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Gephyrin antibody - 147 009 K.O.

Gephyrin is a scaffolding protein associated with inhibitory neurotransmitter receptors
Chicken monoclonal recombinant IgY
Cat. No.: 147 009
Amount: 50 µg
Price: $415.00
Cat. No. 147 009 50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
WB: (see remarks)
IP: (see remarks)
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 2000 (see remarks) gallery  
Clone ChmAb7a
Subtype IgY (λ light chain)
Immunogen Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: mouse (Q8BUV3), rat (Q03555), human (Q9NQX3), goldfish, zebrafish, pig.
Other species not tested yet.
Specificity Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O. validated

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with chicken specific sequences. The antibody can therefore be used with standard anti-chicken secondary reagents.
The antibody has been expressed in mammalian cells.
WB: Clone 3B11 (cat. no. 147 111) is highly recommended.
IP: Clone 3B11 (cat. no. 147 111) is highly recommended.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.
Alternatively, antigen retrieval (10 mM citrate, pH 6.0, overnight at 60°C) can be applied.

Data sheet 147_009.pdf
Cat. No.: 147 009
Amount: 50 µg
Price: $415.00

Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.