Tailor-made Antibodies
and Tools for Life Science

VGLUT1 antibody - 135 303C5 K.O.

VGluT1 is a glutamate transporter in the membrane of synaptic vesicles
Rabbit polyclonal purified antibody
Cat. No.: 135 303C5
Amount: 50 µg
Price: $500.00
Cat. No. 135 303C5 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Cyanine 5.
Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 500 up to 1 : 10000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: not tested yet
Label Sulfo-Cyanine 5
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat VGLUT 1 (UniProt Id: Q62634)
Reactivity Reacts with: human (Q9P2U7), rat (Q62634), mouse (Q3TXX4), cow, goat, sheep, dog.
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 135-3P
Remarks

This antibody is highly recommended as markers for glutamatergic nerve terminals.

Data sheet 135_303c5.pdf

References for VGLUT1 - 135 303C5

Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration.
Weinert S, Gimber N, Deuschel D, Stuhlmann T, Puchkov D, Farsi Z, Ludwig CF, Novarino G, López-Cayuqueo KI, Planells-Cases R, Jentsch TJ, et al.
The EMBO journal (2020) : e103358. 135 303C5 ICC; tested species: mouse
Modulation of P2X7 purinergic receptor activity by extracellular Zn2+ in cultured mouse hippocampal astroglia.
Kovács G, Környei Z, Tóth K, Baranyi M, Brunner J, Neubrandt M, Dénes Á, Sperlágh B
Cell calcium (2018) 75: 1-13. 135 303C5 ICC; tested species: mouse
Gray matter NG2 cells display multiple Ca2+-signaling pathways and highly motile processes.
Haberlandt C, Derouiche A, Wyczynski A, Haseleu J, Pohle J, Karram K, Trotter J, Seifert G, Frotscher M, Steinhäuser C, Jabs R, et al.
PloS one (2011) 63: e17575. 135 303C5 ICC; tested species: mouse
Cat. No.: 135 303C5
Amount: 50 µg
Price: $500.00