Piccolo antibody - 142 008

Piccolo is a large multi-domain protein of the presynaptic active zone

Rabbit monoclonal recombinant IgG

Cat. No.: 142 008

Amount: 100 µg

Price: $415.00

Cat. No. 142 008	100 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended (see remarks) IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 100 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb294E1
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the central region of rat Piccolo (UniProt Id: Q9JKS6)
Reactivity	Reacts with: mouse (Q9QYX7-1), rat (Q9JKS6). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody SY-294E1. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. WB: This antibody is less sensitive than the polyclonal product 142 113. We recommend 142 113 for westernblotting. Due to its large size, Piccolo requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from Anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from Invitrogen.
Data sheet	142_008.pdf

Cat. No.: 142 008

Amount: 100 µg

Price: $415.00

ICC

IHC

IHC-P

Background

Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn²⁺ finger, several piccolo-bassoon homology domains (PBH-domains) and C-terminal PDZ and C2 domains. In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Piccolo is supposed to be a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Recently piccolo has been shown to interfere with clathrin mediated endocytosis by binding to the F-actin and dynamin binding protein Abp1.

1 ) Shapira M et al., Neuron (2003)
2 ) Fenster SD et al., J. Biol. Chem. (2003)
3 ) Fenster SD et al., Int. J. Dev. Neurosci. ()
4 ) Dick O et al., J. Comp. Neurol. (2001)
5 ) Gerber SH et al., EMBO J. (2001)
6 ) Fenster SD et al., Neuron (2000)
7 ) Cases-Langhoff C et al., Eur. J. Cell Biol. (1996)

Protocols

ICC

IHC

IHC-P

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