Cat. No. 132 003CpH |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with
CypHer5E.
Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
Applications | |
Label | CypHer5E |
Immunogen | Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212) |
Specificity | Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives. |
Remarks |
This antibody is suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment. |
Data sheet | 132_003cph.pdf |