|Cat. No. 147 103||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (AP staining) gallery
IP: yes (see remarks)
ICC: not recommended
IHC: not recommended
IHC-P/FFPE: not tested yet
|Immunogen||Recombinant protein corresponding to AA 294 to 736 from rat Gephyrin (UniProt Id: Q03555)|
Reacts with: rat (Q03555), mouse (Q8BUV3).
Other species not tested yet.
|Specificity||Specific for gephyrin.|
|Matching control protein/peptide||147-1P|
IP: For most effective IP use the denaturing SDS based solubilization protocol. Consider that protein-protein interactions may be affected.
Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.