Cat. No.: 135 421C5
Amount: 100 µg
Price:
$465.00
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| Cat. No. 135 421C5 |
100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 5.
Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.
(Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications |
IP: N/A ICC: not recommended (see remarks) IHC: 1 : 200 up to 1 : 500 gallery IHC-P: not tested yet |
| Label | Sulfo-Cyanine 5 |
| Clone | 95E11 |
| Subtype | IgG2a (κ light chain) |
| Immunogen | Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT2 (UniProt Id: Q9JI12) |
| Reactivity |
Reacts with: rat (Q9JI12), mouse (Q8BLE7). Other species not tested yet. |
| Matching control protein/peptide | 135-4P |
| Remarks |
ICC: As a Sulfo-Cyanine 5 conjugate this antibody produces some unspecifc nuclear staining in cultured primary neurons. |
| Data sheet | 135_421c5.pdf |
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The vesicular glutamate transporter 2 VGLUT 2, also referred to as DNPI and SLC17A6, has a more restricted expression than the related VGLUT 1. Like VGLUT 1, it is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.