Cat. No.: 131 103AF
Amount: 50 µg
Price:
$785.00
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pH-sensitive probe fluorescent only under acidic conditions
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| Cat. No. 131 103AF |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with AcidiFluor Orange. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications |
IP: N/A ICC: 1 : 100 up to 1 : 300 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet |
| Label | AcidiFluor Orange |
| Immunogen | Synthetic peptide corresponding to residues near the carboxy terminus of rat VGAT (UniProt Id: O35458) |
| Reactivity |
Reacts with: mouse (O35633), rat (O35458). Other species not tested yet. |
| Remarks |
ICC: This antibody is intended for direct labeling of recycling synaptic vesicles at inhibitory nerve terminals in living primary neuronal cultures. The pH sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen. Further details see Martens et al. 2008. |
| Data sheet | 131_103af.pdf |
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