Tailor-made Antibodies
and Tools for Life Science

Glycine receptor antibody - 146 011

An inhibitory receptor in the nervous system
Mouse monoclonal purified IgG
Cat. No.: 146 011
Amount: 100 µg
Price: $425.00
Cat. No. 146 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP staining) gallery  
IP: yes
ICC: yes
IHC: 1 : 250 (see remarks) gallery  
IHC-P: 1 : 100 up to 1 : 500 gallery  
ELISA: yes (see remarks)
FLOWCYTOMETRY: yes
Clone mAb4a
Subtype IgG1 (κ light chain)
Immunogen Nativ Protein corresponding to AA 1 to 457 from rat Glycine receptor α1 (UniProt Id: P07727)
Epitop AA 96 to 105 from rat Glycine receptor α1 (UniProt Id: P07727)
Reactivity Reacts with: human (P23415, P23416, P48167), rat (P07727, P22771, P20781), mouse (Q64018, Q7TNC8, P48168), pig, zebrafish.
Other species not tested yet.
Specificity Specific for all glycine receptor subunits.
Remarks

IHC: Tissue sections require additional antigen retrieval with 95% methanol, 5% acetic acid prior to antibody incubation. For details see Dumoulin A, Triller A & Dieudonné S (2001).
ELISA: The ELISA-protocol for membrane proteins is recommended.

