Tailor-made Antibodies
and Tools for Life Science

VAMP4 antibody - 136 002 K.O.

VAMP4 is a v-SNARE involved in the maturation of secretory granules
Rabbit polyclonal antiserum
Cat. No.: 136 002
Amount: 200 µl
Price: $360.00
Cat. No. 136 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: yes
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 1 to 117 from rat VAMP4 (UniProt Id: D4A560)
Reactivity Reacts with: human (O75379), rat (D4A560), hamster, zebrafish.
Other species not tested yet.
Specificity K.O. validated PubMed: 33931449
Data sheet 136_002.pdf

References for VAMP4 - 136 002

Pleiotropic effects of Syntaxin16 identified by gene editing in cultured adipocytes.
Bremner SK, Al Shammari WS, Milligan RS, Hudson BD, Sutherland C, Bryant NJ, Gould GW
Frontiers in cell and developmental biology (2022) 10: 1033501. 136 002 WB; tested species: rat
Shifting proteomes: limitations in using the BioID proximity labeling system to study SNARE protein trafficking during infection with intracellular pathogens.
Jorgenson LM, Olson-Wood MG, Rucks EA
Pathogens and disease (2021) 797: . 136 002 WB; tested species: human
The vSNAREs VAMP2 and VAMP4 control recycling and intracellular sorting of post-synaptic receptors in neuronal dendrites.
Bakr M, Jullié D, Krapivkina J, Paget-Blanc V, Bouit L, Petersen JD, Retailleau N, Breillat C, Herzog E, Choquet D, Perrais D, et al.
Cell reports (2021) 3610: 109678. 136 002 WB, ICC, EM; KD verified; tested species: rat
VAMP4 maintains a Ca2+-sensitive pool of spontaneously recycling synaptic vesicles.
Lin PY, Chanaday NL, Horvath PM, Ramirez DMO, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 136 002 WB; KD verified; tested species: rat
ELKS1 Captures Rab6-Marked Vesicular Cargo in Presynaptic Nerve Terminals.
Nyitrai H, Wang SSH, Kaeser PS
Cell reports (2020) 3110: 107712. 136 002 WB; tested species: mouse
Characterisation of GLUT4 trafficking in HeLa cells: comparable kinetics and orthologous trafficking mechanisms to 3T3-L1 adipocytes.
Morris S, Geoghegan ND, Sadler JBA, Koester AM, Black HL, Laub M, Miller L, Heffernan L, Simpson JC, Mastick CC, Cooper J, et al.
PeerJ (2020) 8: e8751. 136 002 WB; KD verified; tested species: mouse
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 136 002 WB; tested species: mouse
β1-integrin- and KV1.3 channel-dependent signaling stimulates glutamate release from Th17 cells.
Birkner K, Wasser B, Ruck T, Thalman C, Luchtman D, Pape K, Schmaul S, Bitar L, Krämer-Albers EM, Stroh A, Meuth SG, et al.
The Journal of clinical investigation (2019) : . 136 002 WB, ICC; tested species: mouse
Delayed recruiting of TPD52 to lipid droplets - evidence for a "second wave" of lipid droplet-associated proteins that respond to altered lipid storage induced by Brefeldin A treatment.
Chen Y, Frost S, Khushi M, Cantrill LC, Yu H, Arthur JW, Bright RK, Groblewski GE, Byrne JA
Scientific reports (2019) 91: 9790. 136 002 WB; tested species: human
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications (2019) 101: 1608. 136 002 WB; tested species: human
The phosphatase PTPRG controls FGFR1 activity and influences sensitivity to FGFR kinase inhibitors.
Kostas M, Haugsten EM, Zhen Y, Sorensen V, Szybowska P, Fiorito E, Lorenz S, de Souza GA, Wiedlocha A, Wesche J
Molecular & cellular proteomics : MCP (2018) : . 136 002 WB; tested species: human
VAMP2 is implicated in the secretion of antibodies by human plasma cells and can be replaced by other synaptobrevins.
Gómez-Jaramillo L, Romero-García R, Jiménez-Gómez G, Riegle L, Ramos-Amaya AB, Brieva JA, Kelly-Worden M, Campos-Caro A
