Tailor-made Antibodies
and Tools for Life Science
Home|||||Technical Support

Neuroligin2 antibody - 129 508 K.O.

Neuroligins are postsynaptic cell adhesion molecules
Rabbit monoclonal recombinant IgG
Cat. No.: 129 508
Amount: 50 µg
Price: $415.00
Cat. No. 129 508 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 200 up to 1 : 1000 gallery  
Clone Rb5E6
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 750 to 767 from rat Neuroligin2 (UniProt Id: Q62888)
Reactivity Reacts with: mouse (Q69ZK9), rat (Q62888), human (Q8NFZ4).
Other species not tested yet.
Specificity Synthetic peptide corresponding to residues surrounding AA760 of rat Neuroligin2 K.O. validated
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 5E6. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC: Mild fixation or heat-mediated antigen retrieval (in citrate buffer pH 6) can improve signal quality.

Data sheet 129_508.pdf
Cat. No.: 129 508
Amount: 50 µg
Price: $415.00
Background

Neuroligins form a family of postsynaptic cell surface molecules that interact with β-neurexins. They are 110-120 kDa polypeptides with homology to acetylcholine esterase. Neuroligin1 and neuroligin3 are specifically localized to post-synaptic densities of excitatory synapses whereas neuroligin2 is found exclusively on inhibitory synapses.
Mutations in neuroligin3 and neuroligin4 have been implicated with a rare, heritable form of autism.