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Bassoon antibody - 141 111

Bassoon is a large multi-domain protein of the presynaptic active zone
Monoclonal mouse purified IgG
Cat. No.: 141 111
Amount: 100 µg
Price: $415.00
Cat. No. 141 111 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 AP-staining (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 500 up to 1 : 1000 gallery  
Clone 179H11A2
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 756 to 1001 from rat Bassoon (UniProt Id: O88778)
Epitop Epitop: AA 756 to 1001 from rat Bassoon (UniProt Id: O88778)
Reactivity Reacts with: mouse (O88737), rat (O88778).
Other species not tested yet.
Specificity specific for Bassoon K.O. PubMed: 33811381
Remarks

WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.

Data sheet 141_111.pdf

References for Bassoon - 141 111

Genetic disruption of bassoon in two mutant mouse lines causes divergent retinal phenotypes.
Ryl M, Urbasik A, Gierke K, Babai N, Joachimsthaler A, Feigenspan A, Frischknecht R, Stallwitz N, Fejtová A, Kremers J, von Wittgenstein J, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 355: e21520. 141 111 WB; KO verified; tested species: mouse
Cat. No.: 141 111
Quantity: 100 µg
Price: $415.00
Genetic disruption of bassoon in two mutant mouse lines causes divergent retinal phenotypes.
Ryl M, Urbasik A, Gierke K, Babai N, Joachimsthaler A, Feigenspan A, Frischknecht R, Stallwitz N, Fejtová A, Kremers J, von Wittgenstein J, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 355: e21520. 141 111 WB; KO verified; tested species: mouse
Background

Bassoon is a large protein which consists of an N-terminal Zn2+ finger and several piccolo-bassoon homology domains (PBH-domains). It is generally found together with piccolo, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Bassoon was suggested to be a scaffolding element of the presynapse but deletion experiments in mice have shown that bassoon is also involved in synaptic vesicle cycling. Probably bassoon interacts with other protein factors via its Zn2+ domain but the potential partners have not been determined yet.