Home|||||||Technical Support|

VGAT lumenal domain - 131 103C3

GABA transporter in the membrane of synaptic vesicles
Polyclonal rabbit purified antibody
Cat. No.: 131 103C3
Amount: 50 µg
Price: 775.00 €
Cat. No. 131 103C3 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Sulfo-Cyanine 3. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 200 gallery  
IHC: yes
IHC-P/FFPE: not tested yet
Label Sulfo-Cyanine 3
Immunogen Synthetic peptide corresponding to AA 510 to 525 from rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633).
Other species not tested yet.
Specificity Specific for VGAT. K.O.
Remarks This antibody is intended for labeling of recycling synaptic vesicles at inhibitory nerve terminals by adding to living neurons. Further details see Martens et al. 2008.
Data sheet 131_103c3.pdf

References for VGAT - 131 103C3

Inter-Synaptic Lateral Diffusion of GABAA Receptors Shapes Inhibitory Synaptic Currents.
de Luca E, Ravasenga T, Petrini EM, Polenghi A, Nieus T, Guazzi S, Barberis A
Neuron (2017) 951: 63-69.e5. 131 103C3 ICC; tested species: mouse
Regulated Dynamic Trafficking of Neurexins Inside and Outside of Synaptic Terminals.
Neupert C, Schneider R, Klatt O, Reissner C, Repetto D, Biermann B, Niesmann K, Missler M, Heine M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3540: 13629-47. 131 103C3 ICC
Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP.
Petrini EM, Ravasenga T, Hausrat TJ, Iurilli G, Olcese U, Racine V, Sibarita JB, Jacob TC, Moss SJ, Benfenati F, Medini P, et al.
Nature communications (2014) 5: 3921. 131 103C3 ICC, IHC; tested species: mouse
Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
Martens H, Weston MC, Boulland JL, Grønborg M, Grosche J, Kacza J, Hoffmann A, Matteoli M, Takamori S, Harkany T, Chaudhry FA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2849: 13125-31. 131 103C3 ICC, UPTAKE; tested species: rat
Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP.
Petrini EM, Ravasenga T, Hausrat TJ, Iurilli G, Olcese U, Racine V, Sibarita JB, Jacob TC, Moss SJ, Benfenati F, Medini P, et al.
Nature communications (2014) 5: 3921. 131 103C3 ICC, IHC; tested species: mouse
Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling.
Fricke S, Metzdorf K, Ohm M, Haak S, Heine M, Korte M, Zagrebelsky M
Cell reports (2019) 293: 671-684.e6. 131 103C3 UPTAKE; tested species: mouse
Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling.
Pan PY, Marrs J, Ryan TA
The Journal of biological chemistry (2015) 29037: 22593-601. 131 103C3 UPTAKE
Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
Martens H, Weston MC, Boulland JL, Grønborg M, Grosche J, Kacza J, Hoffmann A, Matteoli M, Takamori S, Harkany T, Chaudhry FA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2849: 13125-31. 131 103C3 ICC, UPTAKE; tested species: rat
Activity-Dependent Inhibitory Synapse Scaling Is Determined by Gephyrin Phosphorylation and Subsequent Regulation of GABAA Receptor Diffusion.
Battaglia S, Renner M, Russeau M, Côme E, Tyagarajan SK, Lévi S
eNeuro () 51: . 131 103C3 UPTAKE; tested species: rat
Cat. No.: 131 103C3
Quantity: 50 µg
Price: 775.00 €
Inter-Synaptic Lateral Diffusion of GABAA Receptors Shapes Inhibitory Synaptic Currents.
de Luca E, Ravasenga T, Petrini EM, Polenghi A, Nieus T, Guazzi S, Barberis A
Neuron (2017) 951: 63-69.e5. 131 103C3 ICC; tested species: mouse
Regulated Dynamic Trafficking of Neurexins Inside and Outside of Synaptic Terminals.
Neupert C, Schneider R, Klatt O, Reissner C, Repetto D, Biermann B, Niesmann K, Missler M, Heine M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3540: 13629-47. 131 103C3 ICC
Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP.
Petrini EM, Ravasenga T, Hausrat TJ, Iurilli G, Olcese U, Racine V, Sibarita JB, Jacob TC, Moss SJ, Benfenati F, Medini P, et al.
Nature communications (2014) 5: 3921. 131 103C3 ICC, IHC; tested species: mouse
Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
Martens H, Weston MC, Boulland JL, Grønborg M, Grosche J, Kacza J, Hoffmann A, Matteoli M, Takamori S, Harkany T, Chaudhry FA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2849: 13125-31. 131 103C3 ICC, UPTAKE; tested species: rat
Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP.
Petrini EM, Ravasenga T, Hausrat TJ, Iurilli G, Olcese U, Racine V, Sibarita JB, Jacob TC, Moss SJ, Benfenati F, Medini P, et al.
Nature communications (2014) 5: 3921. 131 103C3 ICC, IHC; tested species: mouse
Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling.
Fricke S, Metzdorf K, Ohm M, Haak S, Heine M, Korte M, Zagrebelsky M
Cell reports (2019) 293: 671-684.e6. 131 103C3 UPTAKE; tested species: mouse
Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling.
Pan PY, Marrs J, Ryan TA
The Journal of biological chemistry (2015) 29037: 22593-601. 131 103C3 UPTAKE
Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
Martens H, Weston MC, Boulland JL, Grønborg M, Grosche J, Kacza J, Hoffmann A, Matteoli M, Takamori S, Harkany T, Chaudhry FA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2849: 13125-31. 131 103C3 ICC, UPTAKE; tested species: rat
Activity-Dependent Inhibitory Synapse Scaling Is Determined by Gephyrin Phosphorylation and Subsequent Regulation of GABAA Receptor Diffusion.
Battaglia S, Renner M, Russeau M, Côme E, Tyagarajan SK, Lévi S
eNeuro () 51: . 131 103C3 UPTAKE; tested species: rat
Background
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.