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Glycine receptor alpha 1 - 146 118

An inhibitory receptor in the nervous system
Monoclonal Recombinant rabbit purified IgG
Cat. No.: 146 118
Amount: 50 µg
Price: 415.00 €
Cat. No. 146 118 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: not recommended
IP: not tested yet
ICC: 1 : 200 up to 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: not recommended
Clone RbmAb2b
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 457 from rat Glycine receptor α1 (UniProt Id: P07727)
Epitop Epitop: AA 29 to 39 from rat Glycine receptor α1 (UniProt Id: P07727)
Reactivity Reacts with: human (P23415), rat (P07727), mouse (Q64018), pig, ape.
Other species not tested yet.
Specificity Specific for the glycine receptor α1-subunit.
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone mAb2b. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC: To improve signal strength, antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) is highly recommended.

Data sheet 146_118.pdf
Cat. No.: 146 118
Quantity: 50 µg
Price: 415.00 €
Background
The inhibitory glycine receptor (GlyR) is a member of the ligand-gated ion channel superfamily of neurotransmitter receptors. It is an oligomeric protein composed of homologous subunits (α 1-4 and β) with four transmembrane segments (M1-M4) each.
It shows a widespread expression profile in brain. Several isoforms and splice variants with distinct pharmacology have been discovered so far.