Gephyrin antibody - 147 011C2 K.O.

Gephyrin is a scaffolding protein associated with inhibitory neurotransmitter receptors

Mouse monoclonal purified IgG

Cat. No.: 147 011C2

Amount: 100 µg

Price: $465.00

Cat. No. 147 011C2	100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 2. Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy. Cyanine 2: λ_ex 492 nm / λ_em 508 nm Sulfo-Cyanine 3: λ_ex 554 nm / λ_em 568 nm Sulfo-Cyanine 5: λ_ex 649 nm / λ_em 667 nm (Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy. Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: 1 : 250 up to 1 : 500 gallery IHC: not tested yet IHC-P: not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	Cyanine 2
Clone	mAb7a
Subtype	IgG1 (κ light chain)
Immunogen	Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop	AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity	Reacts with: human (Q9NQX3), mouse (Q8BUV3), rat (Q03555), pig, goldfish, zebrafish. Other species not tested yet.
Specificity	Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O. validated
Data sheet	147_011c2.pdf

References for Gephyrin - 147 011C2

Cortical somatostatin interneuron subtypes form cell-type-specific circuits.
Wu SJ, Sevier E, Dwivedi D, Saldi GA, Hairston A, Yu S, Abbott L, Choi DH, Sherer M, Qiu Y, Shinde A, et al.
Neuron (2023) 11117: 2675-2692.e9. 147 011C2 IHC; tested species: mouse

Cat. No.: 147 011C2

Amount: 100 µg

Price: $465.00

ICC

Background

Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.

1 ) Hermann A et al., Biochem. Biophys. Res. Commun. (2001)
2 ) Kirsch J et al., Brain Res. (1993)
3 ) Kirsch J et al., Eur. J. Neurosci. (1993)

Protocols

ICC

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