Bassoon antibody - 141 118 K.O.

Bassoon is a large multi-domain protein of the presynaptic active zone

Rabbit monoclonal recombinant IgG

Cat. No.: 141 118

Amount: 50 µg

Price: $415.00

Cat. No. 141 118	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 200 up to 1 : 1000 gallery IHC-G: (see remarks) gallery ExM: yes (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence. Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.
Clone	Rb179H11A2
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the central region of rat Bassoon. (UniProt Id: O88778)
Reactivity	Reacts with: mouse (O88737), rat (O88778). Other species not tested yet.
Specificity	specific for Bassoon K.O. validated
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 179H11A2. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen. IHC-G: Fixation with 9% glyoxal, 8% acetic acid, in ddH2O, pH 4.2-4.4, according to Konno et al. 2023 is recommended. ExM: This antibody has been successfully used for the epitope-preserving magnified analysis of the proteome (eMAP) expansion microscopy method (Park et al. 2021. PMID: 34767453).
Data sheet	141_118.pdf

Cat. No.: 141 118

Amount: 50 µg

Price: $415.00

ICC

IHC

IHC-P

IHC-G

ExM

Background

Bassoon is a large protein which consists of an N-terminal Zn²⁺ finger and several piccolo-bassoon homology domains (PBH-domains). It is generally found together with piccolo, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Bassoon was suggested to be a scaffolding element of the presynapse but deletion experiments in mice have shown that bassoon is also involved in synaptic vesicle cycling. Probably bassoon interacts with other protein factors via its Zn²⁺ domain but the potential partners have not been determined yet.

1 ) Dresbach T et al., Mol. Cell. Neurosci. (2003)
2 ) Shapira M et al., Neuron (2003)
3 ) Altrock WD et al., Neuron (2003)
4 ) Dick O et al., Neuron (2003)
5 ) Dick O et al., J. Comp. Neurol. (2001)
6 ) Sanmartí-Vila L et al., Biochem. Biophys. Res. Commun. (2000)
7 ) tom Dieck S et al., J. Cell Biol. (1998)

Protocols

ICC

IHC

IHC-P

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