|Cat. No. 147 011C2||
100 µg purified IgG, lyophilized, fluorescence-labeled with Sulfo-Cyanine 2. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
ICC: 1 : 250 up to 1 : 500 gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Subtype||IgG1 (κ light chain)|
|Immunogen||Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)|
Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reacts with: human (Q9NQX3), mouse (Q8BUV3), rat (Q03555), pig, goldfish, zebrafish.
Other species not tested yet.
|Specificity||Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. (K.O. verified) K.O.|
Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.