Synaptic Systems - Glycine receptor alpha1

Cat. No.: 146 111

Amount: 100 µg

Price: $420.00

Cat. No. 146 111	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 (see remarks) gallery IHC-P: not recommended EM: yes FLOWCYTOMETRY: yes
Clone	mAb2b
Subtype	IgG1 (κ light chain)
Immunogen	Nativ Protein corresponding to AA 1 to 457 from rat Glycine receptor α1 (UniProt Id: P07727)
Epitop	AA 29 to 39 from rat Glycine receptor α1 (UniProt Id: P07727)
Reactivity	Reacts with: human (P23415), rat (P07727), mouse (Q64018), pig, monkey. Other species not tested yet.
Remarks	IHC: To improve signal strength antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) is highly recommended.
Data sheet	146_111.pdf

Glycine Receptor β-Targeting Autoantibodies Contribute to the Pathology of Autoimmune Diseases.
Wiessler AL, Talucci I, Piro I, Seefried S, Hörlin V, Baykan BB, Tüzün E, Schaefer N, Maric HM, Sommer C, Villmann C, et al.
Neurology(R) neuroimmunology & neuroinflammation (2024) 112: e200187. 146 111 WB, ICC; tested species: human,mouse

Glycine receptor autoantibody binding to the extracellular domain is independent from receptor glycosylation.
Rauschenberger V, Piro I, Kasaragod VB, Hörlin V, Eckes AL, Kluck CJ, Schindelin H, Meinck HM, Wickel J, Geis C, Tüzün E, et al.
Frontiers in molecular neuroscience (2023) 16: 1089101. 146 111 WB, UPTAKE; tested species: human

A proline-rich motif in the large intracellular loop of the glycine receptor α1 subunit interacts with the Pleckstrin homology domain of collybistin.
Breitinger U, Weinländer K, Pechmann Y, Langlhofer G, Enz R, Becker CM, Sticht H, Kneussel M, Villmann C, Breitinger HG
Journal of advanced research (2021) 29: 95-106. 146 111 WB, ICC, UPTAKE

Changes in neuronal excitability and synaptic transmission in nucleus accumbens in a transgenic Alzheimer's disease mouse model.
Fernández-Pérez EJ, Gallegos S, Armijo-Weingart L, Araya A, Riffo-Lepe NO, Cayuman F, Aguayo LG
Scientific reports (2020) 101: 19606. 146 111 WB, IHC; tested species: mouse

Glycine receptor is differentially expressed in the rat retina at early stages of streptozotocin-induced diabetes.
Morales-Calixto E, Velázquez-Flores MÁ, Sánchez-Chávez G, Ruiz Esparza-Garrido R, Salceda R
Neuroscience letters (2019) 712: 134506. 146 111 WB; tested species: rat

Cat. No.: 146 111

Amount: 100 µg

Price: $420.00

ICC

IHC

Background

Protocols

Glycine receptor alpha1 antibody - 146 111