Synaptic Systems

Cat. No.: 140 213

Amount: 50 µg

Price: $380.00

Cat. No. 140 213	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 466 from rat Rim2 (UniProt Id: Q9JIS1)
Reactivity	Reacts with: rat (Q9JIS1, Q9JIR4), mouse (Q99NE5, Q9EQZ7). Other species not tested yet.
Specificity	RIM 2 including splice variants, cross reacts to RIM 1.
Data sheet	Datasheet 140_213

References for RIM1/2 - 140 213

Munc13-1 restoration mitigates presynaptic pathology in spinal muscular atrophy.
Moradi M, Weingart J, Deng C, Nasouti M, Briese M, Jablonka S, Sauer M, Sendtner M
Nature communications (2025) 161: 8724. 140 213 ICC; tested species: mouse

Loss of synaptic Munc13-1 underlies neurotransmission abnormalities in spinal muscular atrophy.
Moradi M, Deng C, Sendtner M
Cellular and molecular life sciences : CMLS (2025) 821: 325. 140 213 ICC; tested species: mouse

Structural and functional reorganization of inhibitory synapses by activity-dependent cleavage of neuroligin-2.
Xu N, Cao R, Chen SY, Gou XZ, Wang B, Luo HM, Gao F, Tang AH
Proceedings of the National Academy of Sciences of the United States of America (2024) 12118: e2314541121. 140 213 ICC; tested species: mouse

LRRTM2 controls presynapse nano-organization and AMPA receptor sub-positioning through Neurexin-binding interface.
Liouta K, Lubas M, Venugopal V, Chabbert J, Jeannière C, Diaz C, Munier M, Tessier B, Claverol S, Favereaux A, Sainlos M, et al.
Nature communications (2024) 151: 8807. 140 213 ICC; tested species: mouse

Cat. No.: 140 213

Amount: 50 µg

Price: $380.00

ICC

Background

RIMs are presynaptic active zone proteins that regulate Ca²⁺ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 1α is a putative Rab 3a effector and has been shown to interact with other active zone proteins like Munc13-1, ERC 1b, ERC 2 and α-liprins. Deletion of RIM 1α in mice impaired neurotransmitter release without changing the structure of the synapse.
RIM 2β consists of a specific N-terminus, the central PDZ domain and the C-terminal C2 domains. The mRNA for RIM 2β is transcribed from an internal promoter of the RIM 2α gene.
Shorter variants of RIM 2 which comprise only the C-terminal C₂B domain and some flanking regions are referred to as NIM 2 / RIM 2γ and NIM 3 / RIM 3γ.

Protocols

ICC

Fixed Cells

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RIM1/2 antibody SNARE binding domain - 140 213

References for RIM1/2 - 140 213