Rim1/2 antibody - 140 208 K.O.

RIMs are Rab3 effector proteins of the cytomatrix at the active zone

Rabbit monoclonal recombinant IgG

Cat. No.: 140 208

Amount: 50 µg

Price: $420.00

Cat. No. 140 208	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 (see remarks) gallery IHC-P (FFPE): not recommended EM: external data (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Clone	Rb53E12
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to Zn-finger-domain of rat RIM 2. (UniProt Id: Q9JIS1)
Reactivity	Reacts with: mouse (Q99NE5, Q9EQZ7), rat (Q9JIS1, Q9JIR4). Other species not tested yet.
Specificity	K.O. validated
Remarks	This antibody is a chimeric antibody based on the monoclonal rat antibody SY-53E12. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. IHC: Antigen retrieval with citrate buffer pH 6 is required. EM: This antibody has been successfully applied and published for this method by customers (see application-specific references).
Data sheet	Datasheet 140_208

References for Rim1/2 - 140 208

Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons.
Kim O, Okamoto Y, Kaufmann WA, Brose N, Shigemoto R, Jonas P
PLoS biology (2024) 2211: e3002879. 140 208 EM; tested species: mouse,rat

Cat. No.: 140 208

Amount: 50 µg

Price: $420.00

ICC

IHC

Background

RIMs are presynaptic active zone proteins that regulate Ca²⁺ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 1α is a putative Rab 3a effector and has been shown to interact with other active zone proteins like Munc13-1, ERC 1b, ERC 2 and α-liprins. Deletion of RIM 1α in mice impaired neurotransmitter release without changing the structure of the synapse.
RIM 2β consists of a specific N-terminus, the central PDZ domain and the C-terminal C2 domains. The mRNA for RIM 2β is transcribed from an internal promoter of the RIM 2α gene.
Shorter variants of RIM 2 which comprise only the C-terminal C₂B domain and some flanking regions are referred to as NIM 2 / RIM 2γ and NIM 3 / RIM 3γ.

Protocols

ICC

Fixed Cells

IHC

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