Tailor-made Antibodies
and Tools for Life Science

VGLUT1 antibody - 135 318 K.O.

VGluT1 is a glutamate transporter in the membrane of synaptic vesicles
Guinea pig monoclonal recombinant IgG
Cat. No.: 135 318
Amount: 50 µg
Price: $420.00
Cat. No. 135 318 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP-staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 4000 gallery  
Clone Gp68B7
Subtype IgG2 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT 1. (UniProt Id: Q62634)
Reactivity Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 135-0P
Remarks

This antibody is a chimeric antibody based on the well-known monoclonal mouse antibody clone 68B7. The constant regions of the heavy and light chains have been replaced by Guinea pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
WB: This antibody is highly recommended for Western blot experiments. VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 135_318.pdf
Cat. No.: 135 318
Amount: 50 µg
Price: $420.00