Cellular & molecular immunology (2018) 154: 353-366. 136 002 WB, ICC; tested species: human
Identification of a Botulinum Neurotoxin-like Toxin in a Commensal Strain of Enterococcus faecium.
Zhang S, Lebreton F, Mansfield MJ, Miyashita SI, Zhang J, Schwartzman JA, Tao L, Masuyer G, Martínez-Carranza M, Stenmark P, Gilmore MS, et al.
Cell host & microbe (2018) 232: 169-176.e6. 136 002 WB; tested species: mouse
Probing and manipulating intracellular membrane traffic by microinjection of artificial vesicles.
Koike S, Jahn R
Proceedings of the National Academy of Sciences of the United States of America (2017) 11446: E9883-E9892. 136 002 WB; tested species: human
Identification and characterization of a novel botulinum neurotoxin.
Zhang S, Masuyer G, Zhang J, Shen Y, Lundin D, Henriksson L, Miyashita SI, Martínez-Carranza M, Dong M, Stenmark P
Nature communications (2017) 8: 14130. 136 002 WB; tested species: mouse
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 136 002 WB
Annexin A6 and Late Endosomal Cholesterol Modulate Integrin Recycling and Cell Migration.
García-Melero A, Reverter M, Hoque M, Meneses-Salas E, Koese M, Conway JR, Johnsen CH, Alvarez-Guaita A, Morales-Paytuvi F, Elmaghrabi YA, Pol A, et al.
The Journal of biological chemistry (2016) 2913: 1320-35. 136 002 WB, ICC
Selected SNARE proteins are essential for the polarized membrane insertion of igf-1 receptor and the regulation of initial axonal outgrowth in neurons.
Grassi D, Plonka FB, Oksdath M, Guil AN, Sosa LJ, Quiroga S
Cell discovery (2015) 1: 15023. 136 002 WB, ICC
Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking.
Sadler JB, Bryant NJ, Gould GW
Molecular biology of the cell (2015) 263: 530-6. 136 002 WB
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 136 002 WB, ICC, IHC; tested species: mouse,rat
Sorting of GLUT4 into its insulin-sensitive store requires the Sec1/Munc18 protein mVps45.
Roccisana J, Sadler JB, Bryant NJ, Gould GW
Molecular biology of the cell (2013) 2415: 2389-97. 136 002 WB
VAMP4 directs synaptic vesicles to a pool that selectively maintains asynchronous neurotransmission.
Raingo J, Khvotchev M, Liu P, Darios F, Li YC, Ramirez DM, Adachi M, Lemieux P, Toth K, Davletov B, Kavalali ET, et al.
Nature neuroscience (2012) 155: 738-45. 136 002 WB, ICC, IHC
Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport.
Steuble M, Diep TM, Schätzle P, Ludwig A, Tagaya M, Kunz B, Sonderegger P
Biology open (2012) 18: 761-74. 136 002 WB
The COG complex interacts directly with Syntaxin 6 and positively regulates endosome-to-TGN retrograde transport.
Laufman O, Hong W, Lev S
The Journal of cell biology (2011) 1943: 459-72. 136 002 WB, ICC
Dual roles of the mammalian GARP complex in tethering and SNARE complex assembly at the trans-golgi network.
Pérez-Victoria FJ, Bonifacino JS
Molecular and cellular biology (2009) 2919: 5251-63. 136 002 WB
The regulated exocytosis of enlargeosomes is mediated by a SNARE machinery that includes VAMP4.
Cocucci E, Racchetti G, Rupnik M, Meldolesi J
Journal of cell science (2008) 121Pt 18: 2983-91. 136 002 WB, ICC
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 136 002 WB
MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking.
Nakamura N, Fukuda H, Kato A, Hirose S
Molecular biology of the cell (2005) 164: 1696-710. 136 002 WB
Association of gamma-secretase with lipid rafts in post-Golgi and endosome membranes.
Vetrivel KS, Cheng H, Lin W, Sakurai T, Li T, Nukina N, Wong PC, Xu H, Thinakaran G
The Journal of biological chemistry (2004) 27943: 44945-54. 136 002 WB
Cat. No.: 136 002
Amount: 200 µl
Price: $360.00