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Neuroligin 2 antibody - 129 511

Neuroligins are postsynaptic cell adhesion molecules
Mouse monoclonal purified IgG
Cat. No.: 129 511
Amount: 100 µg
Price: $415.00
Cat. No. 129 511 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 200 gallery  
Clone 5E6
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 750 to 767 from rat Neuroligin2 (UniProt Id: Q62888)
Epitop Epitop: AA 750 to 767 from rat Neuroligin2 (UniProt Id: Q62888)
Reactivity Reacts with: human (Q8NFZ4), rat (Q62888), mouse (Q69ZK9).
Other species not tested yet.
Specificity Specific for neuroligin 2 . K.O. PubMed: 29107521
Remarks

IHC: Fix for 15 min with 4% PFA and 15% picric acid in PBS.

Data sheet 129_511.pdf

References for Neuroligin 2 - 129 511

Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning.
Soleimanpour E, Bergado Acosta JR, Landgraf P, Mayer D, Dankert E, Dieterich DC, Fendt M
Cells (2021) 102: . 129 511 WB; tested species: rat
SPARCL1 Promotes Excitatory But Not Inhibitory Synapse Formation and Function Independent of Neurexins and Neuroligins.
Gan KJ, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4042: 8088-8102. 129 511 WB; KO verified; tested species: mouse
Synaptic Kalirin-7 and Trio Interactomes Reveal a GEF Protein-Dependent Neuroligin-1 Mechanism of Action.
Paskus JD, Tian C, Fingleton E, Shen C, Chen X, Li Y, Myers SA, Badger JD, Bemben MA, Herring BE, Roche KW, et al.
Cell reports (2019) 2910: 2944-2952.e5. 129 511 WB; tested species: rat
Sorting nexin 27 rescues neuroligin 2 from lysosomal degradation to control inhibitory synapse number.
Binda C, Nakamura Y, Henley J, Wilkinson K
The Biochemical journal (2019) : . 129 511 WB, ICC; tested species: rat
Neuroligin 1, 2, and 3 Regulation at the Synapse: FMRP-Dependent Translation and Activity-Induced Proteolytic Cleavage.
Chmielewska JJ, Kuzniewska B, Milek J, Urbanska K, Dziembowska M
Molecular neurobiology (2018) : . 129 511 WB; tested species: mouse
Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.
Li J, Han W, Pelkey KA, Duan J, Mao X, Wang YX, Craig MT, Dong L, Petralia RS, McBain CJ, Lu W, et al.
Neuron (2017) 964: 808-826.e8. 129 511 WB, ICC, IHC; KO verified; tested species: mouse
Unique versus Redundant Functions of Neuroligin Genes in Shaping Excitatory and Inhibitory Synapse Properties.
Chanda S, Hale WD, Zhang B, Wernig M, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3729: 6816-6836. 129 511 WB; tested species: mouse
IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2α.
Tanabe Y, Naito Y, Vasuta C, Lee AK, Soumounou Y, Linhoff MW, Takahashi H
Nature communications (2017) 81: 408. 129 511 WB
A novel synaptic junction preparation for the identification and characterization of cleft proteins.
Burch A, Tao-Cheng JH, Dosemeci A
PloS one (2017) 123: e0174895. 129 511 WB; tested species: rat
Neurotrophin-3 Regulates Synapse Development by Modulating TrkC-PTPσ Synaptic Adhesion and Intracellular Signaling Pathways.
Han KA, Woo D, Kim S, Choii G, Jeon S, Won SY, Kim HM, Heo WD, Um JW, Ko J
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3617: 4816-31. 129 511 WB
Stepwise disassembly of GABAergic synapses during pathogenic excitotoxicity.
Garcia JD, Gookin SE, Crosby KC, Schwartz SL, Tiemeier E, Kennedy MJ, Dell'Acqua ML, Herson PS, Quillinan N, Smith KR
