Tailor-made Antibodies
and Tools for Life Science

VGAT antibody luminal domain - 131 103C3 K.O.

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Rabbit polyclonal purified antibody
Cat. No.: 131 103C3
Amount: 50 µg
Price: $785.00
Cat. No. 131 103C3 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Cyanine 3.
Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 200 (see remarks) gallery  
IHC: yes
IHC-P: not tested yet
Label Sulfo-Cyanine 3
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633).
Other species not tested yet.
Specificity K.O. validated
Remarks

ICC: This antibody is intended for labeling of recycling synaptic vesicles at inhibitory nerve terminals by adding to living neurons. Further details see Martens et al. 2008.

Data sheet 131_103c3.pdf

References for VGAT - 131 103C3

Clathrin-independent endocytic retrieval of SV proteins mediated by the clathrin adaptor AP-2 at mammalian central synapses.
López-Hernández T, Takenaka KI, Mori Y, Kongpracha P, Nagamori S, Haucke V, Takamori S
eLife (2022) 11: . 131 103C3 ICC; tested species: mouse
Endogenous β-neurexins on axons and within synapses show regulated dynamic behavior.
Klatt O, Repetto D, Brockhaus J, Reissner C, El Khallouqi A, Rohlmann A, Heine M, Missler M
Cell reports (2021) 3511: 109266. 131 103C3 ICC, UPTAKE; tested species: mouse
Inter-Synaptic Lateral Diffusion of GABAA Receptors Shapes Inhibitory Synaptic Currents.
de Luca E, Ravasenga T, Petrini EM, Polenghi A, Nieus T, Guazzi S, Barberis A
Neuron (2017) 951: 63-69.e5. 131 103C3 ICC; tested species: mouse
Regulated Dynamic Trafficking of Neurexins Inside and Outside of Synaptic Terminals.
Neupert C, Schneider R, Klatt O, Reissner C, Repetto D, Biermann B, Niesmann K, Missler M, Heine M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3540: 13629-47. 131 103C3 ICC
Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP.
Petrini EM, Ravasenga T, Hausrat TJ, Iurilli G, Olcese U, Racine V, Sibarita JB, Jacob TC, Moss SJ, Benfenati F, Medini P, et al.
Nature communications (2014) 5: 3921. 131 103C3 ICC, IHC; tested species: mouse
Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
Martens H, Weston MC, Boulland JL, Grønborg M, Grosche J, Kacza J, Hoffmann A, Matteoli M, Takamori S, Harkany T, Chaudhry FA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2849: 13125-31. 131 103C3 ICC, UPTAKE; tested species: rat
Cat. No.: 131 103C3
Amount: 50 µg
Price: $785.00