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VGLUT 1 - 135 303C5

Glutamate transporter in the membrane of synaptic vesicles
Polyclonal rabbit purified antibody
Cat. No.: 135 303C5
Amount: 50 µg
Price: $475.00
Cat. No. 135 303C5 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Oyster® 650

Fluorescence labeled antibodies conjugated to Oyster dyes from Luminartis GmbH (formerly Denovo Biolabels) allow the usage of established CyDye fluorescence detection systems. Oyster-488 has similar exitation and emission properties as Cy2, Oyster-550 as Cy3, and Oyster-650 is comparable to Cy5.
For our custom antibody labeling service Oyster-500 is also available.
Oyster dyes do not form dimers and therefore exhibit less internal quenching. This allows a higher labeling degree and consequently brighter conjugates.

Oyster dyes, Oyster-488, Oyster-500, Oyster-550, and Oyster-650 are trademarks of Luminartis GmbH, Muenster/Germany.

CyDyes, Cy2, Cy3, and Cy5 are trademarks of GE-Healthcare Ltd.

. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.The mounting agent Aquatex®(Merck Chemicals) is not compatible with Oyster dyes!
Applications WB: N/A
IP: N/A
ICC: 1 : 500 up to 1 : 10000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P/FFPE: not tested yet
Label Oyster 650
Immunogen Recombinant protein corresponding to AA 456 to 560 from rat VGLUT1 (UniProt Id: Q62634)
Reactivity Reacts with: human (Q9P2U7), rat (Q62634), mouse (Q3TXX4), cow, goat, sheep, dog.
Other species not tested yet.
Specificity Specific for VGLUT 1. K.O.
Matching control protein/peptide 135-3P
Remarks

This antibody is highly recommended as markers for glutamatergic nerve terminals.

Data sheet 135_303c5.pdf

References for VGLUT 1 - 135 303C5

Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration.
Weinert S, Gimber N, Deuschel D, Stuhlmann T, Puchkov D, Farsi Z, Ludwig CF, Novarino G, López-Cayuqueo KI, Planells-Cases R, Jentsch TJ, et al.
The EMBO journal (2020) : e103358. 135 303C5 ICC; tested species: mouse
Modulation of P2X7 purinergic receptor activity by extracellular Zn2+ in cultured mouse hippocampal astroglia.
Kovács G, Környei Z, Tóth K, Baranyi M, Brunner J, Neubrandt M, Dénes Á, Sperlágh B
Cell calcium (2018) 75: 1-13. 135 303C5 ICC; tested species: mouse
Gray matter NG2 cells display multiple Ca2+-signaling pathways and highly motile processes.
Haberlandt C, Derouiche A, Wyczynski A, Haseleu J, Pohle J, Karram K, Trotter J, Seifert G, Frotscher M, Steinhäuser C, Jabs R, et al.
PloS one (2011) 63: e17575. 135 303C5 ICC; tested species: mouse
Microglial Displacement of GABAergic Synapses Is a Protective Event during Complex Febrile Seizures.
Wan Y, Feng B, You Y, Yu J, Xu C, Dai H, Trapp BD, Shi P, Chen Z, Hu W
Cell reports (2020) 335: 108346. 135 303C5 IHC; tested species: mouse
Cat. No.: 135 303C5
Quantity: 50 µg
Price: $475.00
Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration.
Weinert S, Gimber N, Deuschel D, Stuhlmann T, Puchkov D, Farsi Z, Ludwig CF, Novarino G, López-Cayuqueo KI, Planells-Cases R, Jentsch TJ, et al.
The EMBO journal (2020) : e103358. 135 303C5 ICC; tested species: mouse
Modulation of P2X7 purinergic receptor activity by extracellular Zn2+ in cultured mouse hippocampal astroglia.
Kovács G, Környei Z, Tóth K, Baranyi M, Brunner J, Neubrandt M, Dénes Á, Sperlágh B
Cell calcium (2018) 75: 1-13. 135 303C5 ICC; tested species: mouse
Gray matter NG2 cells display multiple Ca2+-signaling pathways and highly motile processes.
Haberlandt C, Derouiche A, Wyczynski A, Haseleu J, Pohle J, Karram K, Trotter J, Seifert G, Frotscher M, Steinhäuser C, Jabs R, et al.
PloS one (2011) 63: e17575. 135 303C5 ICC; tested species: mouse
Microglial Displacement of GABAergic Synapses Is a Protective Event during Complex Febrile Seizures.
Wan Y, Feng B, You Y, Yu J, Xu C, Dai H, Trapp BD, Shi P, Chen Z, Hu W
Cell reports (2020) 335: 108346. 135 303C5 IHC; tested species: mouse
Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.