Tailor-made Antibodies
and Tools for Life Science
- will be discontinued soon
Polyclonal antibodies are a finite resource. We have now started to sell the last lots of our well received Guinea pig polyclonal VGLUT2 antibody 135 404. It will be replaced by our new Guinea pig monoclonal recombinant IgG VGLUT2 antibody 135 418.

VGLUT2 antibody - 135 404 K.O.

VGluT2 is a glutamate transporter in the membrane of synaptic vesicles
Guinea pig polyclonal antiserum
Cat. No.: 135 404
Amount: 100 µl
Price: $405.00
Cat. No. 135 404 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 200 up to 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
iDISCO: yes
EM: yes
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT2 (UniProt Id: Q9JI12)
Reactivity Reacts with: rat (Q9JI12), mouse (Q8BLE7).
Other species not tested yet.
Specificity K.O. validated PubMed: 25357191
Matching control protein/peptide 135-4P
Remarks

This antibody is highly recommended as a marker for glutamatergic nerve terminals.
WB: VGLUT 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 135_404.pdf

References for VGLUT2 - 135 404

Vesicular Glutamate Transporters (SLCA17 A6, 7, 8) Control Synaptic Phosphate Levels.
Cheret C, Ganzella M, Preobraschenski J, Jahn R, Ahnert-Hilger G
Cell reports (2021) 342: 108623. 135 404 WB, ICC; tested species: human,mouse
Hevin-calcyon interaction promotes synaptic reorganization after brain injury.
Kim JH, Jung HG, Kim A, Shim HS, Hyeon SJ, Lee YS, Han J, Jung JH, Lee J, Ryu H, Park JY, et al.
Cell death and differentiation (2021) 289: 2571-2588. 135 404 WB, ICC, IHC; tested species: mouse
Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration.
Weinert S, Gimber N, Deuschel D, Stuhlmann T, Puchkov D, Farsi Z, Ludwig CF, Novarino G, López-Cayuqueo KI, Planells-Cases R, Jentsch TJ, et al.
The EMBO journal (2020) : e103358. 135 404 WB; tested species: mouse
VGLUT1 Binding to Endophilin or Intersectin1 and Dynamin Phosphorylation in a Diurnal Context.
Richter K, Schmutz I, Darna M, Zander JF, Chavan R, Albrecht U, Ahnert-Hilger G
Neuroscience (2018) 371: 29-37. 135 404 WB; tested species: mouse
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 135 404 WB
An essential role of acetylcholine-glutamate synergy at habenular synapses in nicotine dependence.
Frahm S, Antolin-Fontes B, Görlich A, Zander JF, Ahnert-Hilger G, Ibañez-Tallon I
eLife (2015) 4: e11396. 135 404 WB, IHC
Cat. No.: 135 404
Amount: 100 µl
Price: $405.00