|Cat. No. 135 421BT||100 µg purified IgG, lyophilized, biotin-labeled. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 up to 1 : 5000 (see remarks) gallery
IP: not tested yet
IHC: 1 : 500 up to 1 : 1000 gallery
IHC-P/FFPE: 1 : 100 gallery
|Subtype||IgG2a (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 566 to 582 from rat VGLUT2 (UniProt Id: Q9JI12)|
Reacts with: mouse (Q8BLE7), rat (Q9JI12).
Other species not tested yet.
|Specificity||Specific for VGLUT 2|
WB: This antibody is highly recommended for Western blot experiments. VGLUT 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
The vesicular glutamate transporter 2 VGLUT 2, also referred to as DNPI and SLC17A6, has a more restricted expression than the related VGLUT 1. Like VGLUT 1, it is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.