Cell reports (2021) 3712: 110142. 129 511 ICC; tested species: rat
In trans neuregulin3-Caspr3 interaction controls DA axonal bassoon cluster development.
Cui W, Gao N, Dong Z, Shen C, Zhang H, Luo B, Chen P, Comoletti D, Jing H, Wang H, Robinson H, et al.
Current biology : CB (2021) 3115: 3330-3342.e7. 129 511 ICC; tested species: mouse
Sorting nexin 27 rescues neuroligin 2 from lysosomal degradation to control inhibitory synapse number.
Binda C, Nakamura Y, Henley J, Wilkinson K
The Biochemical journal (2019) : . 129 511 WB, ICC; tested species: rat
Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.
Li J, Han W, Pelkey KA, Duan J, Mao X, Wang YX, Craig MT, Dong L, Petralia RS, McBain CJ, Lu W, et al.
Neuron (2017) 964: 808-826.e8. 129 511 WB, ICC, IHC; KO verified; tested species: mouse
GSK3 and KIF5 regulate activity-dependent sorting of gephyrin between axons and dendrites.
Rathgeber L, Gromova KV, Schaefer I, Breiden P, Lohr C, Kneussel M
European journal of cell biology () 943-4: 173-8. 129 511 ICC
Neurexin-3 defines synapse- and sex-dependent diversity of GABAergic inhibition in ventral subiculum.
Boxer EE, Seng C, Lukacsovich D, Kim J, Schwartz S, Kennedy MJ, Földy C, Aoto J
Cell reports (2021) 3710: 110098. 129 511 IHC; tested species: mouse
IgSF9b regulates anxiety behaviors through effects on centromedial amygdala inhibitory synapses.
Babaev O, Cruces-Solis H, Piletti Chatain C, Hammer M, Wenger S, Ali H, Karalis N, de Hoz L, Schlüter OM, Yanagawa Y, Ehrenreich H, et al.
Nature communications (2018) 91: 5400. 129 511 IHC; tested species: mouse
Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.
Li J, Han W, Pelkey KA, Duan J, Mao X, Wang YX, Craig MT, Dong L, Petralia RS, McBain CJ, Lu W, et al.
Neuron (2017) 964: 808-826.e8. 129 511 WB, ICC, IHC; KO verified; tested species: mouse
Cat. No.: 129 511
Amount: 100 µg
Price: $415.00
Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning.
Soleimanpour E, Bergado Acosta JR, Landgraf P, Mayer D, Dankert E, Dieterich DC, Fendt M
Cells (2021) 102: . 129 511 WB; tested species: rat
SPARCL1 Promotes Excitatory But Not Inhibitory Synapse Formation and Function Independent of Neurexins and Neuroligins.
Gan KJ, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4042: 8088-8102. 129 511 WB; KO verified; tested species: mouse
Synaptic Kalirin-7 and Trio Interactomes Reveal a GEF Protein-Dependent Neuroligin-1 Mechanism of Action.
Paskus JD, Tian C, Fingleton E, Shen C, Chen X, Li Y, Myers SA, Badger JD, Bemben MA, Herring BE, Roche KW, et al.
Cell reports (2019) 2910: 2944-2952.e5. 129 511 WB; tested species: rat
Sorting nexin 27 rescues neuroligin 2 from lysosomal degradation to control inhibitory synapse number.
Binda C, Nakamura Y, Henley J, Wilkinson K
The Biochemical journal (2019) : . 129 511 WB, ICC; tested species: rat
Neuroligin 1, 2, and 3 Regulation at the Synapse: FMRP-Dependent Translation and Activity-Induced Proteolytic Cleavage.
Chmielewska JJ, Kuzniewska B, Milek J, Urbanska K, Dziembowska M
Molecular neurobiology (2018) : . 129 511 WB; tested species: mouse
Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.
Li J, Han W, Pelkey KA, Duan J, Mao X, Wang YX, Craig MT, Dong L, Petralia RS, McBain CJ, Lu W, et al.
Neuron (2017) 964: 808-826.e8. 129 511 WB, ICC, IHC; KO verified; tested species: mouse
