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Bassoon - 141 003

A large multi-domain protein of the presynaptic active zone
Polyclonal rabbit purified antibody
Cat. No.: 141 003
Amount: 50 µg
Price: $460.00
Cat. No. 141 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 100 up to 1 : 1000 (AP staining) (see remarks)
IP: not tested yet
ICC: 1 : 200 up to 1 : 2000 gallery  
IHC: 1 : 200 gallery  
IHC-P/FFPE: 1 : 500 gallery  
ELISA: yes (see remarks)
Immunogen Recombinant protein corresponding to AA 3569 to 3769 from rat Bassoon (UniProt Id: O88778)
Reactivity Reacts with: rat (O88778), mouse (O88737).
No signal: chicken.
Other species not tested yet.
Specificity Specific for bassoon.
Remarks

WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 141 021 or 141 011 as capture antibody. The ELISA-protocol for membrane proteins is recommended.

Data sheet 141_003.pdf

References for Bassoon - 141 003

Postsynaptic RIM1 modulates synaptic function by facilitating membrane delivery of recycling NMDARs in hippocampal neurons.
Wang J, Lv X, Wu Y, Xu T, Jiao M, Yang R, Li X, Chen M, Yan Y, Chen C, Dong W, et al.
Nature communications (2018) 91: 2267. 141 003 WB; tested species: mouse
OCD-like behavior is caused by dysfunction of thalamo-amygdala circuits and upregulated TrkB/ERK-MAPK signaling as a result of SPRED2 deficiency.
Ullrich M, Weber M, Post AM, Popp S, Grein J, Zechner M, Guerrero González H, Kreis A, Schmitt AG, Üçeyler N, Lesch KP, et al.
Molecular psychiatry (2017) : . 141 003 WB
How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 141 003 WB
Myosin 18Aα targets the guanine nucleotide exchange factor β-Pix to the dendritic spines of cerebellar Purkinje neurons and promotes spine maturation.
Alexander CJ, Barzik M, Fujiwara I, Remmert K, Wang YX, Petralia RS, Friedman TB, Hammer JA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 351: e21092. 141 003 ICC; tested species: mouse
TDP-43 proteinopathy impairs mRNP granule mediated postsynaptic translation and mRNA metabolism.
Wong CE, Jin LW, Chu YP, Wei WY, Ho PC, Tsai KJ
Theranostics (2021) 111: 330-345. 141 003 ICC; tested species: mouse
LOTUS, an endogenous Nogo receptor antagonist, is involved in synapse and memory formation.
Nishida R, Kawaguchi Y, Matsubayashi J, Ishikawa R, Kida S, Takei K
Scientific reports (2021) 111: 5085. 141 003 ICC; tested species: mouse
Deletion of the Nucleotide Exchange Factor Vav3 Enhances Axonal Complexity and Synapse Formation but Tampers Activity of Hippocampal Neuronal Networks In Vitro.
Wegrzyn D, Wegrzyn C, Tedford K, Fischer KD, Faissner A
International journal of molecular sciences (2020) 213: . 141 003 ICC; tested species: mouse
Deletion of LRP1 From Astrocytes Modifies Neuronal Network Activity in an in vitro Model of the Tripartite Synapse.
Romeo R, Glotzbach K, Scheller A, Faissner A
Frontiers in cellular neuroscience (2020) 14: 567253. 141 003 ICC; tested species: mouse
Synaptic FUS Localization During Motoneuron Development and Its Accumulation in Human ALS Synapses.
Deshpande D, Higelin J, Schoen M, Vomhof T, Boeckers TM, Demestre M, Michaelis J
Frontiers in cellular neuroscience (2019) 13: 256. 141 003 ICC; tested species: rat
Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters.
Trotter JH, Hao J, Maxeiner S, Tsetsenis T, Liu Z, Zhuang X, Südhof TC
The Journal of cell biology (2019) : . 141 003 ICC; tested species: mouse
Synaptic nanomodules underlie the organization and plasticity of spine synapses.
Hruska M, Henderson N, Le Marchand SJ, Jafri H, Dalva MB
Nature neuroscience (2018) 215: 671-682. 141 003 ICC; tested species: rat
A machine learning approach for online automated optimization of super-resolution optical microscopy.
Durand A, Wiesner T, Gardner MA, Robitaille LÉ, Bilodeau A, Gagné C, De Koninck P, Lavoie-Cardinal F
Nature communications (2018) 91: 5247. 141 003 ICC; tested species: rat
Paralogs of the Calcium-Dependent Activator Protein for Secretion Differentially Regulate Synaptic Transmission and Peptide Secretion in Sensory Neurons.
Shaib AH, Staudt A, Harb A, Klose M, Shaaban A, Schirra C, Mohrmann R, Rettig J, Becherer U
Frontiers in cellular neuroscience (2018) 12: 304. 141 003 ICC; tested species: mouse
Multicolour Multilevel STED nanoscopy of Actin/Spectrin Organization at Synapses.
