Tailor-made Antibodies
and Tools for Life Science

Bassoon antibody - 141 111 K.O.

Bassoon is a large multi-domain protein of the presynaptic active zone
Mouse monoclonal purified IgG
Cat. No.: 141 111
Amount: 100 µg
Price: $420.00
Cat. No. 141 111 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 AP-staining (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 up to 1 : 1000 gallery  
DNA-PAINT: yes (see remarks)
Clone 179H11
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to residues near the central region of rat Bassoon. (UniProt Id: O88778)
Reactivity Reacts with: mouse (O88737), rat (O88778).
Other species not tested yet.
Specificity specific for Bassoon K.O. validated PubMed: 33811381
Remarks

WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).

Data sheet 141_111.pdf

References for Bassoon - 141 111

Loss of microglial MCT4 leads to defective synaptic pruning and anxiety-like behavior in mice.
Monsorno K, Ginggen K, Ivanov A, Buckinx A, Lalive AL, Tchenio A, Benson S, Vendrell M, D'Alessandro A, Beule D, Pellerin L, et al.
Nature communications (2023) 141: 5749. 141 111 WB; tested species: mouse
Genetic disruption of bassoon in two mutant mouse lines causes divergent retinal phenotypes.
Ryl M, Urbasik A, Gierke K, Babai N, Joachimsthaler A, Feigenspan A, Frischknecht R, Stallwitz N, Fejtová A, Kremers J, von Wittgenstein J, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 355: e21520. 141 111 WB; KO verified; tested species: mouse
Cat. No.: 141 111
Amount: 100 µg
Price: $420.00