|Cat. No. 129 508||50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (AP staining) gallery
IP: not tested yet
ICC: 1 : 500 gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Subtype||IgG1 (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 750 to 767 from rat Neuroligin2 (UniProt Id: Q62888)|
Epitop: AA 750 to 767 from rat Neuroligin2 (UniProt Id: Q62888)
Reacts with: mouse (Q69ZK9), rat (Q62888), human (Q8NFZ4).
Other species not tested yet.
|Specificity||Specific for neuroligin 2 K.O.|
This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 5E6. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Neuroligins form a family of postsynaptic cell surface molecules that interact with β-neurexins. They are 110-120 kDa polypeptides with homology to acetylcholine esterase. Neuroligin 1 and neuroligin 3 are specifically localized to post-synaptic densities of excitatory synapses whereas neuroligin 2 is found exclusively on inhibitory synapses.
Mutations in neuroligin 3 and neuroligin 4 have been implicated with a rare, heritable form of autism.