Home|||||||Technical Support|

VGLUT 1 - 135 011BT

Glutamate transporter in the membrane of synaptic vesicles
Monoclonal mouse purified IgG
Cat. No.: 135 011BT
Amount: 100 µg
Price: 465.00 €
Cat. No. 135 011BT 100 µg purified IgG, lyophilized, biotin-labeled. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 (see remarks) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 200 up to 1 : 1000 gallery  
Label biotin
Clone 68B7
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 542 to 560 from rat VGLUT1 (UniProt Id: Q62634)
Reactivity Reacts with: mouse (Q3TXX4), rat (Q62634).
Other species not tested yet.
Specificity Specific for VGLUT 1 K.O.
Matching control protein/peptide 135-0P
Remarks

WB: This antibody is highly recommended for Western blot experiments. VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples

Data sheet 135_011bt.pdf
Cat. No.: 135 011BT
Quantity: 100 µg
Price: 465.00 €
Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.