Synaptic Systems

Cat. No.: 141 003

Amount: 50 µg

Price: $470.00

Cat. No. 141 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 100 up to 1 : 1000 (AP staining) (see remarks) IP: not tested yet ICC: 1 : 200 up to 1 : 2000 gallery IHC: 1 : 200 gallery IHC-P: 1 : 500 gallery ExM: 1 : 400 (see remarks) gallery ELISA: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat Bassoon. (UniProt Id: O88778)
Reactivity	Reacts with: rat (O88778), mouse (O88737). No signal: chicken. Other species not tested yet.
Specificity	Specific for Bassoon
Remarks	WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen. ExM: This antibody has been successfully used for the epitope-preserving magnified analysis of the proteome (eMAP) expansion microscopy method (Park et al. 2021. PMID: 34767453). ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 141 021 or 141 011 as capture antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet	141_003.pdf

References for Bassoon - 141 003

Postsynaptic RIM1 modulates synaptic function by facilitating membrane delivery of recycling NMDARs in hippocampal neurons.
Wang J, Lv X, Wu Y, Xu T, Jiao M, Yang R, Li X, Chen M, Yan Y, Chen C, Dong W, et al.
Nature communications (2018) 91: 2267. 141 003 WB; tested species: mouse

OCD-like behavior is caused by dysfunction of thalamo-amygdala circuits and upregulated TrkB/ERK-MAPK signaling as a result of SPRED2 deficiency.
Ullrich M, Weber M, Post AM, Popp S, Grein J, Zechner M, Guerrero González H, Kreis A, Schmitt AG, Üçeyler N, Lesch KP, et al.
Molecular psychiatry (2017) : . 141 003 WB

How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 141 003 WB

Astrocytic TDP-43 dysregulation impairs memory by modulating antiviral pathways and interferon-inducible chemokines.
Licht-Murava A, Meadows SM, Palaguachi F, Song SC, Jackvony S, Bram Y, Zhou C, Schwartz RE, Froemke RC, Orr AL, Orr AG, et al.
Science advances (2023) 916: eade1282. 141 003 ICC; tested species: mouse

Microglia-synapse engulfment via PtdSer-TREM2 ameliorates neuronal hyperactivity in Alzheimer's disease models.
Rueda-Carrasco J, Sokolova D, Lee SE, Childs T, Jurčáková N, Crowley G, De Schepper S, Ge JZ, Lachica JI, Toomey CE, Freeman OJ, et al.
The EMBO journal (2023) 4219: e113246. 141 003 ICC; tested species: rat

Filamin A organizes γ‑aminobutyric acid type B receptors at the plasma membrane.
Jobin ML, Siddig S, Koszegi Z, Lanoiselée Y, Khayenko V, Sungkaworn T, Werner C, Seier K, Misigaiski C, Mantovani G, Sauer M, et al.
Nature communications (2023) 141: 34. 141 003 ExM, ICC; tested species: mouse

α-Synuclein induced cholesterol lowering increases tonic and reduces depolarization-evoked synaptic vesicle recycling and glutamate release.
Lazarevic V, Yang Y, Paslawski W, Svenningsson P
NPJ Parkinson's disease (2022) 81: 71. 141 003 ICC; tested species: mouse

SRF depletion in early life contributes to social interaction deficits in the adulthood.
Roszkowska M, Krysiak A, Majchrowicz L, Nader K, Beroun A, Michaluk P, Pekala M, Jaworski J, Kondrakiewicz L, Puścian A, Knapska E, et al.
Cellular and molecular life sciences : CMLS (2022) 795: 278. 141 003 ICC; tested species: rat

Nanoscale rules governing the organization of glutamate receptors in spine synapses are subunit specific.
Hruska M, Cain RE, Dalva MB
Nature communications (2022) 131: 920. 141 003 ICC; tested species: rat

Myosin 18Aα targets the guanine nucleotide exchange factor β-Pix to the dendritic spines of cerebellar Purkinje neurons and promotes spine maturation.
Alexander CJ, Barzik M, Fujiwara I, Remmert K, Wang YX, Petralia RS, Friedman TB, Hammer JA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 351: e21092. 141 003 ICC; tested species: mouse

AMPA receptor anchoring at CA1 synapses is determined by N-terminal domain and TARP γ8 interactions.
Watson JF, Pinggera A, Ho H, Greger IH
Nature communications (2021) 121: 5083. 141 003 ICC; tested species: mouse

