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VGLUT 2 antibody - 135 408

VGluT2 is a glutamate transporter in the membrane of synaptic vesicles
Rabbit monoclonal recombinant IgG
Cat. No.: 135 408
Amount: 50 µg
Price: $415.00
Cat. No. 135 408 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining​) (see remarks) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 100 up to 1 : 1000 gallery  
Clone Rb95E11
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT2 (UniProt Id: Q9JI12)
Epitop Epitop: AA 566 to 582 from rat VGLUT2 (UniProt Id: Q9JI12)
Reactivity Reacts with: rat (Q9JI12), mouse (Q8BLE7).
Other species not tested yet.
Specificity Specific for VGLUT 2. K.O. PubMed: 35584670
Matching control protein/peptide 135-4P
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 95E11. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
The antibody is highly recommended as a marker for glutamatergic nerve terminals.
WB: VGLUT 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 135_408.pdf

References for VGLUT 2 - 135 408

Glutamate released by Cajal-Retzius cells impacts specific hippocampal circuits and behaviors.
Anstötz M, Lee SK, Maccaferri G
Cell reports (2022) 397: 110822. 135 408 IHC; KO verified; tested species: mouse
Dorsal and ventral striatal neuronal subpopulations differentially disrupt male mouse copulatory behavior.
Detraux B, Vilella A, De Groote A, Schiffmann SN, Zoli M, de Kerchove d'Exaerde A
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology (2021) 49: 23-37. 135 408 IHC; tested species: mouse
Spinoparabrachial projection neurons form distinct classes in the mouse dorsal horn.
Browne TJ, Smith KM, Gradwell MA, Iredale JA, Dayas CV, Callister RJ, Hughes DI, Graham BA
Pain (2021) 1627: 1977-1994. 135 408 IHC; tested species: mouse
Cat. No.: 135 408
Amount: 50 µg
Price: $415.00
Glutamate released by Cajal-Retzius cells impacts specific hippocampal circuits and behaviors.
Anstötz M, Lee SK, Maccaferri G
Cell reports (2022) 397: 110822. 135 408 IHC; KO verified; tested species: mouse
Dorsal and ventral striatal neuronal subpopulations differentially disrupt male mouse copulatory behavior.
Detraux B, Vilella A, De Groote A, Schiffmann SN, Zoli M, de Kerchove d'Exaerde A
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology (2021) 49: 23-37. 135 408 IHC; tested species: mouse
Spinoparabrachial projection neurons form distinct classes in the mouse dorsal horn.
Browne TJ, Smith KM, Gradwell MA, Iredale JA, Dayas CV, Callister RJ, Hughes DI, Graham BA
Pain (2021) 1627: 1977-1994. 135 408 IHC; tested species: mouse
Background

The vesicular glutamate transporter 2 VGLUT 2, also referred to as DNPI and SLC17A6, has a more restricted expression than the related VGLUT 1. Like VGLUT 1, it is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.