Synaptic Systems - Neuroligin3

Cat. No.: 129 113

Amount: 50 µg

Price: $380.00

Cat. No. 129 113	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: yes gallery IHC: not tested yet IHC-P: 1 : 1000 up to 1 : 2500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse Neuroligin 3 (UniProt Id: Q8BYM5)
Reactivity	Reacts with: rat (Q62889), mouse (Q8BYM5). No signal: zebrafish. Other species not tested yet.
Specificity	K.O. validated PubMed: 31002480
Matching control protein/peptide	129-3P
Data sheet	129_113.pdf

References for Neuroligin3 - 129 113

Key roles of C2/GAP domains in SYNGAP1-related pathophysiology.
Katsanevaki D, Till SM, Buller-Peralta I, Nawaz MS, Louros SR, Kapgal V, Tiwari S, Walsh D, Anstey NJ, Petrović NG, Cormack A, et al.
Cell reports (2024) 439: 114733. 129 113 WB; tested species: rat

Neuroligin-3 and Neuroligin-4X form Nanoscopic clusters and regulate growth cone organization and size.
Gatford NJF, Deans PJM, Duarte RRR, Chennell G, Sellers KJ, Raval P, Srivastava DP
Human molecular genetics (2021) : . 129 113 WB, ICC; tested species: mouse

TSPAN5 Enriched Microdomains Provide a Platform for Dendritic Spine Maturation through Neuroligin-1 Clustering.
Moretto E, Longatti A, Murru L, Chamma I, Sessa A, Zapata J, Hosy E, Sainlos M, Saint-Pol J, Rubinstein E, Choquet D, et al.
Cell reports (2019) 295: 1130-1146.e8. 129 113 WB; tested species: mouse

Colonic dilation and altered ex vivo gastrointestinal motility in the neuroligin-3 knockout mouse.
Leembruggen AJL, Balasuriya GK, Zhang J, Schokman S, Swiderski K, Bornstein JC, Nithianantharajah J, Hill-Yardin EL
Autism research : official journal of the International Society for Autism Research (2019) : . 129 113 WB, IP; KO verified; tested species: mouse

Neuroligin 1, 2, and 3 Regulation at the Synapse: FMRP-Dependent Translation and Activity-Induced Proteolytic Cleavage.
Chmielewska JJ, Kuzniewska B, Milek J, Urbanska K, Dziembowska M
Molecular neurobiology (2018) : . 129 113 WB; tested species: mouse

UPR activation specifically modulates glutamate neurotransmission in the cerebellum of a mouse model of autism.
Trobiani L, Favaloro FL, Di Castro MA, Di Mattia M, Cariello M, Miranda E, Canterini S, De Stefano ME, Comoletti D, Limatola C, De Jaco A, et al.
Neurobiology of disease (2018) : . 129 113 WB; tested species: mouse

Isoform-specific cleavage of neuroligin-3 reduces synapse strength.
Bemben MA, Nguyen TA, Li Y, Wang T, Nicoll RA, Roche KW
Molecular psychiatry (2018) : . 129 113 WB; tested species: rat

A unique intracellular tyrosine in neuroligin-1 regulates AMPA receptor recruitment during synapse differentiation and potentiation.
Letellier M, Szíber Z, Chamma I, Saphy C, Papasideri I, Tessier B, Sainlos M, Czöndör K, Thoumine O
Nature communications (2018) 91: 3979. 129 113 WB, IP; tested species: rat

Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.
Li J, Han W, Pelkey KA, Duan J, Mao X, Wang YX, Craig MT, Dong L, Petralia RS, McBain CJ, Lu W, et al.
Neuron (2017) 964: 808-826.e8. 129 113 WB; tested species: mouse

Dysfunctional cerebellar Purkinje cells contribute to autism-like behaviour in Shank2-deficient mice.
Peter S, Ten Brinke MM, Stedehouder J, Reinelt CM, Wu B, Zhou H, Zhou K, Boele HJ, Kushner SA, Lee MG, Schmeisser MJ, et al.
Nature communications (2016) 7: 12627. 129 113 WB; tested species: mouse

Cat. No.: 129 113

Amount: 50 µg

Price: $380.00

Isoform-specific cleavage of neuroligin-3 reduces synapse strength.
Bemben MA, Nguyen TA, Li Y, Wang T, Nicoll RA, Roche KW
Molecular psychiatry (2018) : . 129 113 WB; tested species: rat

ICC

IHC-P

Background

Neuroligins form a family of post-synaptic cell surface molecules that interact with β-neurexins. They are 110-120 kDa polypeptides with homology to acetylcholine esterase. Neuroligin1 and neuroligin3 are specifically localized to post-synaptic densities of excitatory synapses whereas neuroligin2 is found exclusively on inhibitory synapses.
Mutations in neuroligin3 and neuroligin4 have been implicated with a rare, heritable form of autism.

1 ) Song JY et al., Proc. Natl. Acad. Sci. U.S.A. (1999)
2 ) Missler M et al., J. Neurochem. (1998)
3 ) Ichtchenko K et al., J. Biol. Chem. (1996)
4 ) Ichtchenko K et al., Cell (1995)
5 ) Südhof TC et al., Nature (1995)
6 ) Chubykin AA et al., Neuron (2007)
7 ) Chubykin AA et al., J. Biol. Chem. (2005)
8 ) Varoqueaux F et al., Eur. J. Cell Biol. (2004)
9 ) Dresbach T et al., Mol. Cell. Neurosci. (2004)

Protocols

Standard protocol

ICC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Neuroligin3 antibody - 129 113 K.O.

References for Neuroligin3 - 129 113