SHANK2 Mutations Result in Dysregulation of the ERK1/2 Pathway in Human Induced Pluripotent Stem Cells-Derived Neurons and Shank2(-/-) Mice.
Lutz AK, Pérez Arévalo A, Ioannidis V, Stirmlinger N, Demestre M, Delorme R, Bourgeron T, Boeckers TM
Frontiers in molecular neuroscience (2021) 14: 773571. 141 003 ICC; tested species: human

LOTUS, an endogenous Nogo receptor antagonist, is involved in synapse and memory formation.
Nishida R, Kawaguchi Y, Matsubayashi J, Ishikawa R, Kida S, Takei K
Scientific reports (2021) 111: 5085. 141 003 ICC; tested species: mouse

TDP-43 proteinopathy impairs mRNP granule mediated postsynaptic translation and mRNA metabolism.
Wong CE, Jin LW, Chu YP, Wei WY, Ho PC, Tsai KJ
Theranostics (2021) 111: 330-345. 141 003 ICC; tested species: mouse

Deletion of the Nucleotide Exchange Factor Vav3 Enhances Axonal Complexity and Synapse Formation but Tampers Activity of Hippocampal Neuronal Networks In Vitro.
Wegrzyn D, Wegrzyn C, Tedford K, Fischer KD, Faissner A
International journal of molecular sciences (2020) 213: . 141 003 ICC; tested species: mouse

Deletion of LRP1 From Astrocytes Modifies Neuronal Network Activity in an in vitro Model of the Tripartite Synapse.
Romeo R, Glotzbach K, Scheller A, Faissner A
Frontiers in cellular neuroscience (2020) 14: 567253. 141 003 ICC; tested species: mouse

Synaptic FUS Localization During Motoneuron Development and Its Accumulation in Human ALS Synapses.
Deshpande D, Higelin J, Schoen M, Vomhof T, Boeckers TM, Demestre M, Michaelis J
Frontiers in cellular neuroscience (2019) 13: 256. 141 003 ICC; tested species: rat

Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters.
Trotter JH, Hao J, Maxeiner S, Tsetsenis T, Liu Z, Zhuang X, Südhof TC
The Journal of cell biology (2019) : . 141 003 ICC; tested species: mouse

Paralogs of the Calcium-Dependent Activator Protein for Secretion Differentially Regulate Synaptic Transmission and Peptide Secretion in Sensory Neurons.
Shaib AH, Staudt A, Harb A, Klose M, Shaaban A, Schirra C, Mohrmann R, Rettig J, Becherer U
Frontiers in cellular neuroscience (2018) 12: 304. 141 003 ICC; tested species: mouse

A machine learning approach for online automated optimization of super-resolution optical microscopy.
Durand A, Wiesner T, Gardner MA, Robitaille LÉ, Bilodeau A, Gagné C, De Koninck P, Lavoie-Cardinal F
Nature communications (2018) 91: 5247. 141 003 ICC; tested species: rat

Synaptic nanomodules underlie the organization and plasticity of spine synapses.
Hruska M, Henderson N, Le Marchand SJ, Jafri H, Dalva MB
Nature neuroscience (2018) 215: 671-682. 141 003 ICC; tested species: rat

Multicolour Multilevel STED nanoscopy of Actin/Spectrin Organization at Synapses.
Sidenstein SC, D'Este E, Böhm MJ, Danzl JG, Belov VN, Hell SW
Scientific reports (2016) 6: 26725. 141 003 ICC

Super-Resolution Microscopy Reveals Presynaptic Localization of the ALS/FTD Related Protein FUS in Hippocampal Neurons.
Schoen M, Reichel JM, Demestre M, Putz S, Deshpande D, Proepper C, Liebau S, Schmeisser MJ, Ludolph AC, Michaelis J, Boeckers TM, et al.
Frontiers in cellular neuroscience (2015) 9: 496. 141 003 ICC; tested species: rat

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 141 003 ICC; tested species: rat

βCaMKII plays a nonenzymatic role in hippocampal synaptic plasticity and learning by targeting αCaMKII to synapses.
Borgesius NZ, van Woerden GM, Buitendijk GH, Keijzer N, Jaarsma D, Hoogenraad CC, Elgersma Y
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10141-8. 141 003 ICC; tested species: mouse

