Cat. No.: 145 003
Amount: 50 µg
Price:
$375.00
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| Cat. No. 145 003 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
IP: not tested yet ICC: yes IHC: yes IHC-P: not tested yet |
| Immunogen | Synthetic peptide corresponding to AA 1292 to 1308 from rat Synaptojanin1 (UniProt Id: Q62910-1) |
| Reactivity |
Reacts with: rat (Q62910), mouse (Q8CHC4), hamster. Other species not tested yet. |
| Specificity | Recognizes all isoforms of synaptojanin 1. |
| Matching control protein/peptide | 145-0P |
| Remarks |
WB: Running SDS-PAGE with non-boiled samples gives stronger signals. |
| Data sheet | 145_003.pdf |
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Synaptojanin 1 is a phosphoinositide phosphatase which exists in two tissue specific isoforms (170 and 145 kDa). The 145 kDa isoform is predominantly expressed in the nervous system. It has a three domain structure with an N-terminal part homologous to the yeast Sac 1p protein, a central inositol 5-phosphatase domain and a C-terminal SH3 binding domain. Synaptojanin is involved in clathrin mediated synaptic vesicle recycling and binds to endophilin and amphiphysin.
Six isoforms are described so far and two splice variants of the 145 kDa isoform. The predominant one contains a 16 AA insert at position 1140-1155.