Home|||||||Technical Support|

VGLUT 2 - 135 421C5

Glutamate transporter in the membrane of synaptic vesicles
Monoclonal mouse purified IgG
Cat. No.: 135 421C5
Amount: 100 µg
Price: 465.00 €
Cat. No. 135 421C5 100 µg purified IgG, lyophilized, fluorescence-labeled with Sulfo-Cyanine 5. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications WB: N/A
IP: N/A
ICC: not recommended (see remarks)
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P/FFPE: not tested yet
Label Sulfo-Cyanine 5
Clone 95E11
Subtype IgG2a (κ light chain)
Immunogen Synthetic peptide corresponding to AA 566 to 582 from rat VGLUT2 (UniProt Id: Q9JI12)
Reactivity Reacts with: rat (Q9JI12), mouse (Q8BLE7).
Other species not tested yet.
Specificity Specific for VGLUT 2
Remarks

ICC: As a Sulfo-Cyanine 5 conjugate this antibody produces some unspecifc nuclear staining in cultured primary neurons.

Data sheet 135_421c5.pdf
Cat. No.: 135 421C5
Quantity: 100 µg
Price: 465.00 €
Background

The vesicular glutamate transporter 2 VGLUT 2, also referred to as DNPI and SLC17A6, has a more restricted expression than the related VGLUT 1. Like VGLUT 1, it is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.