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VGAT lumenal domain - 131 103AF

GABA transporter in the membrane of synaptic vesicles
Polyclonal rabbit purified antibody
Cat. No.: 131 103AF
Amount: 50 µg
Price: 775.00 €
Cat. No. 131 103AF 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with AcidiFluor Orange 

AcidiFluor Orange® is a pH-sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the cellular compartment e.g. the synaptic vesicle.
AcidiFluor Orange can be detected with the filter sets for Cy3.

CyDyes, Cy2, Cy3, and Cy5 are trademarks of GE-Healthcare Ltd.

This product or portions thereof is manufactured under license from Tokyo University and is covered by Patents Application Number US 2010068733, US 20140248654, EP2098529A1/B1, JP WO 2013035767A, JP 5636080B2, WO 2008/0599910A1, WO 2016/1579937A1 and issued patents US 8258171 and US 9784732 and related patents. Acidifluor®, and Acidifluor Orange® and related products are trademarks of Goryo Chemical. Purchase of reagents related to the Acidifluor Series technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from: Goryo Chemical, EAREE Bldg., SF Kita 8 Nishi 18-35-100, Chuo-Ku, Sapporo, 060-0008 Japan.

. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 300 (see remarks) gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Label AcidiFluor Orange
Immunogen Synthetic peptide corresponding to AA 510 to 525 from rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: mouse (O35633), rat (O35458).
Other species not tested yet.
Specificity specific for VGAT
Remarks

ICC: This antibody is intended for direct labeling of recycling synaptic vesicles at inhibitory nerve terminals in living primary neuronal cultures. The pH sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen. For further details see Martens et al. 2008.

Data sheet 131_103af.pdf
Cat. No.: 131 103AF
Quantity: 50 µg
Price: 775.00 €
Background
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.
Protocols