Synaptic Systems

Cat. No.: 141 021

Amount: 200 µl

Price: $365.00

Cat. No. 141 021	200 µl hybridoma supernatant, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 100 up to 1 : 500 gallery IHC: not recommended IHC-P: 1 : 500 gallery ELISA:
Clone	219E1
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat Bassoon. (UniProt Id: O88778)
Reactivity	Reacts with: rat (O88778), mouse (O88737). Other species not tested yet.
Specificity	Specific for Bassoon
Matching control protein/peptide	141-0P
Remarks	WB: Due to the large size of this protein, we recommend NuPAGE 3-8% Tris-Acetate gels for SDS-PAGE. ICC: 2% formaldehyde/PFA fixation is recommended. ELISA: The ELISA-protocol for membrane proteins is required.
Data sheet	141_021.pdf

LAR receptor phospho-tyrosine phosphatases regulate NMDA-receptor responses.
Sclip A, Südhof TC
eLife (2020) 9: . 141 021 WB; tested species: mouse

Microtubule-associated protein 1B (MAP1B)-deficient neurons show structural presynaptic deficiencies in vitro and altered presynaptic physiology.
Bodaleo FJ, Montenegro-Venegas C, Henríquez DR, Court FA, Gonzalez-Billault C
Scientific reports (2016) 6: 30069. 141 021 WB; tested species: mouse

Regulation of presynaptic anchoring of the scaffold protein Bassoon by phosphorylation-dependent interaction with 14-3-3 adaptor proteins.
Schröder MS, Stellmacher A, Romorini S, Marini C, Montenegro-Venegas C, Altrock WD, Gundelfinger ED, Fejtova A
PloS one (2013) 83: e58814. 141 021 WB

Cat. No.: 141 021

Amount: 200 µl

Price: $365.00

ICC

IHC-P

Background

Protocols

Bassoon antibody - 141 021