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Home > Products > 141 003

Bassoon antibody - 141 003

Bassoon is a large multi-domain protein of the presynaptic active zone
Rabbit polyclonal purified antibody
Cat. No.: 141 003
Amount: 50 µg
Price: $475.00
Cat. No. 141 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 1000 (AP staining) (see remarks)
IP: not tested yet
ICC: 1 : 200 up to 1 : 2000 gallery  
IHC: 1 : 200 gallery  
IHC-P: 1 : 500 gallery  
ExM: 1 : 400 (see remarks) gallery  
ELISA: yes (see remarks)
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat Bassoon. (UniProt Id: O88778)
Reactivity Reacts with: rat (O88778), mouse (O88737).
No signal: chicken.
Other species not tested yet.
Specificity Specific for Bassoon
Remarks

WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
ExM: This antibody has been successfully used for the epitope-preserving magnified analysis of the proteome (eMAP) expansion microscopy method (Park et al. 2021. PMID: 34767453).
ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 141 021 or 141 011 as capture antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet 141_003.pdf

References for Bassoon - 141 003

Postsynaptic RIM1 modulates synaptic function by facilitating membrane delivery of recycling NMDARs in hippocampal neurons.
Wang J, Lv X, Wu Y, Xu T, Jiao M, Yang R, Li X, Chen M, Yan Y, Chen C, Dong W, et al.
Nature communications (2018) 91: 2267. 141 003 WB; tested species: mouse
OCD-like behavior is caused by dysfunction of thalamo-amygdala circuits and upregulated TrkB/ERK-MAPK signaling as a result of SPRED2 deficiency.
Ullrich M, Weber M, Post AM, Popp S, Grein J, Zechner M, Guerrero González H, Kreis A, Schmitt AG, Üçeyler N, Lesch KP, et al.
Molecular psychiatry (2017) : . 141 003 WB
How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 141 003 WB
Cat. No.: 141 003
Amount: 50 µg
Price: $475.00
ICC
IHC
IHC-P
ExM
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