Tailor-made Antibodies
and Tools for Life Science

VGAT antibody luminal domain - 131 103AF

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles

pH-sensitive probe fluorescent only under acidic conditions

Rabbit polyclonal purified antibody
Cat. No.: 131 103AF
Amount: 50 µg
Price: $785.00
Cat. No. 131 103AF 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with AcidiFluor Orange.
Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 300 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
Label AcidiFluor Orange
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: mouse (O35633), rat (O35458).
Other species not tested yet.
Remarks

ICC: This antibody can only be used for labeling of recycling synaptic vesicles in living neurons. It is not recommended for the staining of fixed cells.
The pH sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen.

Data sheet 131_103af.pdf
Cat. No.: 131 103AF
Amount: 50 µg
Price: $785.00