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VGLUT2 antibody - 135 409 K.O.

VGLUT2 is a glutamate transporter in the membrane of synaptic vesicles
Chicken monoclonal recombinant IgY
Cat. No.: 135 409
Amount: 50 µg
Price: $415.00
Cat. No. 135 409 50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP-staining) (see remarks) gallery  
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 500 up to 1 : 1000 gallery  
Clone Ch95E11
Subtype IgY (λ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT2 (UniProt Id: Q9JI12)
Reactivity Reacts with: rat (Q9JI12), mouse (Q8BLE7).
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 135-4P
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 95E11. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.
WB: VGLUT2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 135_409.pdf
Cat. No.: 135 409
Amount: 50 µg
Price: $415.00
Background

The vesicular glutamate transporter 2 VGLUT 2, also referred to as DNPI and SLC17A6, has a more restricted expression than the related VGLUT 1. Like VGLUT 1, it is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.