Glycine receptor antibody - 146 308

An inhibitory receptor in the nervous system

Guinea pig monoclonal recombinant IgG

Cat. No.: 146 308

Amount: 50 µg

Price: $415.00

Cat. No. 146 308	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 (see remarks) gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	GpmAb4a
Subtype	IgG2 (κ light chain)
Immunogen	Nativ Protein corresponding to AA 1 to 457 from rat Glycine receptor α1 (UniProt Id: P07727)
Epitop	AA 96 to 105 from rat Glycine receptor α1 (UniProt Id: P07727)
Reactivity	Reacts with: human (P23415, P23416, P48167), rat (P07727, P22771, P20781), mouse (Q64018, Q7TNC8, P48168), pig, zebrafish. Other species not tested yet.
Specificity	Specific for all glycine receptor subunits.
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb4a. The constant regions of the heavy and light chains have been replaced with Guinea pig specific sequences. The antibody can therefore be used with standard anti-Guinea pig secondary reagents. It also carries a Strep-tag at the C-terminus of the heavy chain.The antibody has been expressed in mammalian cells. IHC: Tissue sections require additional antigen retrieval with methanol/acetic acid prior to antibody incubation. For details see Dumoulin A, Triller A & Dieudonné S (2001).
Data sheet	146_308.pdf

Cat. No.: 146 308

Amount: 50 µg

Price: $415.00

IHC

Background

The inhibitory glycine receptor (GlyR) is a member of the ligand-gated ion channel superfamily of neurotransmitter receptors. It is an oligomeric protein composed of homologous subunits (α 1-4 and β) with four transmembrane segments (M1-M4) each.
It shows a widespread expression profile in brain. Several isoforms and splice variants with distinct pharmacology have been discovered so far.

1 ) Bechade C et al., Eur. J. Neurosci. (1996)
2 ) Becker CM et al., J. Neurosci. (1986)
3 ) Altschuler RA et al., Brain Res. (1986)
4 ) Triller A et al., J. Cell Biol. (1985)
5 ) Graham D et al., Biochemistry (1985)

Protocols

IHC

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