Sidenstein SC, D'Este E, Böhm MJ, Danzl JG, Belov VN, Hell SW
Scientific reports (2016) 6: 26725. 141 003 ICC
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 141 003 ICC; tested species: rat
Super-Resolution Microscopy Reveals Presynaptic Localization of the ALS/FTD Related Protein FUS in Hippocampal Neurons.
Schoen M, Reichel JM, Demestre M, Putz S, Deshpande D, Proepper C, Liebau S, Schmeisser MJ, Ludolph AC, Michaelis J, Boeckers TM, et al.
Frontiers in cellular neuroscience (2015) 9: 496. 141 003 ICC; tested species: rat
βCaMKII plays a nonenzymatic role in hippocampal synaptic plasticity and learning by targeting αCaMKII to synapses.
Borgesius NZ, van Woerden GM, Buitendijk GH, Keijzer N, Jaarsma D, Hoogenraad CC, Elgersma Y
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10141-8. 141 003 ICC; tested species: mouse
Calcium binding to PICK1 is essential for the intracellular retention of AMPA receptors underlying long-term depression.
Citri A, Bhattacharyya S, Ma C, Morishita W, Fang S, Rizo J, Malenka RC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3049: 16437-52. 141 003 ICC; tested species: rat
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 141 003 IHC; tested species: rat
Loss of PTEN-induced kinase 1 (Pink1) reduces hippocampal tyrosine hydroxylase and impairs learning and memory.
Maynard ME, Redell JB, Kobori N, Underwood EL, Fischer TD, Hood KN, LaRoche V, Waxham MN, Moore AN, Dash PK
Experimental neurology (2019) 323: 113081. 141 003 IHC; tested species: mouse
Superresolution fluorescence microscopy for 3D reconstruction of thick samples.
Park S, Kang W, Kwon YD, Shim J, Kim S, Kaang BK, Hohng S
Molecular brain (2018) 111: 17. 141 003 IHC; tested species: mouse
Tissue multicolor STED nanoscopy of presynaptic proteins in the calyx of Held.
Kempf C, Staudt T, Bingen P, Horstmann H, Engelhardt J, Hell SW, Kuner T
PloS one (2013) 84: e62893. 141 003 IHC; tested species: rat
Expression of Ttyh1, a member of the Tweety family in neurons in vitro and in vivo and its potential role in brain pathology.
Stefaniuk M, Swiech L, Dzwonek J, Lukasiuk K
Journal of neurochemistry (2010) 1155: 1183-94. 141 003 IHC; tested species: rat
Superresolution imaging of chemical synapses in the brain.
Dani A, Huang B, Bergan J, Dulac C, Zhuang X
Neuron (2010) 685: 843-56. 141 003 IHC; tested species: mouse
Cat. No.: 141 003
Quantity: 50 µg
Price: $460.00
Postsynaptic RIM1 modulates synaptic function by facilitating membrane delivery of recycling NMDARs in hippocampal neurons.
Wang J, Lv X, Wu Y, Xu T, Jiao M, Yang R, Li X, Chen M, Yan Y, Chen C, Dong W, et al.
Nature communications (2018) 91: 2267. 141 003 WB; tested species: mouse
OCD-like behavior is caused by dysfunction of thalamo-amygdala circuits and upregulated TrkB/ERK-MAPK signaling as a result of SPRED2 deficiency.
Ullrich M, Weber M, Post AM, Popp S, Grein J, Zechner M, Guerrero González H, Kreis A, Schmitt AG, Üçeyler N, Lesch KP, et al.
Molecular psychiatry (2017) : . 141 003 WB
How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 141 003 WB
Myosin 18Aα targets the guanine nucleotide exchange factor β-Pix to the dendritic spines of cerebellar Purkinje neurons and promotes spine maturation.
Alexander CJ, Barzik M, Fujiwara I, Remmert K, Wang YX, Petralia RS, Friedman TB, Hammer JA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 351: e21092. 141 003 ICC; tested species: mouse
TDP-43 proteinopathy impairs mRNP granule mediated postsynaptic translation and mRNA metabolism.
Wong CE, Jin LW, Chu YP, Wei WY, Ho PC, Tsai KJ
Theranostics (2021) 111: 330-345. 141 003 ICC; tested species: mouse
LOTUS, an endogenous Nogo receptor antagonist, is involved in synapse and memory formation.
Nishida R, Kawaguchi Y, Matsubayashi J, Ishikawa R, Kida S, Takei K
Scientific reports (2021) 111: 5085. 141 003 ICC; tested species: mouse
Deletion of the Nucleotide Exchange Factor Vav3 Enhances Axonal Complexity and Synapse Formation but Tampers Activity of Hippocampal Neuronal Networks In Vitro.
Wegrzyn D, Wegrzyn C, Tedford K, Fischer KD, Faissner A
International journal of molecular sciences (2020) 213: . 141 003 ICC; tested species: mouse
Deletion of LRP1 From Astrocytes Modifies Neuronal Network Activity in an in vitro Model of the Tripartite Synapse.
Romeo R, Glotzbach K, Scheller A, Faissner A
