Synaptic Systems - Gephyrin

Cat. No.: 147 009

Amount: 50 µg

Price: $415.00

Transient Seizure Clusters and Epileptiform Activity Following Widespread Bilateral Hippocampal Interneuron Ablation.
Dusing MR, LaSarge CL, Drake AW, Westerkamp GC, McCoy C, Hetzer SM, Kraus KL, Pedapati EV, Danzer SC
eNeuro (2024) 114: . 147 009 IHC; tested species: mouse

ICC

IHC

Background

Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.

1 ) Hermann A et al., Biochem. Biophys. Res. Commun. (2001)
2 ) Kirsch J et al., Brain Res. (1993)
3 ) Kirsch J et al., Eur. J. Neurosci. (1993)

Protocols

ICC

IHC

Other products for this target

Cat. No. 147 009	50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: (see remarks) IP: (see remarks) ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 2000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.
Clone	ChmAb7a
Subtype	IgY (λ light chain)
Immunogen	Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop	AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity	Reacts with: mouse (Q8BUV3), rat (Q03555), human (Q9NQX3), goldfish, zebrafish, pig. Other species not tested yet.
Specificity	Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O. validated
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with chicken specific sequences. The antibody can therefore be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells. WB: Clone 3B11 (cat. no. 147 111) is highly recommended. IP: Clone 3B11 (cat. no. 147 111) is highly recommended. IHC: For best results use the protocol of Schneider Gasser et al., 2006. Alternatively, antigen retrieval (10 mM citrate, pH 6.0, overnight at 60°C) can be applied.
Data sheet	147_009.pdf

Gephyrin antibody - 147 009 K.O.

References for Gephyrin - 147 009