Synaptic Systems - Neuroligin2

Cat. No.: 129 202

Amount: 200 µl

Price: $360.00

Cat. No. 129 202	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not recommended (see remarks) IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 732 to 749 and 750 to 767 from rat Neuroligin2 (UniProt Id: Q62888)
Reactivity	Reacts with: human (Q8NFZ4), rat (Q62888), mouse (Q69ZK9), monkey, ape, cow. Other species not tested yet.
Specificity	K.O. validated
Remarks	ICC: The affinity purified antibody (cat. no. 129 203) is highly recommended for ICC.
Data sheet	129_202.pdf

References for Neuroligin2 - 129 202

Phosphorylation of neuroligin-2 by PKA regulates its cell surface abundance and synaptic stabilization.
Halff EF, Hannan S, Kwanthongdee J, Lesept F, Smart TG, Kittler JT
Science signaling (2022) 15739: eabg2505. 129 202 WB; tested species: rat

PSD-95 deficiency alters GABAergic inhibition in the prefrontal cortex.
McEachern EP, Coley AA, Yang SS, Gao WJ
Neuropharmacology (2020) 179: 108277. 129 202 WB, IP; tested species: mouse

Chemico-genetic discovery of astrocytic control of inhibition in vivo.
Takano T, Wallace JT, Baldwin KT, Purkey AM, Uezu A, Courtland JL, Soderblom EJ, Shimogori T, Maness PF, Eroglu C, Soderling SH, et al.
Nature (2020) : . 129 202 WB; tested species: mouse

LAR-RPTPs Directly Interact with Neurexins to Coordinate Bidirectional Assembly of Molecular Machineries.
Han KA, Kim YJ, Yoon TH, Kim H, Bae S, Um JW, Choi SY, Ko J
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 129 202 WB; tested species: rat

Elevated protein synthesis in microglia causes autism-like synaptic and behavioral aberrations.
Xu ZX, Kim GH, Tan JW, Riso AE, Sun Y, Xu EY, Liao GY, Xu H, Lee SH, Do NY, Lee CH, et al.
Nature communications (2020) 111: 1797. 129 202 WB; tested species: mouse

A sex difference in the response of the rodent postsynaptic density to synGAP haploinsufficiency.
Mastro TL, Preza A, Basu S, Chattarji S, Till SM, Kind PC, Kennedy MB
eLife (2020) 9: . 129 202 WB; tested species: rat

SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling.
Halff EF, Szulc BR, Lesept F, Kittler JT
Cell reports (2019) 299: 2599-2607.e6. 129 202 WB; tested species: rat

Autism and Schizophrenia-Associated CYFIP1 Regulates the Balance of Synaptic Excitation and Inhibition.
Davenport EC, Szulc BR, Drew J, Taylor J, Morgan T, Higgs NF, López-Doménech G, Kittler JT
Cell reports (2019) 268: 2037-2051.e6. 129 202 WB; tested species: mouse

A unique intracellular tyrosine in neuroligin-1 regulates AMPA receptor recruitment during synapse differentiation and potentiation.
Letellier M, Szíber Z, Chamma I, Saphy C, Papasideri I, Tessier B, Sainlos M, Czöndör K, Thoumine O
Nature communications (2018) 91: 3979. 129 202 WB; tested species: rat

GABAergic deficits and schizophrenia-like behaviors in a mouse model carrying patient-derived neuroligin-2 R215H mutation.
Jiang DY, Wu Z, Forsyth CT, Hu Y, Yee SP, Chen G
Molecular brain (2018) 111: 31. 129 202 WB; tested species: mouse

Structural Insights into Modulation of Neurexin-Neuroligin Trans-synaptic Adhesion by MDGA1/Neuroligin-2 Complex.
Kim JA, Kim D, Won SY, Han KA, Park D, Cho E, Yun N, An HJ, Um JW, Kim E, Lee JO, et al.
Neuron (2017) 946: 1121-1131.e6. 129 202 WB; tested species: rat

Assembly rules for GABAA receptor complexes in the brain.
Martenson JS, Yamasaki T, Chaudhury NH, Albrecht D, Tomita S
eLife (2017) 6: . 129 202 WB; tested species: mouse

