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Gephyrin antibody - 147 008

Gephyrin is a scaffolding protein associated with inhibitory neurotransmitter receptors
Monoclonal Recombinant rabbit recombinant IgG
Cat. No.: 147 008
Amount: 50 µg
Price: $425.00
Cat. No. 147 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: yes (see remarks)
IP: yes (see remarks)
ICC: 1 : 500 gallery  
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P/FFPE: not tested yet
Clone RbmAb7a
Subtype IgG1 (κ light chain)
Immunogen Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.
Other species not tested yet.
Specificity Specific for the brain specific 93 kDa splice variant. K.O.
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents.
The antibody has been expressed in mammalian cells.
WB: Clone 3B11 (cat. no. 147 111) is highly recommended.
IP: Clone 3B11 (cat. no. 147 111) is highly recommended.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.
Alternatively antigen retrieval (10 mM citrate, pH 6.0, over night at 60°C) can be applied.

Data sheet 147_008.pdf

References for Gephyrin - 147 008

A proline-rich motif in the large intracellular loop of the glycine receptor α1 subunit interacts with the Pleckstrin homology domain of collybistin.
Breitinger U, Weinländer K, Pechmann Y, Langlhofer G, Enz R, Becker CM, Sticht H, Kneussel M, Villmann C, Breitinger HG
Journal of advanced research (2021) 29: 95-106. 147 008 WB; tested species: mouse
Differential regulation of glycinergic and GABAergic nanocolumns at mixed inhibitory synapses.
Yang X, Le Corronc H, Legendre P, Triller A, Specht CG
EMBO reports (2021) : e52154. 147 008 ICC; tested species: rat
Central Cholinergic Synapse Formation in Optimized Primary Septal-Hippocampal Co-cultures.
Djemil S, Ressel CR, Abdel-Ghani M, Schneeweis AK, Pak DTS
Cellular and molecular neurobiology (2020) : . 147 008 ICC; tested species: rat
Identification of a Core Amino Acid Motif within the α Subunit of GABAARs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures.
Nathanson AJ, Zhang Y, Smalley JL, Ollerhead TA, Rodriguez Santos MA, Andrews PM, Wobst HJ, Moore YE, Brandon NJ, Hines RM, Davies PA, et al.
Cell reports (2019) 283: 670-681.e8. 147 008 ICC; tested species: mouse
Differential Properties of the Synaptogenic Activities of the Neurexin Ligands Neuroligin1 and LRRTM2.
Dagar S, Gottmann K
Frontiers in molecular neuroscience (2019) 12: 269. 147 008 ICC; tested species: mouse
Modeling a Neurexin-3α Human Mutation in Mouse Neurons Identifies a Novel Role in the Regulation of Transsynaptic Signaling and Neurotransmitter Release at Excitatory Synapses.
Restrepo S, Langer NJ, Nelson KA, Aoto J
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) 3946: 9065-9082. 147 008 ICC; tested species: mouse
Elucidating the Molecular Basis for Inhibitory Neurotransmission Regulation by Artemisinins.
Kasaragod VB, Hausrat TJ, Schaefer N, Kuhn M, Christensen NR, Tessmer I, Maric HM, Madsen KL, Sotriffer C, Villmann C, Kneussel M, et al.
Neuron (2019) 1014: 673-689.e11. 147 008 ICC; tested species: mouse
An E3-ligase-based method for ablating inhibitory synapses.
Gross GG, Straub C, Perez-Sanchez J, Dempsey WP, Junge JA, Roberts RW, Trinh le A, Fraser SE, De Koninck Y, De Koninck P, Sabatini BL, et al.
Nature methods (2016) 138: 673-8. 147 008 ICC; tested species: mouse
Activation of Apoptosis in a βB1-CTGF Transgenic Mouse Model.
Weiss M, Reinehr S, Mueller-Buehl AM, Doerner JD, Fuchshofer R, Stute G, Dick HB, Joachim SC
International journal of molecular sciences (2021) 224: . 147 008 IHC; tested species: mouse
Astrocytes phagocytose adult hippocampal synapses for circuit homeostasis.
Lee JH, Kim JY, Noh S, Lee H, Lee SY, Mun JY, Park H, Chung WS
Nature (2021) 5907847: 612-617. 147 008 IHC; tested species: mouse
Microglial mTOR is neuronal protective and anti-epileptogenic in the pilocarpine model of temporal lobe epilepsy.
Zhao XF, Liao Y, Alam MM, Mathur R, Feustel P, Mazurkiewicz JE, Adamo MA, Zhu XC, Huang Y
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 147 008 IHC; tested species: mouse
Transfer of the Experimental Autoimmune Glaucoma Model from Rats to Mice-New Options to Study Glaucoma Disease.
Reinehr S, Reinhard J, Wiemann S, Hesse K, Voss C, Gandej M, Dick HB, Faissner A, Joachim SC
International journal of molecular sciences (2019) 2010: . 147 008 IHC; tested species: mouse
NPAS4 recruits CCK basket cell synapses and enhances cannabinoid-sensitive inhibition in the mouse hippocampus.
Hartzell AL, Martyniuk KM, Brigidi GS, Heinz DA, Djaja NA, Payne A, Bloodgood BL
eLife (2018) 7: . 147 008 IHC; tested species: mouse
