Synaptic Systems

Cat. No.: 141 021

Amount: 200 µl

Price: $360.00

Cat. No. 141 021	200 µl hybridoma supernatant, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 100 up to 1 : 500 (see remarks) gallery IHC: not recommended IHC-P: 1 : 500 gallery ELISA: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Clone	219E1
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat Bassoon. (UniProt Id: O88778)
Reactivity	Reacts with: rat (O88778), mouse (O88737). Other species not tested yet.
Specificity	Specific for Bassoon
Matching control protein/peptide	141-0P
Remarks	WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen. ICC: Mild fixation conditions (2 % PFA for 20 min) are recommended. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 141 003 as detector antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet	141_021.pdf

References for Bassoon - 141 021

LAR receptor phospho-tyrosine phosphatases regulate NMDA-receptor responses.
Sclip A, Südhof TC
eLife (2020) 9: . 141 021 WB; tested species: mouse

Microtubule-associated protein 1B (MAP1B)-deficient neurons show structural presynaptic deficiencies in vitro and altered presynaptic physiology.
Bodaleo FJ, Montenegro-Venegas C, Henríquez DR, Court FA, Gonzalez-Billault C
Scientific reports (2016) 6: 30069. 141 021 WB; tested species: mouse

Regulation of presynaptic anchoring of the scaffold protein Bassoon by phosphorylation-dependent interaction with 14-3-3 adaptor proteins.
Schröder MS, Stellmacher A, Romorini S, Marini C, Montenegro-Venegas C, Altrock WD, Gundelfinger ED, Fejtova A
PloS one (2013) 83: e58814. 141 021 WB

Differential Scaling of Synaptic Molecules within Functional Zones of an Excitatory Synapse during Homeostatic Plasticity.
Venkatesan S, Subramaniam S, Rajeev P, Chopra Y, Jose M, Nair D
eNeuro (2020) 72: . 141 021 ICC; tested species: rat

Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 141 021 ICC; tested species: mouse

The Exocyst Component Exo70 Modulates Dendrite Arbor Formation, Synapse Density, and Spine Maturation in Primary Hippocampal Neurons.
Lira M, Arancibia D, Orrego PR, Montenegro-Venegas C, Cruz Y, García J, Leal-Ortiz S, Godoy JA, Gundelfinger ED, Inestrosa NC, Garner CC, et al.
Molecular neurobiology (2018) : . 141 021 ICC; tested species: rat

Nanoscale Structural Plasticity of the Active Zone Matrix Modulates Presynaptic Function.
Glebov OO, Jackson RE, Winterflood CM, Owen DM, Barker EA, Doherty P, Ewers H, Burrone J
Cell reports (2017) 1811: 2715-2728. 141 021 ICC

Effects of trace metal profiles characteristic for autism on synapses in cultured neurons.
Hagmeyer S, Mangus K, Boeckers TM, Grabrucker AM
Neural plasticity (2015) 2015: 985083. 141 021 ICC

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 141 021 ICC; tested species: rat

Synapsin selectively controls the mobility of resting pool vesicles at hippocampal terminals.
Orenbuch A, Shalev L, Marra V, Sinai I, Lavy Y, Kahn J, Burden JJ, Staras K, Gitler D
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3212: 3969-80. 141 021 ICC

Interleukin-4 receptor signaling modulates neuronal network activity.
Hanuscheck N, Thalman C, Domingues M, Schmaul S, Muthuraman M, Hetsch F, Ecker M, Endle H, Oshaghi M, Martino G, Kuhlmann T, et al.
The Journal of experimental medicine (2022) 2196: . 141 021 IHC; tested species: mouse

TRPM1 is required for the depolarizing light response in retinal ON-bipolar cells.
Morgans CW, Zhang J, Jeffrey BG, Nelson SM, Burke NS, Duvoisin RM, Brown RL
Proceedings of the National Academy of Sciences of the United States of America (2009) 10645: 19174-8. 141 021 IHC; tested species: mouse

Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 141 021 ELISA

Cat. No.: 141 021

Amount: 200 µl

Price: $360.00

LAR receptor phospho-tyrosine phosphatases regulate NMDA-receptor responses.
Sclip A, Südhof TC
eLife (2020) 9: . 141 021 WB; tested species: mouse

Effects of trace metal profiles characteristic for autism on synapses in cultured neurons.
Hagmeyer S, Mangus K, Boeckers TM, Grabrucker AM
Neural plasticity (2015) 2015: 985083. 141 021 ICC

ICC

IHC-P

Background

Bassoon is a large protein which consists of an N-terminal Zn²⁺ finger and several piccolo-bassoon homology domains (PBH-domains). It is generally found together with piccolo, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Bassoon was suggested to be a scaffolding element of the presynapse but deletion experiments in mice have shown that bassoon is also involved in synaptic vesicle cycling. Probably bassoon interacts with other protein factors via its Zn²⁺ domain but the potential partners have not been determined yet.

1 ) Dresbach T et al., Mol. Cell. Neurosci. (2003)
2 ) Shapira M et al., Neuron (2003)
3 ) Altrock WD et al., Neuron (2003)
4 ) Dick O et al., Neuron (2003)
5 ) Dick O et al., J. Comp. Neurol. (2001)
6 ) Sanmartí-Vila L et al., Biochem. Biophys. Res. Commun. (2000)
7 ) tom Dieck S et al., J. Cell Biol. (1998)

Protocols

Standard protocol

ICC

IHC-P

ELISA

Membrane proteins

OTHER

Preabsorbtion

Other products for this target

Bassoon antibody - 141 021

References for Bassoon - 141 021