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Rim 1/2 antibody - 140 217

RIMs are Rab3 effector proteins of the cytomatrix at the active zone
Rat monoclonal purified IgG
Cat. No.: 140 217
Amount: 100 µg
Price: $415.00
Cat. No. 140 217 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 100 (see remarks) gallery  
IHC-P/FFPE: not tested yet
Clone SY-53E12
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to Zn-finger-domain of rat RIM 2. (UniProt Id: Q9JIS1)
Reactivity Reacts with: mouse (Q99NE5, Q9EQZ7), rat (Q9JIS1, Q9JIR4).
Other species not tested yet.
Specificity RIM 2 including splice variants, cross reacts to RIM 1
Remarks

IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical stainings.

Data sheet 140_217.pdf
Cat. No.: 140 217
Amount: 100 µg
Price: $415.00
Background

RIMs are presynaptic active zone proteins that regulate Ca2+ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 1α is a putative Rab 3a effector and has been shown to interact with other active zone proteins like Munc13-1, ERC 1b, ERC 2 and α-liprins. Deletion of RIM 1α in mice impaired neurotransmitter release without changing the structure of the synapse.
RIM 2β consists of a specific N-terminus, the central PDZ domain and the C-terminal C2 domains. The mRNA for RIM 2β is transcribed from an internal promoter of the RIM 2α gene.
Shorter variants of RIM 2 which comprise only the C-terminal C2B domain and some flanking regions are referred to as NIM 2 / RIM 2γ and NIM 3 / RIM 3γ.