Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 147 011C2

Gephyrin antibody - 147 011C2 K.O.

Gephyrin is a scaffolding protein associated with inhibitory neurotransmitter receptors
Mouse monoclonal purified IgG
Cat. No.: 147 011C2
Amount: 100 µg
Price: $465.00
Cat. No. 147 011C2 100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 2.

Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

Cyanine 2: λex 492 nm / λem 508 nm
Sulfo-Cyanine 3: λex 554 nm / λem 568 nm
Sulfo-Cyanine 5: λex 649 nm / λem 667 nm

(Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy.
Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates.

 Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 250 up to 1 : 500 gallery  
IHC: not tested yet
IHC-P: not recommended
Label Cyanine 2
Clone mAb7a
Subtype IgG1 (κ light chain)
Immunogen Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: human (Q9NQX3), mouse (Q8BUV3), rat (Q03555), pig, goldfish, zebrafish.
Other species not tested yet.
Specificity Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O. validated
Data sheet 147_011c2.pdf

References for Gephyrin - 147 011C2

Cortical somatostatin interneuron subtypes form cell-type-specific circuits.
Wu SJ, Sevier E, Dwivedi D, Saldi GA, Hairston A, Yu S, Abbott L, Choi DH, Sherer M, Qiu Y, Shinde A, et al.
Neuron (2023) 11117: 2675-2692.e9. 147 011C2 IHC; tested species: mouse
Cat. No.: 147 011C2
Amount: 100 µg
Price: $465.00
Cortical somatostatin interneuron subtypes form cell-type-specific circuits.
Wu SJ, Sevier E, Dwivedi D, Saldi GA, Hairston A, Yu S, Abbott L, Choi DH, Sherer M, Qiu Y, Shinde A, et al.
Neuron (2023) 11117: 2675-2692.e9. 147 011C2 IHC; tested species: mouse
ICC
Background

Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.
Other products for this target