Synaptic Systems - Gephyrin

Cat. No.: 147 008

Amount: 50 µg

Price: $435.00

Cat. No. 147 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: yes (see remarks) IP: yes (see remarks) ICC: 1 : 500 gallery IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery IHC-P: not recommended ExM: yes (see remarks) gallery
Clone	RbmAb7a
Subtype	IgG1 (κ light chain)
Immunogen	Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop	AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity	Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish. Other species not tested yet.
Specificity	Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O. validated
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. WB: Clone 3B11 (cat. no. 147 111) is highly recommended. IP: Clone 3B11 (cat. no. 147 111) is highly recommended. IHC: For best results use the protocol of Schneider Gasser et al., 2006. Alternatively antigen retrieval (10 mM citrate, pH 6.0, over night at 60°C) can be applied. ExM: This antibody has been successfully used for the epitope-preserving magnified analysis of the proteome (eMAP) expansion microscopy method (Park et al. 2021. PMID: 34767453).
Data sheet	147_008.pdf