Unique versus Redundant Functions of Neuroligin Genes in Shaping Excitatory and Inhibitory Synapse Properties.
Chanda S, Hale WD, Zhang B, Wernig M, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3729: 6816-6836. 129 511 WB; tested species: mouse
IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2α.
Tanabe Y, Naito Y, Vasuta C, Lee AK, Soumounou Y, Linhoff MW, Takahashi H
Nature communications (2017) 81: 408. 129 511 WB
A novel synaptic junction preparation for the identification and characterization of cleft proteins.
Burch A, Tao-Cheng JH, Dosemeci A
PloS one (2017) 123: e0174895. 129 511 WB; tested species: rat
Neurotrophin-3 Regulates Synapse Development by Modulating TrkC-PTPσ Synaptic Adhesion and Intracellular Signaling Pathways.
Han KA, Woo D, Kim S, Choii G, Jeon S, Won SY, Kim HM, Heo WD, Um JW, Ko J
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3617: 4816-31. 129 511 WB
Stepwise disassembly of GABAergic synapses during pathogenic excitotoxicity.
Garcia JD, Gookin SE, Crosby KC, Schwartz SL, Tiemeier E, Kennedy MJ, Dell'Acqua ML, Herson PS, Quillinan N, Smith KR
Cell reports (2021) 3712: 110142. 129 511 ICC; tested species: rat
In trans neuregulin3-Caspr3 interaction controls DA axonal bassoon cluster development.
Cui W, Gao N, Dong Z, Shen C, Zhang H, Luo B, Chen P, Comoletti D, Jing H, Wang H, Robinson H, et al.
Current biology : CB (2021) 3115: 3330-3342.e7. 129 511 ICC; tested species: mouse
Sorting nexin 27 rescues neuroligin 2 from lysosomal degradation to control inhibitory synapse number.
Binda C, Nakamura Y, Henley J, Wilkinson K
The Biochemical journal (2019) : . 129 511 WB, ICC; tested species: rat
Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.
Li J, Han W, Pelkey KA, Duan J, Mao X, Wang YX, Craig MT, Dong L, Petralia RS, McBain CJ, Lu W, et al.
Neuron (2017) 964: 808-826.e8. 129 511 WB, ICC, IHC; KO verified; tested species: mouse
GSK3 and KIF5 regulate activity-dependent sorting of gephyrin between axons and dendrites.
Rathgeber L, Gromova KV, Schaefer I, Breiden P, Lohr C, Kneussel M
European journal of cell biology () 943-4: 173-8. 129 511 ICC
Neurexin-3 defines synapse- and sex-dependent diversity of GABAergic inhibition in ventral subiculum.
Boxer EE, Seng C, Lukacsovich D, Kim J, Schwartz S, Kennedy MJ, Földy C, Aoto J
Cell reports (2021) 3710: 110098. 129 511 IHC; tested species: mouse
IgSF9b regulates anxiety behaviors through effects on centromedial amygdala inhibitory synapses.
Babaev O, Cruces-Solis H, Piletti Chatain C, Hammer M, Wenger S, Ali H, Karalis N, de Hoz L, Schlüter OM, Yanagawa Y, Ehrenreich H, et al.
Nature communications (2018) 91: 5400. 129 511 IHC; tested species: mouse
Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.
Li J, Han W, Pelkey KA, Duan J, Mao X, Wang YX, Craig MT, Dong L, Petralia RS, McBain CJ, Lu W, et al.
Neuron (2017) 964: 808-826.e8. 129 511 WB, ICC, IHC; KO verified; tested species: mouse
Background

Neuroligins form a family of postsynaptic cell surface molecules that interact with β-neurexins. They are 110-120 kDa polypeptides with homology to acetylcholine esterase. Neuroligin 1 and neuroligin 3 are specifically localized to post-synaptic densities of excitatory synapses whereas neuroligin 2 is found exclusively on inhibitory synapses.
Mutations in neuroligin 3 and neuroligin 4 have been implicated with a rare, heritable form of autism.