Synaptic Systems - Glycine receptor

Cat. No.: 146 008

Amount: 50 µg

Price: $415.00

Cat. No. 146 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 IHC: 1 : 500 up to 1 : 5000 (see remarks) gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	RbmAb4a
Subtype	IgG1 (κ light chain)
Immunogen	Nativ Protein corresponding to AA 1 to 457 from rat Glycine receptor α1 (UniProt Id: P07727)
Epitop	AA 96 to 105 from rat Glycine receptor α1 (UniProt Id: P07727)
Reactivity	Reacts with: human (P23415, P23416, P48167), rat (P07727, P22771, P20781), mouse (Q64018, Q7TNC8, P48168), pig, zebrafish. Other species not tested yet.
Specificity	Specific for all glycine receptor subunits.
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb4a. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. It also carries a Strep-tag at the C-terminus of the heavy chain.The antibody has been expressed in mammalian cells. IHC: Tissue sections require additional antigen retrieval with methanol/acetic acid prior to antibody incubation. For details see Dumoulin A, Triller A & Dieudonné S (2001).
Data sheet	146_008.pdf

References for Glycine receptor - 146 008

Role of the Glycine Receptor β Subunit in Synaptic Localization and Pathogenicity in Severe Startle Disease.
Wiessler AL, Hasenmüller AS, Fuhl I, Mille C, Cortes Campo O, Reinhard N, Schenk J, Heinze KG, Schaefer N, Specht CG, Villmann C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2024) 442: . 146 008 WB; tested species: mouse

Contribution of GlyR α3 Subunits to the Sensitivity and Effect of Ethanol in the Nucleus Accumbens.
San Martin LS, Armijo-Weingart L, Araya A, Yévenes GE, Harvey RJ, Aguayo LG
Frontiers in molecular neuroscience (2021) 14: 756607. 146 008 WB; tested species: mouse

Presence of ethanol-sensitive and ethanol-insensitive glycine receptors in the ventral tegmental area and prefrontal cortex in mice.
Araya A, Gallegos S, Viveros R, San Martin L, Muñoz B, Harvey RJ, Zeilhofer HU, Aguayo LG
British journal of pharmacology (2021) 17823: 4691-4707. 146 008 WB; tested species: mouse

Ethanol consumption and sedation are altered in mice lacking the glycine receptor α2 subunit.
San Martin L, Gallegos S, Araya A, Romero N, Morelli G, Comhair J, Harvey RJ, Rigo JM, Brone B, Aguayo LG
British journal of pharmacology (2020) 17717: 3941-3956. 146 008 WB; tested species: mouse

A zebrafish gephyrinb mutant distinguishes synaptic and enzymatic functions of Gephyrin.
Brennan EJ, Monk KR, Li J
Neural development (2024) 191: 14. 146 008 IHC; tested species: zebrafish

Synaptic input and Ca2+ activity in zebrafish oligodendrocyte precursor cells contribute to myelin sheath formation.
Li J, Miramontes TG, Czopka T, Monk KR
Nature neuroscience (2024) 272: 219-231. 146 008 IHC; tested species: zebrafish

Brain volume increase and neuronal plasticity underly predator-induced morphological defense expression in Daphnia longicephala.
Graeve A, Ioannidou I, Reinhard J, Görl DM, Faissner A, Weiss LC
Scientific reports (2021) 111: 12612. 146 008 IHC

Cat. No.: 146 008

Amount: 50 µg

Price: $415.00

A zebrafish gephyrinb mutant distinguishes synaptic and enzymatic functions of Gephyrin.
Brennan EJ, Monk KR, Li J
Neural development (2024) 191: 14. 146 008 IHC; tested species: zebrafish

IHC

IHC-P

Background

The inhibitory glycine receptor (GlyR) is a member of the ligand-gated ion channel superfamily of neurotransmitter receptors. It is an oligomeric protein composed of homologous subunits (α 1-4 and β) with four transmembrane segments (M1-M4) each.
It shows a widespread expression profile in brain. Several isoforms and splice variants with distinct pharmacology have been discovered so far.

1 ) Bechade C et al., Eur. J. Neurosci. (1996)
2 ) Becker CM et al., J. Neurosci. (1986)
3 ) Altschuler RA et al., Brain Res. (1986)
4 ) Triller A et al., J. Cell Biol. (1985)
5 ) Graham D et al., Biochemistry (1985)

Protocols

ICC

IHC

IHC-P

Other products for this target

Glycine receptor antibody - 146 008

References for Glycine receptor - 146 008