Synaptic Systems

Cat. No.: 141 111

Amount: 100 µg

Price: $415.00

Cat. No. 141 111	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 AP-staining (see remarks) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 up to 1 : 1000 gallery DNA-PAINT: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. In antibody-based DNA-PAINT (Point Accumulation in Nanoscale Topography), a short oligomeric docking strand is coupled to a specific antibody. The transient association of the fluorophore to the antibody is mediated by the pairing of a short fluorescently labeled complementary imager DNA strand. DNA-PAINT allows super-resolution imaging and the imaging of a huge number of antibodies on the same biological sample in a single multiplex experiment. For additional experimental details refer to the Remarks section.
Clone	179H11A2
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the central region of rat Bassoon. (UniProt Id: O88778)
Reactivity	Reacts with: mouse (O88737), rat (O88778). Other species not tested yet.
Specificity	specific for Bassoon K.O. validated PubMed: 33811381
Remarks	WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen. DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).
Data sheet	141_111.pdf

References for Bassoon - 141 111

Loss of microglial MCT4 leads to defective synaptic pruning and anxiety-like behavior in mice.
Monsorno K, Ginggen K, Ivanov A, Buckinx A, Lalive AL, Tchenio A, Benson S, Vendrell M, D'Alessandro A, Beule D, Pellerin L, et al.
Nature communications (2023) 141: 5749. 141 111 WB; tested species: mouse

Genetic disruption of bassoon in two mutant mouse lines causes divergent retinal phenotypes.
Ryl M, Urbasik A, Gierke K, Babai N, Joachimsthaler A, Feigenspan A, Frischknecht R, Stallwitz N, Fejtová A, Kremers J, von Wittgenstein J, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 355: e21520. 141 111 WB; KO verified; tested species: mouse

The first synapse in vision in the aging mouse retina.
Gierke K, Lux UT, Regus-Leidig H, Brandstätter JH
Frontiers in cellular neuroscience (2023) 17: 1291054. 141 111 IHC; tested species: mouse

Kv3.3 subunits control presynaptic action potential waveform and neurotransmitter release at a central excitatory synapse.
Richardson A, Ciampani V, Stancu M, Bondarenko K, Newton S, Steinert JR, Pilati N, Graham BP, Kopp-Scheinpflug C, Forsythe ID
eLife (2022) 11: . 141 111 IHC; tested species: mouse

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 141 111 DNA-PAINT; tested species: rat

Cat. No.: 141 111

Amount: 100 µg

Price: $415.00

The first synapse in vision in the aging mouse retina.
Gierke K, Lux UT, Regus-Leidig H, Brandstätter JH
Frontiers in cellular neuroscience (2023) 17: 1291054. 141 111 IHC; tested species: mouse

ICC

IHC

IHC-P

Background

Bassoon is a large protein which consists of an N-terminal Zn²⁺ finger and several piccolo-bassoon homology domains (PBH-domains). It is generally found together with piccolo, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Bassoon was suggested to be a scaffolding element of the presynapse but deletion experiments in mice have shown that bassoon is also involved in synaptic vesicle cycling. Probably bassoon interacts with other protein factors via its Zn²⁺ domain but the potential partners have not been determined yet.

1 ) Dresbach T et al., Mol. Cell. Neurosci. (2003)
2 ) Shapira M et al., Neuron (2003)
3 ) Altrock WD et al., Neuron (2003)
4 ) Dick O et al., Neuron (2003)
5 ) Dick O et al., J. Comp. Neurol. (2001)
6 ) Sanmartí-Vila L et al., Biochem. Biophys. Res. Commun. (2000)
7 ) tom Dieck S et al., J. Cell Biol. (1998)

Protocols

ICC

IHC

IHC-P

Other products for this target

Bassoon antibody - 141 111 K.O.

References for Bassoon - 141 111