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Glycine receptor - 146 008

An inhibitory receptor in the nervous system
Monoclonal Recombinant rabbit purified IgG
Cat. No.: 146 008
Amount: 50 µg
Price: 415.00 €
Cat. No. 146 008 50 µg purified IgG, lyophilized. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: 1 : 500 gallery  
Clone RbmAb4a
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 457 from rat Glycine receptor α1 (UniProt Id: P07727)
Epitop Epitop: AA 96 to 105 from rat Glycine receptor α1 (UniProt Id: P07727)
Reactivity Reacts with: human (P23415, P23416, P48167), rat (P07727, P22771, P20781), mouse (Q64018, Q7TNC8, P48168), pig, zebrafish.
Other species not tested yet.
Specificity Specific for all glycine receptor subunits.
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb4a. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. It also carries a Strep-tag at the C-terminus of the heavy chain.The antibody has been expressed in mammalian cells.
IHC: Tissue sections require additional antigen retrieval with methanol/acetic acid prior to antibody incubation. For details see Dumoulin A, Triller A & Dieudonné S (2001).

Data sheet 146_008.pdf

References for Glycine receptor - 146 008

Ethanol consumption and sedation are altered in mice lacking the glycine receptor α2 subunit.
San Martin L, Gallegos S, Araya A, Romero N, Morelli G, Comhair J, Harvey RJ, Rigo JM, Brone B, Aguayo LG
British journal of pharmacology (2020) 17717: 3941-3956. 146 008 WB; tested species: mouse
Cat. No.: 146 008
Quantity: 50 µg
Price: 415.00 €
Ethanol consumption and sedation are altered in mice lacking the glycine receptor α2 subunit.
San Martin L, Gallegos S, Araya A, Romero N, Morelli G, Comhair J, Harvey RJ, Rigo JM, Brone B, Aguayo LG
British journal of pharmacology (2020) 17717: 3941-3956. 146 008 WB; tested species: mouse
Background
The inhibitory glycine receptor (GlyR) is a member of the ligand-gated ion channel superfamily of neurotransmitter receptors. It is an oligomeric protein composed of homologous subunits (α 1-4 and β) with four transmembrane segments (M1-M4) each.
It shows a widespread expression profile in brain. Several isoforms and splice variants with distinct pharmacology have been discovered so far.