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Synaptojanin1 antibody C-terminus - 145 003

Synaptojanin1 is a phosphoinositide phosphatase
Rabbit polyclonal purified antibody
Cat. No.: 145 003
Amount: 50 µg
Price: $375.00
Cat. No. 145 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 200 up to 1 : 2000 (AP staining) gallery  
IP: not tested yet
ICC: yes
IHC: yes
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to AA 1292 to 1308 from rat Synaptojanin1 (UniProt Id: Q62910-1)
Reactivity Reacts with: rat (Q62910), mouse (Q8CHC4), hamster.
Other species not tested yet.
Specificity Recognizes all isoforms of synaptojanin 1.
Matching control protein/peptide 145-0P
Remarks

Running SDS-PAGE with non-boiled samples gives stronger signals.

Data sheet 145_003.pdf

References for Synaptojanin1 - 145 003

Synaptic AP2 CCV life cycle regulation by the Eps15, ITSN1, Sgip1/AP2, synaptojanin1 interactome.
Mishra R, Sengül GF, Candiello E, Schu P
Scientific reports (2021) 111: 8007. 145 003 WB; tested species: mouse
Proteomic Analysis After Status Epilepticus Identifies UCHL1 as Protective Against Hippocampal Injury.
Reynolds JP, Jimenez-Mateos EM, Cao L, Bian F, Alves M, Miller-Delaney SF, Zhou A, Henshall DC
Neurochemical research (2017) 427: 2033-2054. 145 003 WB
Synaptic function is modulated by LRRK2 and glutamate release is increased in cortical neurons of G2019S LRRK2 knock-in mice.
Beccano-Kelly DA, Kuhlmann N, Tatarnikov I, Volta M, Munsie LN, Chou P, Cao LP, Han H, Tapia L, Farrer MJ, Milnerwood AJ, et al.
Frontiers in cellular neuroscience (2014) 8: 301. 145 003 WB; tested species: mouse
Efficient synaptic vesicle recycling after intense exocytosis concomitant with the accumulation of non-releasable endosomes at early developmental stages.
Bartolomé-Martín D, Ramírez-Franco J, Castro E, Sánchez-Prieto J, Torres M
Journal of cell science (2012) 125Pt 2: 422-34. 145 003 WB; tested species: rat
Molecular anatomy of the hair cell's ribbon synapse.
Uthaiah RC, Hudspeth AJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3037: 12387-99. 145 003 WB
SNX18 shares a redundant role with SNX9 and modulates endocytic trafficking at the plasma membrane.
Park J, Kim Y, Lee S, Park JJ, Park ZY, Sun W, Kim H, Chang S
Journal of cell science (2010) 123Pt 10: 1742-50. 145 003 WB
Sorting nexin 9 interacts with dynamin 1 and N-WASP and coordinates synaptic vesicle endocytosis.
Shin N, Lee S, Ahn N, Kim SA, Ahn SG, YongPark Z, Chang S
The Journal of biological chemistry (2007) 28239: 28939-50. 145 003 WB
Human autoantibodies to amphiphysin induce defective presynaptic vesicle dynamics and composition.
Werner C, Pauli M, Doose S, Weishaupt A, Haselmann H, Grünewald B, Sauer M, Heckmann M, Toyka KV, Asan E, Sommer C, et al.
Brain : a journal of neurology (2016) 139Pt 2: 365-79. 145 003 ICC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 145 003 ICC, IHC; tested species: mouse,rat
Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 145 003 IHC, EM; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 145 003 ICC, IHC; tested species: mouse,rat
Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 145 003 IHC, EM; tested species: rat
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 145 003
Cat. No.: 145 003
Amount: 50 µg
Price: $375.00
Synaptic AP2 CCV life cycle regulation by the Eps15, ITSN1, Sgip1/AP2, synaptojanin1 interactome.
Mishra R, Sengül GF, Candiello E, Schu P
Scientific reports (2021) 111: 8007. 145 003 WB; tested species: mouse
Proteomic Analysis After Status Epilepticus Identifies UCHL1 as Protective Against Hippocampal Injury.
Reynolds JP, Jimenez-Mateos EM, Cao L, Bian F, Alves M, Miller-Delaney SF, Zhou A, Henshall DC
Neurochemical research (2017) 427: 2033-2054. 145 003 WB
Synaptic function is modulated by LRRK2 and glutamate release is increased in cortical neurons of G2019S LRRK2 knock-in mice.
Beccano-Kelly DA, Kuhlmann N, Tatarnikov I, Volta M, Munsie LN, Chou P, Cao LP, Han H, Tapia L, Farrer MJ, Milnerwood AJ, et al.
Frontiers in cellular neuroscience (2014) 8: 301. 145 003 WB; tested species: mouse
Efficient synaptic vesicle recycling after intense exocytosis concomitant with the accumulation of non-releasable endosomes at early developmental stages.
Bartolomé-Martín D, Ramírez-Franco J, Castro E, Sánchez-Prieto J, Torres M
Journal of cell science (2012) 125Pt 2: 422-34. 145 003 WB; tested species: rat
Molecular anatomy of the hair cell's ribbon synapse.
Uthaiah RC, Hudspeth AJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3037: 12387-99. 145 003 WB
SNX18 shares a redundant role with SNX9 and modulates endocytic trafficking at the plasma membrane.
Park J, Kim Y, Lee S, Park JJ, Park ZY, Sun W, Kim H, Chang S
Journal of cell science (2010) 123Pt 10: 1742-50. 145 003 WB
Sorting nexin 9 interacts with dynamin 1 and N-WASP and coordinates synaptic vesicle endocytosis.
Shin N, Lee S, Ahn N, Kim SA, Ahn SG, YongPark Z, Chang S
The Journal of biological chemistry (2007) 28239: 28939-50. 145 003 WB
Human autoantibodies to amphiphysin induce defective presynaptic vesicle dynamics and composition.
Werner C, Pauli M, Doose S, Weishaupt A, Haselmann H, Grünewald B, Sauer M, Heckmann M, Toyka KV, Asan E, Sommer C, et al.
Brain : a journal of neurology (2016) 139Pt 2: 365-79. 145 003 ICC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 145 003 ICC, IHC; tested species: mouse,rat
Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 145 003 IHC, EM; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 145 003 ICC, IHC; tested species: mouse,rat
Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 145 003 IHC, EM; tested species: rat
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 145 003
Background

Synaptojanin 1 is a phosphoinositide phosphatase which exists in two tissue specific isoforms (170 and 145 kDa). The 145 kDa isoform is predominantly expressed in the nervous system. It has a three domain structure with an N-terminal part homologous to the yeast Sac 1p protein, a central inositol 5-phosphatase domain and a C-terminal SH3 binding domain. Synaptojanin is involved in clathrin mediated synaptic vesicle recycling and binds to endophilin and amphiphysin.
Six isoforms are described so far and two splice variants of the 145 kDa isoform. The predominant one contains a 16 AA insert at position 1140-1155.