Tailor-made Antibodies
and Tools for Life Science

Piccolo antibody - 142 104 K.O.

Piccolo is a large multi-domain protein of the presynaptic active zone
Guinea pig polyclonal antiserum
Cat. No.: 142 104
Amount: 100 µl
Price: $370.00
Cat. No. 142 104 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 200 gallery  
IHC-P: 1 : 500 gallery  
Immunogen Recombinant protein corresponding to a central region of rat piccolo (UniProt Id: Q9JKS6)
Reactivity Reacts with: rat (Q9JKS6), mouse (Q9QYX7).
Other species not tested yet.
Specificity K.O. validated PubMed: 32122952
Remarks

WB: Due to its large size, piccolo requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.

This antibody detects an additional band of ~65 kDa.

Data sheet 142_104.pdf

References for Piccolo - 142 104

Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting.
Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, Heukamp A, Rannio S, Martens H, Barth J, Burk K, et al.
Science (New York, N.Y.) (2018) : . 142 104 WB; tested species: rat
Cat. No.: 142 104
Amount: 100 µl
Price: $370.00