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Home > Products > 128 002

alpha/beta Synuclein antibody - 128 002

Synucleins are involved in Parkinson's and Alzheimer's Disease
Rabbit polyclonal antiserum
Cat. No.: 128 002
Amount: 200 µl
Price: $355.00
Cat. No. 128 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 1000 up to 1 : 2000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 2 to 25 from human α-Synuclein (UniProt Id: P37840)
Reactivity Reacts with: rat (P37377, Q63754), mouse (O55042, Q91ZZ3), zebrafish, human (P37840, Q16143).
Other species not tested yet.
Specificity Recognizes α synuclein and β synuclein.
Data sheet 128_002.pdf

References for alpha/beta Synuclein - 128 002

DOPAL initiates αSynuclein-dependent impaired proteostasis and degeneration of neuronal projections in Parkinson's disease.
Masato A, Plotegher N, Terrin F, Sandre M, Faustini G, Thor A, Adams S, Berti G, Cogo S, De Lazzari F, Fontana CM, et al.
NPJ Parkinson's disease (2023) 91: 42. 128 002 WB; tested species: mouse,rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 128 002 WB, ICC, IHC; tested species: mouse,rat
Over-expression of alpha-synuclein in the nervous system enhances axonal degeneration after peripheral nerve lesion in a transgenic mouse strain.
Siebert H, Kahle PJ, Kramer ML, Isik T, Schlüter OM, Schulz-Schaeffer WJ, Brück W
Journal of neurochemistry (2010) 1144: 1007-18. 128 002 WB
Brain alpha-synuclein accumulation in multiple system atrophy, Parkinson's disease and progressive supranuclear palsy: a comparative investigation.
Tong J, Wong H, Guttman M, Ang LC, Forno LS, Shimadzu M, Rajput AH, Muenter MD, Kish SJ, Hornykiewicz O, Furukawa Y, et al.
Brain : a journal of neurology (2010) 133Pt 1: 172-88. 128 002 WB; tested species: human
Mitochondrial translocation of alpha-synuclein is promoted by intracellular acidification.
Cole NB, Dieuliis D, Leo P, Mitchell DC, Nussbaum RL
Experimental cell research (2008) 31410: 2076-89. 128 002 WB, ICC, EM
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 128 002 WB
alpha-Synuclein protects SH-SY5Y cells from dopamine toxicity.
Colapinto M, Mila S, Giraudo S, Stefanazzi P, Molteni M, Rossetti C, Bergamasco B, Lopiano L, Fasano M
Biochemical and biophysical research communications (2006) 3494: 1294-300. 128 002 WB
Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein.
Steinacker P, Schwarz P, Reim K, Brechlin P, Jahn O, Kratzin H, Aitken A, Wiltfang J, Aguzzi A, Bahn E, Baxter HC, et al.
Molecular and cellular biology (2005) 254: 1339-46. 128 002 WB
A broken alpha -helix in folded alpha -Synuclein.
Chandra S, Chen X, Rizo J, Jahn R, Südhof TC
The Journal of biological chemistry (2003) 27817: 15313-8. 128 002 WB
Trafficking proteins show limited differences in mobility across different postsynaptic spines.
Mougios N, Opazo F, Rizzoli SO, Reshetniak S
iScience (2023) 262: 105971. 128 002 ICC; tested species: rat
A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol.
Gerdes C, Waal N, Offner T, Fornasiero EF, Wender N, Verbarg H, Manzini I, Trenkwalder C, Mollenhauer B, Strohäker T, Zweckstetter M, et al.
Nature communications (2020) 111: 2729. 128 002 ICC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 128 002 WB, ICC, IHC; tested species: mouse,rat
Mitochondrial translocation of alpha-synuclein is promoted by intracellular acidification.
Cole NB, Dieuliis D, Leo P, Mitchell DC, Nussbaum RL
Experimental cell research (2008) 31410: 2076-89. 128 002 WB, ICC, EM
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 128 002 WB, ICC, IHC; tested species: mouse,rat
One-step nanoscale expansion microscopy reveals individual protein shapes.
Shaib AH, Chouaib AA, Chowdhury R, Altendorf J, Mihaylov D, Zhang C, Krah D, Imani V, Spencer RKW, Georgiev SV, Mougios N, et al.
Nature biotechnology (2024) : . 128 002 ExM; tested species: rat
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 128 002 DNA-PAINT; tested species: rat
Mitochondrial translocation of alpha-synuclein is promoted by intracellular acidification.
Cole NB, Dieuliis D, Leo P, Mitchell DC, Nussbaum RL
Experimental cell research (2008) 31410: 2076-89. 128 002 WB, ICC, EM
Cat. No.: 128 002
Amount: 200 µl
Price: $355.00
DOPAL initiates αSynuclein-dependent impaired proteostasis and degeneration of neuronal projections in Parkinson's disease.
Masato A, Plotegher N, Terrin F, Sandre M, Faustini G, Thor A, Adams S, Berti G, Cogo S, De Lazzari F, Fontana CM, et al.
NPJ Parkinson's disease (2023) 91: 42. 128 002 WB; tested species: mouse,rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 128 002 WB, ICC, IHC; tested species: mouse,rat
Over-expression of alpha-synuclein in the nervous system enhances axonal degeneration after peripheral nerve lesion in a transgenic mouse strain.
Siebert H, Kahle PJ, Kramer ML, Isik T, Schlüter OM, Schulz-Schaeffer WJ, Brück W
Journal of neurochemistry (2010) 1144: 1007-18. 128 002 WB
Brain alpha-synuclein accumulation in multiple system atrophy, Parkinson's disease and progressive supranuclear palsy: a comparative investigation.
Tong J, Wong H, Guttman M, Ang LC, Forno LS, Shimadzu M, Rajput AH, Muenter MD, Kish SJ, Hornykiewicz O, Furukawa Y, et al.
Brain : a journal of neurology (2010) 133Pt 1: 172-88. 128 002 WB; tested species: human
Mitochondrial translocation of alpha-synuclein is promoted by intracellular acidification.
Cole NB, Dieuliis D, Leo P, Mitchell DC, Nussbaum RL
Experimental cell research (2008) 31410: 2076-89. 128 002 WB, ICC, EM
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 128 002 WB
alpha-Synuclein protects SH-SY5Y cells from dopamine toxicity.
Colapinto M, Mila S, Giraudo S, Stefanazzi P, Molteni M, Rossetti C, Bergamasco B, Lopiano L, Fasano M
Biochemical and biophysical research communications (2006) 3494: 1294-300. 128 002 WB
Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein.
Steinacker P, Schwarz P, Reim K, Brechlin P, Jahn O, Kratzin H, Aitken A, Wiltfang J, Aguzzi A, Bahn E, Baxter HC, et al.
Molecular and cellular biology (2005) 254: 1339-46. 128 002 WB
A broken alpha -helix in folded alpha -Synuclein.
Chandra S, Chen X, Rizo J, Jahn R, Südhof TC
The Journal of biological chemistry (2003) 27817: 15313-8. 128 002 WB
Trafficking proteins show limited differences in mobility across different postsynaptic spines.
Mougios N, Opazo F, Rizzoli SO, