|Cat. No. 147 009||
50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: (see remarks)
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 up to 1 : 1000 gallery
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 up to 1 : 2000 (see remarks) gallery
|Subtype||IgY (λ light chain)|
|Immunogen||Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)|
Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reacts with: mouse (Q8BUV3), rat (Q03555), human (Q9NQX3), goldfish, zebrafish, pig.
Other species not tested yet.
|Specificity||Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O. validated|
This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with chicken specific sequences. The antibody can therefore be used with standard anti-chicken secondary reagents.
Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.