Data sheet 146_011.pdf

References for Glycine receptor - 146 011

Impaired Presynaptic Function Contributes Significantly to the Pathology of Glycine Receptor Autoantibodies.
Wiessler AL, Zheng F, Werner C, Habib M, Tuzun E, Alzheimer C, Sommer C, Villmann C
Neurology(R) neuroimmunology & neuroinflammation (2025) 122: e200364. 146 011 WB, ICC; tested species: mouse
Glycine Receptor β-Targeting Autoantibodies Contribute to the Pathology of Autoimmune Diseases.
Wiessler AL, Talucci I, Piro I, Seefried S, Hörlin V, Baykan BB, Tüzün E, Schaefer N, Maric HM, Sommer C, Villmann C, et al.
Neurology(R) neuroimmunology & neuroinflammation (2024) 112: e200187. 146 011 WB, ICC; tested species: human,mouse
Glycine receptor autoantibody binding to the extracellular domain is independent from receptor glycosylation.
Rauschenberger V, Piro I, Kasaragod VB, Hörlin V, Eckes AL, Kluck CJ, Schindelin H, Meinck HM, Wickel J, Geis C, Tüzün E, et al.
Frontiers in molecular neuroscience (2023) 16: 1089101. 146 011 WB, UPTAKE; tested species: human
Altered expression of synaptic proteins and adhesion molecules in the hippocampus and cortex following the onset of diabetes in nonobese diabetic mice.
Yokokawa T, Kido K, Sato K, Hayashi T, Fujita S
Physiological reports (2023) 118: e15673. 146 011 WB; tested species: mouse
Loss, Gain and Altered Function of GlyR α2 Subunit Mutations in Neurodevelopmental Disorders.
Chen X, Wilson KA, Schaefer N, De Hayr L, Windsor M, Scalais E, van Rijckevorsel G, Stouffs K, Villmann C, O'Mara ML, Lynch JW, et al.
Frontiers in molecular neuroscience (2022) 15: 886729. 146 011 WB; tested species: human
A novel glycine receptor variant with startle disease affects syndapin I and glycinergic inhibition.
Langlhofer G, Schaefer N, Maric HM, Keramidas A, Zhang Y, Baumann P, Blum R, Breitinger U, Strømgaard K, Schlosser A, Kessels MM, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 146 011 WB, ICC; tested species: human
Glycine Receptor Autoantibodies Impair Receptor Function and Induce Motor Dysfunction.
Rauschenberger V, von Wardenburg N, Schaefer N, Ogino K, Hirata H, Lillesaar C, Kluck CJ, Meinck HM, Borrmann M, Weishaupt A, Doppler K, et al.
Annals of neurology (2020) 883: 544-561. 146 011 WB, IHC, UPTAKE; tested species: human,zebrafish
Disruption of a Structurally Important Extracellular Element in the Glycine Receptor Leads to Decreased Synaptic Integration and Signaling Resulting in Severe Startle Disease.
Schaefer N, Berger A, van Brederode J, Zheng F, Zhang Y, Leacock S, Littau L, Jablonka S, Malhotra S, Topf M, Winter F, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3733: 7948-7961. 146 011 WB; tested species: mouse
The GlyR Extracellular β8-β9 Loop - A Functional Determinant of Agonist Potency.
Janzen D, Schaefer N, Delto C, Schindelin H, Villmann C
Frontiers in molecular neuroscience (2017) 10: 322. 146 011 WB, ICC; tested species: human
Gephyrin-binding peptides visualize postsynaptic sites and modulate neurotransmission.
Maric HM, Hausrat TJ, Neubert F, Dalby NO, Doose S, Sauer M, Kneussel M, Strømgaard K
Nature chemical biology (2017) 132: 153-160. 146 011 WB; tested species: mouse
Disturbed neuronal ER-Golgi sorting of unassembled glycine receptors suggests altered subcellular processing is a cause of human hyperekplexia.
Schaefer N, Kluck CJ, Price KL, Meiselbach H, Vornberger N, Schwarzinger S, Hartmann S, Langlhofer G, Schulz S, Schlegel N, Brockmann K, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 351: 422-37. 146 011 ICC, WB
Disturbances of Ligand Potency and Enhanced Degradation of the Human Glycine Receptor at Affected Positions G160 and T162 Originally Identified in Patients Suffering from Hyperekplexia.
Atak S, Langlhofer G, Schaefer N, Kessler D, Meiselbach H, Delto C, Schindelin H, Villmann C
Frontiers in molecular neuroscience (2015) 8: 79. 146 011 WB, ICC; tested species: human
Slowly emerging glycinergic transmission enhances inhibition in the sound localization pathway of the avian auditory system.
Fischl MJ, Weimann SR, Kearse MG, Burger RM
Journal of neurophysiology (2014) 1113: 565-72. 146 011 WB, IHC; tested species: chicken
Single expressed glycine receptor domains reconstitute functional ion channels without subunit-specific desensitization behavior.
Meiselbach H, Vogel N, Langlhofer G, Stangl S, Schleyer B, Bahnassawy L, Sticht H, Breitinger HG, Becker CM, Villmann C
The Journal of biological chemistry (2014) 28942: 29135-47. 146 011 WB, ICC; tested species: mouse,rat
Differential subcellular targeting of glutamate receptor subtypes during homeostatic synaptic plasticity.
Soares C, Lee KF, Nassrallah W, Béïque JC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3333: 13547-59. 146 011 WB; tested species: mouse
Ethanol reduces neuronal excitability of lateral orbitofrontal cortex neurons via a glycine receptor dependent mechanism.
Badanich KA, Mulholland PJ, Beckley JT, Trantham-Davidson H, Woodward JJ
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2013) 387: 1176-88. 146 011 WB
Distribution of the glycine receptor β-subunit in the mouse CNS as revealed by a novel monoclonal antibody.
Weltzien F, Puller C, O'Sullivan GA, Paarmann I, Betz H
The Journal of comparative neurology (2012) 52017: 3962-81. 146 011 WB, ICC, IHC
Age-related changes of glycine receptor at the rat hippocampus: from the embryo to the adult.
Aroeira RI, Ribeiro JA, Sebastião AM, Valente CA
Journal of neurochemistry (2011) 1183: 339-53. 146 011 WB, IHC; tested species: rat
Neuronal cotransport of glycine receptor and the scaffold protein gephyrin.
Maas C, Tagnaouti N, Loebrich S, Behrend B, Lappe-Siefke C, Kneussel M
The Journal of cell biology (2006) 1723: 441-51. 146 011 WB, IP, ICC
Activation of glycine and glutamate receptors increases intracellular calcium in cells derived from the endocrine pancreas.
Weaver CD, Partridge JG, Yao TL, Moates JM, Magnuson MA, Verdoorn TA
Molecular pharmacology (1998) 544: 639-46. 146 011 WB
Activation-Dependent Rapid Postsynaptic Clustering of Glycine Receptors in Mature Spinal Cord Neurons.
Nakahata Y, Eto K, Murakoshi H, Watanabe M, Kuriu T, Hirata H, Moorhouse AJ, Ishibashi H, Nabekura J
eNeuro () 41: . 146 011 WB
Cat. No.: 146 011
Amount: 100 µg
Price: $425.00