Calcium binding to PICK1 is essential for the intracellular retention of AMPA receptors underlying long-term depression.
Citri A, Bhattacharyya S, Ma C, Morishita W, Fang S, Rizo J, Malenka RC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3049: 16437-52. 141 003 ICC; tested species: rat

Microglial dyshomeostasis drives perineuronal net and synaptic loss in a CSF1R+/- mouse model of ALSP, which can be rescued via CSF1R inhibitors.
Arreola MA, Soni N, Crapser JD, Hohsfield LA, Elmore MRP, Matheos DP, Wood MA, Swarup V, Mortazavi A, Green KN
Science advances (2021) 735: . 141 003 IHC; tested species: mouse

A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 141 003 IHC; tested species: rat

Loss of PTEN-induced kinase 1 (Pink1) reduces hippocampal tyrosine hydroxylase and impairs learning and memory.
Maynard ME, Redell JB, Kobori N, Underwood EL, Fischer TD, Hood KN, LaRoche V, Waxham MN, Moore AN, Dash PK
Experimental neurology (2019) 323: 113081. 141 003 IHC; tested species: mouse

Superresolution fluorescence microscopy for 3D reconstruction of thick samples.
Park S, Kang W, Kwon YD, Shim J, Kim S, Kaang BK, Hohng S
Molecular brain (2018) 111: 17. 141 003 IHC; tested species: mouse

Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues.
Ku T, Swaney J, Park JY, Albanese A, Murray E, Cho JH, Park YG, Mangena V, Chen J, Chung K
Nature biotechnology (2016) 349: 973-81. 141 003 IHC; tested species: mouse

Tissue multicolor STED nanoscopy of presynaptic proteins in the calyx of Held.
Kempf C, Staudt T, Bingen P, Horstmann H, Engelhardt J, Hell SW, Kuner T
PloS one (2013) 84: e62893. 141 003 IHC; tested species: rat

Expression of Ttyh1, a member of the Tweety family in neurons in vitro and in vivo and its potential role in brain pathology.
Stefaniuk M, Swiech L, Dzwonek J, Lukasiuk K
Journal of neurochemistry (2010) 1155: 1183-94. 141 003 IHC; tested species: rat

Superresolution imaging of chemical synapses in the brain.
Dani A, Huang B, Bergan J, Dulac C, Zhuang X
Neuron (2010) 685: 843-56. 141 003 IHC; tested species: mouse

Epitope-preserving magnified analysis of proteome (eMAP).
Park J, Khan S, Yun DH, Ku T, Villa KL, Lee JE, Zhang Q, Park J, Feng G, Nedivi E, Chung K, et al.
Science advances (2021) 746: eabf6589. 141 003 Clarity; tested species: mouse,marmoset

Cat. No.: 141 003

Amount: 50 µg

Price: $470.00

How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 141 003 WB

Multicolour Multilevel STED nanoscopy of Actin/Spectrin Organization at Synapses.
Sidenstein SC, D'Este E, Böhm MJ, Danzl JG, Belov VN, Hell SW
Scientific reports (2016) 6: 26725. 141 003 ICC

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 141 003 ICC; tested species: rat

Superresolution imaging of chemical synapses in the brain.
Dani A, Huang B, Bergan J, Dulac C, Zhuang X
Neuron (2010) 685: 843-56. 141 003 IHC; tested species: mouse

ICC

IHC

IHC-P

ExM

Background

Bassoon is a large protein which consists of an N-terminal Zn²⁺ finger and several piccolo-bassoon homology domains (PBH-domains). It is generally found together with piccolo, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Bassoon was suggested to be a scaffolding element of the presynapse but deletion experiments in mice have shown that bassoon is also involved in synaptic vesicle cycling. Probably bassoon interacts with other protein factors via its Zn²⁺ domain but the potential partners have not been determined yet.

1 ) Dresbach T et al., Mol. Cell. Neurosci. (2003)
2 ) Shapira M et al., Neuron (2003)
3 ) Altrock WD et al., Neuron (2003)
4 ) Dick O et al., Neuron (2003)
5 ) Dick O et al., J. Comp. Neurol. (2001)
6 ) Sanmartí-Vila L et al., Biochem. Biophys. Res. Commun. (2000)
7 ) tom Dieck S et al., J. Cell Biol. (1998)

Protocols

ICC

IHC

IHC-P

ELISA

Membrane proteins

Other products for this target

Bassoon antibody - 141 003

References for Bassoon - 141 003