Synaptic Systems - Synaptojanin1

Cat. No.: 145 003

Amount: 50 µg

Price: $375.00

Cat. No. 145 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 200 up to 1 : 2000 (AP staining) (see remarks) gallery IP: not tested yet ICC: yes IHC: yes IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1292 to 1308 from rat Synaptojanin1 (UniProt Id: Q62910-1)
Reactivity	Reacts with: rat (Q62910), mouse (Q8CHC4), hamster. Other species not tested yet.
Specificity	Recognizes all isoforms of synaptojanin 1.
Matching control protein/peptide	145-0P
Remarks	WB: Running SDS-PAGE with non-boiled samples gives stronger signals.
Data sheet	145_003.pdf

References for Synaptojanin1 - 145 003

Synaptic AP2 CCV life cycle regulation by the Eps15, ITSN1, Sgip1/AP2, synaptojanin1 interactome.
Mishra R, Sengül GF, Candiello E, Schu P
Scientific reports (2021) 111: 8007. 145 003 WB; tested species: mouse

Proteomic Analysis After Status Epilepticus Identifies UCHL1 as Protective Against Hippocampal Injury.
Reynolds JP, Jimenez-Mateos EM, Cao L, Bian F, Alves M, Miller-Delaney SF, Zhou A, Henshall DC
Neurochemical research (2017) 427: 2033-2054. 145 003 WB

Synaptic function is modulated by LRRK2 and glutamate release is increased in cortical neurons of G2019S LRRK2 knock-in mice.
Beccano-Kelly DA, Kuhlmann N, Tatarnikov I, Volta M, Munsie LN, Chou P, Cao LP, Han H, Tapia L, Farrer MJ, Milnerwood AJ, et al.
Frontiers in cellular neuroscience (2014) 8: 301. 145 003 WB; tested species: mouse

Efficient synaptic vesicle recycling after intense exocytosis concomitant with the accumulation of non-releasable endosomes at early developmental stages.
Bartolomé-Martín D, Ramírez-Franco J, Castro E, Sánchez-Prieto J, Torres M
Journal of cell science (2012) 125Pt 2: 422-34. 145 003 WB; tested species: rat

Molecular anatomy of the hair cell's ribbon synapse.
Uthaiah RC, Hudspeth AJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3037: 12387-99. 145 003 WB

SNX18 shares a redundant role with SNX9 and modulates endocytic trafficking at the plasma membrane.
Park J, Kim Y, Lee S, Park JJ, Park ZY, Sun W, Kim H, Chang S
Journal of cell science (2010) 123Pt 10: 1742-50. 145 003 WB

Sorting nexin 9 interacts with dynamin 1 and N-WASP and coordinates synaptic vesicle endocytosis.
Shin N, Lee S, Ahn N, Kim SA, Ahn SG, YongPark Z, Chang S
The Journal of biological chemistry (2007) 28239: 28939-50. 145 003 WB

Human autoantibodies to amphiphysin induce defective presynaptic vesicle dynamics and composition.
Werner C, Pauli M, Doose S, Weishaupt A, Haselmann H, Grünewald B, Sauer M, Heckmann M, Toyka KV, Asan E, Sommer C, et al.
Brain : a journal of neurology (2016) 139Pt 2: 365-79. 145 003 ICC; tested species: rat

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 145 003 ICC, IHC; tested species: mouse,rat

Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 145 003 IHC, EM; tested species: rat

Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 145 003

Cat. No.: 145 003

Amount: 50 µg

Price: $375.00

Molecular anatomy of the hair cell's ribbon synapse.
Uthaiah RC, Hudspeth AJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3037: 12387-99. 145 003 WB

Background

Synaptojanin 1 is a phosphoinositide phosphatase which exists in two tissue specific isoforms (170 and 145 kDa). The 145 kDa isoform is predominantly expressed in the nervous system. It has a three domain structure with an N-terminal part homologous to the yeast Sac 1p protein, a central inositol 5-phosphatase domain and a C-terminal SH3 binding domain. Synaptojanin is involved in clathrin mediated synaptic vesicle recycling and binds to endophilin and amphiphysin.
Six isoforms are described so far and two splice variants of the 145 kDa isoform. The predominant one contains a 16 AA insert at position 1140-1155.

1 ) Lee SY et al., Proc. Natl. Acad. Sci. U.S.A. (2004)
2 ) Schuske KR et al., Neuron (2003)
3 ) Verstreken P et al., Neuron (2003)
4 ) Song W et al., Neuron (2003)
5 ) Luthi A et al., J. Neurosci. (2001)
6 ) Harris TW et al., J. Cell Biol. (2000)
7 ) Haffner C et al., Curr. Biol. (2000)
8 ) Woscholski R et al., J. Biol. Chem. (1997)
9 ) de Heuvel E et al., J. Biol. Chem. (1997)
10 ) Ramjaun AR et al., J. Biol. Chem. (1996)

Protocols

ICC

IHC

OTHER

Preabsorbtion

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Synaptojanin1 antibody C-terminus - 145 003

References for Synaptojanin1 - 145 003