Cadherin-10 Maintains Excitatory/Inhibitory Ratio through Interactions with Synaptic Proteins.
Smith KR, Jones KA, Kopeikina KJ, Burette AC, Copits BA, Yoon S, Forrest MP, Fawcett-Patel JM, Hanley JG, Weinberg RJ, Swanson GT, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3746: 11127-11139. 129 202 WB; tested species: rat

GARLH Family Proteins Stabilize GABAA Receptors at Synapses.
Yamasaki T, Hoyos-Ramirez E, Martenson JS, Morimoto-Tomita M, Tomita S
Neuron (2017) 935: 1138-1152.e6. 129 202 WB; tested species: mouse

A model for regulation by SynGAP-α1 of binding of synaptic proteins to PDZ-domain 'Slots' in the postsynaptic density.
Walkup WG, Mastro TL, Schenker LT, Vielmetter J, Hu R, Iancu A, Reghunathan M, Bannon BD, Kennedy MB
eLife (2016) 5: . 129 202 WB; tested species: mouse

GABA(A) receptors can initiate the formation of functional inhibitory GABAergic synapses.
Fuchs C, Abitbol K, Burden JJ, Mercer A, Brown L, Iball J, Anne Stephenson F, Thomson AM, Jovanovic JN
The European journal of neuroscience (2013) 388: 3146-58. 129 202 WB; tested species: rat

An unexpected role of neuroligin-2 in regulating KCC2 and GABA functional switch.
Sun C, Zhang L, Chen G
Molecular brain (2013) 6: 23. 129 202 WB

PSD-95 deficiency alters GABAergic inhibition in the prefrontal cortex.
McEachern EP, Coley AA, Yang SS, Gao WJ
Neuropharmacology (2020) 179: 108277. 129 202 WB, IP; tested species: mouse

Cat. No.: 129 202

Amount: 200 µl

Price: $360.00

PSD-95 deficiency alters GABAergic inhibition in the prefrontal cortex.
McEachern EP, Coley AA, Yang SS, Gao WJ
Neuropharmacology (2020) 179: 108277. 129 202 WB, IP; tested species: mouse

SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling.
Halff EF, Szulc BR, Lesept F, Kittler JT
Cell reports (2019) 299: 2599-2607.e6. 129 202 WB; tested species: rat

Assembly rules for GABAA receptor complexes in the brain.
Martenson JS, Yamasaki T, Chaudhury NH, Albrecht D, Tomita S
eLife (2017) 6: . 129 202 WB; tested species: mouse

An unexpected role of neuroligin-2 in regulating KCC2 and GABA functional switch.
Sun C, Zhang L, Chen G
Molecular brain (2013) 6: 23. 129 202 WB

PSD-95 deficiency alters GABAergic inhibition in the prefrontal cortex.
McEachern EP, Coley AA, Yang SS, Gao WJ
Neuropharmacology (2020) 179: 108277. 129 202 WB, IP; tested species: mouse

Background

Neuroligins form a family of postsynaptic cell surface molecules that interact with β-neurexins. They are 110-120 kDa polypeptides with homology to acetylcholine esterase. Neuroligin1 and neuroligin3 are specifically localized to post-synaptic densities of excitatory synapses whereas neuroligin2 is found exclusively on inhibitory synapses.
Mutations in neuroligin3 and neuroligin4 have been implicated with a rare, heritable form of autism.

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2 ) Missler M et al., J. Neurochem. (1998)
3 ) Ichtchenko K et al., J. Biol. Chem. (1996)
4 ) Ichtchenko K et al., Cell (1995)
5 ) Südhof TC et al., Nature (1995)
6 ) Chubykin AA et al., Neuron (2007)
7 ) Chubykin AA et al., J. Biol. Chem. (2005)
8 ) Varoqueaux F et al., Eur. J. Cell Biol. (2004)
9 ) Dresbach T et al., Mol. Cell. Neurosci. (2004)

Protocols

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Neuroligin2 antibody - 129 202 K.O.

References for Neuroligin2 - 129 202