Deletion of the Fractalkine Receptor, CX3CR1, Improves Endogenous Repair, Axon Sprouting, and Synaptogenesis after Spinal Cord Injury in Mice.
Freria CM, Hall JC, Wei P, Guan Z, McTigue DM, Popovich PG
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3713: 3568-3587. 147 008 IHC; tested species: mouse
Erythropoietin Stimulates GABAergic Maturation in the Mouse Hippocampus.
Khalid K, Frei J, Aboouf MA, Koester-Hegmann C, Gassmann M, Fritschy JM, Schneider Gasser EM
eNeuro () 81: . 147 008 IHC; tested species: mouse
Cat. No.: 147 008
Quantity: 50 µg
Price: $425.00
A proline-rich motif in the large intracellular loop of the glycine receptor α1 subunit interacts with the Pleckstrin homology domain of collybistin.
Breitinger U, Weinländer K, Pechmann Y, Langlhofer G, Enz R, Becker CM, Sticht H, Kneussel M, Villmann C, Breitinger HG
Journal of advanced research (2021) 29: 95-106. 147 008 WB; tested species: mouse
Differential regulation of glycinergic and GABAergic nanocolumns at mixed inhibitory synapses.
Yang X, Le Corronc H, Legendre P, Triller A, Specht CG
EMBO reports (2021) : e52154. 147 008 ICC; tested species: rat
Central Cholinergic Synapse Formation in Optimized Primary Septal-Hippocampal Co-cultures.
Djemil S, Ressel CR, Abdel-Ghani M, Schneeweis AK, Pak DTS
Cellular and molecular neurobiology (2020) : . 147 008 ICC; tested species: rat
Identification of a Core Amino Acid Motif within the α Subunit of GABAARs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures.
Nathanson AJ, Zhang Y, Smalley JL, Ollerhead TA, Rodriguez Santos MA, Andrews PM, Wobst HJ, Moore YE, Brandon NJ, Hines RM, Davies PA, et al.
Cell reports (2019) 283: 670-681.e8. 147 008 ICC; tested species: mouse
Differential Properties of the Synaptogenic Activities of the Neurexin Ligands Neuroligin1 and LRRTM2.
Dagar S, Gottmann K
Frontiers in molecular neuroscience (2019) 12: 269. 147 008 ICC; tested species: mouse
Modeling a Neurexin-3α Human Mutation in Mouse Neurons Identifies a Novel Role in the Regulation of Transsynaptic Signaling and Neurotransmitter Release at Excitatory Synapses.
Restrepo S, Langer NJ, Nelson KA, Aoto J
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) 3946: 9065-9082. 147 008 ICC; tested species: mouse
Elucidating the Molecular Basis for Inhibitory Neurotransmission Regulation by Artemisinins.
Kasaragod VB, Hausrat TJ, Schaefer N, Kuhn M, Christensen NR, Tessmer I, Maric HM, Madsen KL, Sotriffer C, Villmann C, Kneussel M, et al.
Neuron (2019) 1014: 673-689.e11. 147 008 ICC; tested species: mouse
An E3-ligase-based method for ablating inhibitory synapses.
Gross GG, Straub C, Perez-Sanchez J, Dempsey WP, Junge JA, Roberts RW, Trinh le A, Fraser SE, De Koninck Y, De Koninck P, Sabatini BL, et al.
Nature methods (2016) 138: 673-8. 147 008 ICC; tested species: mouse
Activation of Apoptosis in a βB1-CTGF Transgenic Mouse Model.
Weiss M, Reinehr S, Mueller-Buehl AM, Doerner JD, Fuchshofer R, Stute G, Dick HB, Joachim SC
International journal of molecular sciences (2021) 224: . 147 008 IHC; tested species: mouse
Astrocytes phagocytose adult hippocampal synapses for circuit homeostasis.
Lee JH, Kim JY, Noh S, Lee H, Lee SY, Mun JY, Park H, Chung WS
Nature (2021) 5907847: 612-617. 147 008 IHC; tested species: mouse
Microglial mTOR is neuronal protective and anti-epileptogenic in the pilocarpine model of temporal lobe epilepsy.
Zhao XF, Liao Y, Alam MM, Mathur R, Feustel P, Mazurkiewicz JE, Adamo MA, Zhu XC, Huang Y
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 147 008 IHC; tested species: mouse
Transfer of the Experimental Autoimmune Glaucoma Model from Rats to Mice-New Options to Study Glaucoma Disease.
Reinehr S, Reinhard J, Wiemann S, Hesse K, Voss C, Gandej M, Dick HB, Faissner A, Joachim SC
International journal of molecular sciences (2019) 2010: . 147 008 IHC; tested species: mouse
NPAS4 recruits CCK basket cell synapses and enhances cannabinoid-sensitive inhibition in the mouse hippocampus.
Hartzell AL, Martyniuk KM, Brigidi GS, Heinz DA, Djaja NA, Payne A, Bloodgood BL
eLife (2018) 7: . 147 008 IHC; tested species: mouse
Deletion of the Fractalkine Receptor, CX3CR1, Improves Endogenous Repair, Axon Sprouting, and Synaptogenesis after Spinal Cord Injury in Mice.
Freria CM, Hall JC, Wei P, Guan Z, McTigue DM, Popovich PG
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3713: 3568-3587. 147 008 IHC; tested species: mouse
Erythropoietin Stimulates GABAergic Maturation in the Mouse Hippocampus.
Khalid K, Frei J, Aboouf MA, Koester-Hegmann C, Gassmann M, Fritschy JM, Schneider Gasser EM
eNeuro () 81: . 147 008 IHC; tested species: mouse
Background

Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.