Frontiers in cellular neuroscience (2020) 14: 567253. 141 003 ICC; tested species: mouse
Synaptic FUS Localization During Motoneuron Development and Its Accumulation in Human ALS Synapses.
Deshpande D, Higelin J, Schoen M, Vomhof T, Boeckers TM, Demestre M, Michaelis J
Frontiers in cellular neuroscience (2019) 13: 256. 141 003 ICC; tested species: rat
Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters.
Trotter JH, Hao J, Maxeiner S, Tsetsenis T, Liu Z, Zhuang X, Südhof TC
The Journal of cell biology (2019) : . 141 003 ICC; tested species: mouse
Synaptic nanomodules underlie the organization and plasticity of spine synapses.
Hruska M, Henderson N, Le Marchand SJ, Jafri H, Dalva MB
Nature neuroscience (2018) 215: 671-682. 141 003 ICC; tested species: rat
A machine learning approach for online automated optimization of super-resolution optical microscopy.
Durand A, Wiesner T, Gardner MA, Robitaille LÉ, Bilodeau A, Gagné C, De Koninck P, Lavoie-Cardinal F
Nature communications (2018) 91: 5247. 141 003 ICC; tested species: rat
Paralogs of the Calcium-Dependent Activator Protein for Secretion Differentially Regulate Synaptic Transmission and Peptide Secretion in Sensory Neurons.
Shaib AH, Staudt A, Harb A, Klose M, Shaaban A, Schirra C, Mohrmann R, Rettig J, Becherer U
Frontiers in cellular neuroscience (2018) 12: 304. 141 003 ICC; tested species: mouse
Multicolour Multilevel STED nanoscopy of Actin/Spectrin Organization at Synapses.
Sidenstein SC, D'Este E, Böhm MJ, Danzl JG, Belov VN, Hell SW
Scientific reports (2016) 6: 26725. 141 003 ICC
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 141 003 ICC; tested species: rat
Super-Resolution Microscopy Reveals Presynaptic Localization of the ALS/FTD Related Protein FUS in Hippocampal Neurons.
Schoen M, Reichel JM, Demestre M, Putz S, Deshpande D, Proepper C, Liebau S, Schmeisser MJ, Ludolph AC, Michaelis J, Boeckers TM, et al.
Frontiers in cellular neuroscience (2015) 9: 496. 141 003 ICC; tested species: rat
βCaMKII plays a nonenzymatic role in hippocampal synaptic plasticity and learning by targeting αCaMKII to synapses.
Borgesius NZ, van Woerden GM, Buitendijk GH, Keijzer N, Jaarsma D, Hoogenraad CC, Elgersma Y
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10141-8. 141 003 ICC; tested species: mouse
Calcium binding to PICK1 is essential for the intracellular retention of AMPA receptors underlying long-term depression.
Citri A, Bhattacharyya S, Ma C, Morishita W, Fang S, Rizo J, Malenka RC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3049: 16437-52. 141 003 ICC; tested species: rat
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 141 003 IHC; tested species: rat
Loss of PTEN-induced kinase 1 (Pink1) reduces hippocampal tyrosine hydroxylase and impairs learning and memory.
Maynard ME, Redell JB, Kobori N, Underwood EL, Fischer TD, Hood KN, LaRoche V, Waxham MN, Moore AN, Dash PK
Experimental neurology (2019) 323: 113081. 141 003 IHC; tested species: mouse
Superresolution fluorescence microscopy for 3D reconstruction of thick samples.
Park S, Kang W, Kwon YD, Shim J, Kim S, Kaang BK, Hohng S
Molecular brain (2018) 111: 17. 141 003 IHC; tested species: mouse
Tissue multicolor STED nanoscopy of presynaptic proteins in the calyx of Held.
Kempf C, Staudt T, Bingen P, Horstmann H, Engelhardt J, Hell SW, Kuner T
PloS one (2013) 84: e62893. 141 003 IHC; tested species: rat
Expression of Ttyh1, a member of the Tweety family in neurons in vitro and in vivo and its potential role in brain pathology.
Stefaniuk M, Swiech L, Dzwonek J, Lukasiuk K
Journal of neurochemistry (2010) 1155: 1183-94. 141 003 IHC; tested species: rat
Superresolution imaging of chemical synapses in the brain.
Dani A, Huang B, Bergan J, Dulac C, Zhuang X
Neuron (2010) 685: 843-56. 141 003 IHC; tested species: mouse
Background

Bassoon is a large protein which consists of an N-terminal Zn2+ finger and several piccolo-bassoon homology domains (PBH-domains). It is generally found together with piccolo, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Bassoon was suggested to be a scaffolding element of the presynapse but deletion experiments in mice have shown that bassoon is also involved in synaptic vesicle cycling. Probably bassoon interacts with other protein factors via its Zn2+ domain but the potential partners have not been determined yet.