|Cat. No. 141 118||50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) (see remarks) gallery
IP: not tested yet
ICC: 1 : 500 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 200 up to 1 : 1000 gallery
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 756 to 1001 from rat Bassoon (UniProt Id: O88778)|
Reacts with: mouse (O88737), rat (O88778).
Other species not tested yet.
|Specificity||specific for Bassoon|
This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 179H11A2. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
Bassoon is a large protein which consists of an N-terminal Zn2+ finger and several piccolo-bassoon homology domains (PBH-domains). It is generally found together with piccolo, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Bassoon was suggested to be a scaffolding element of the presynapse but deletion experiments in mice have shown that bassoon is also involved in synaptic vesicle cycling. Probably bassoon interacts with other protein factors via its Zn2+ domain but the potential partners have not been determined yet.