|Cat. No. 141 111||100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 AP-staining (see remarks) gallery
IP: not tested yet
ICC: 1 : 500 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 500 up to 1 : 1000 gallery
|Subtype||IgG2a (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 756 to 1001 from rat Bassoon (UniProt Id: O88778)|
Epitop: AA 756 to 1001 from rat Bassoon (UniProt Id: O88778)
Reacts with: mouse (O88737), rat (O88778).
Other species not tested yet.
|Specificity||specific for Bassoon K.O. PubMed: 33811381|
WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
Bassoon is a large protein which consists of an N-terminal Zn2+ finger and several piccolo-bassoon homology domains (PBH-domains). It is generally found together with piccolo, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Bassoon was suggested to be a scaffolding element of the presynapse but deletion experiments in mice have shown that bassoon is also involved in synaptic vesicle cycling. Probably bassoon interacts with other protein factors via its Zn2+ domain but the potential partners have not been determined yet.