|Cat. No. 135 408||50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 up to 1 : 5000 (AP staining) (see remarks) gallery
ICC: 1 : 500 up to 1 : 1000 gallery
IHC: 1 : 200 up to 1 : 500 gallery
IHC-P/FFPE: 1 : 100 up to 1 : 1000 gallery
|Subtype||IgG1 (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 566 to 582 from rat VGLUT2 (UniProt Id: Q9JI12)|
Epitop: AA 566 to 582 from rat VGLUT2 (UniProt Id: Q9JI12)
Reacts with: rat (Q9JI12), mouse (Q8BLE7).
Other species not tested yet.
|Specificity||Specific for VGLUT 2.|
This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 95E11. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
WB: VGLUT 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
The vesicular glutamate transporter 2 VGLUT 2, also referred to as DNPI and SLC17A6, has a more restricted expression than the related VGLUT 1. Like VGLUT 1, it is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.