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Home > Products > 141 021

Bassoon antibody - 141 021

Bassoon is a large multi-domain protein of the presynaptic active zone
Mouse monoclonal hybridoma supernatant
Cat. No.: 141 021
Amount: 200 µl
Price: $365.00
Cat. No. 141 021 200 µl hybridoma supernatant, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC: not recommended
IHC-P: 1 : 500 gallery  
ELISA: yes (see remarks)
Clone 219E1
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat Bassoon. (UniProt Id: O88778)
Reactivity Reacts with: rat (O88778), mouse (O88737).
Other species not tested yet.
Specificity Specific for Bassoon
Matching control protein/peptide 141-0P
Remarks

WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
ICC: Mild fixation conditions (2 % PFA for 20 min) are recommended.
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 141 003 as detector antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet 141_021.pdf

References for Bassoon - 141 021

LAR receptor phospho-tyrosine phosphatases regulate NMDA-receptor responses.
Sclip A, Südhof TC
eLife (2020) 9: . 141 021 WB; tested species: mouse
Microtubule-associated protein 1B (MAP1B)-deficient neurons show structural presynaptic deficiencies in vitro and altered presynaptic physiology.
Bodaleo FJ, Montenegro-Venegas C, Henríquez DR, Court FA, Gonzalez-Billault C
Scientific reports (2016) 6: 30069. 141 021 WB; tested species: mouse
Regulation of presynaptic anchoring of the scaffold protein Bassoon by phosphorylation-dependent interaction with 14-3-3 adaptor proteins.
Schröder MS, Stellmacher A, Romorini S, Marini C, Montenegro-Venegas C, Altrock WD, Gundelfinger ED, Fejtova A
PloS one (2013) 83: e58814. 141 021 WB
Cat. No.: 141 021
Amount: 200 µl
Price: $365.00
WB
ICC
IHC-P
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