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Bassoon antibody - 141 005

Bassoon is a large multi-domain protein of the presynaptic active zone
Guinea pig polyclonal purified antibody
Cat. No.: 141 005
Amount: 50 µg
Price: $450.00
Cat. No. 141 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 (AP-staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: not tested yet
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat Bassoon. (UniProt Id: O88778)
Reactivity Reacts with: rat (O88778), mouse (O88737).
Other species not tested yet.
Specificity Specific for Bassoon
Remarks

WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.

Data sheet 141_005.pdf
Cat. No.: 141 005
Amount: 50 µg
Price: $450.00
Background

Bassoon is a large protein which consists of an N-terminal Zn2+ finger and several piccolo-bassoon homology domains (PBH-domains). It is generally found together with piccolo, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Bassoon was suggested to be a scaffolding element of the presynapse but deletion experiments in mice have shown that bassoon is also involved in synaptic vesicle cycling. Probably bassoon interacts with other protein factors via its Zn2+ domain but the potential partners have not been determined yet.