barth J, Burk K, et al.
Science (New York, N.Y.) (2018) : . 151 003 WB; tested species: rat

Cat. No.: 151 003

Amount: 50 µg

Price: $455.00

ICC

Background

The glutamate receptor interacting protein GRIP is a post-synaptic scaffolding protein consisting of seven PDZ domains. It has been shown to interact with a diverse array of proteins like several AMPA receptors, HAP1-A, liprin-α and LAR receptor protein tyrosine phosphatases (LAR-RPTPs).
GRIP is also involved in the synaptic targeting of AMPA receptors.

1 ) Stegmüller J et al., J. Biol. Chem. (2003)
2 ) Hirbec H et al., J. Biol. Chem. (2002)
3 ) Yu G et al., Brain Res. Dev. Brain Res. (2001)
4 ) Brückner K et al., Neuron (1999)
5 ) Dong H et al., Nature (1997)

Protocols

ICC

OTHER

Preabsorbtion

Other products for this target

GRIP antibody - 151 003

References for GRIP - 151 003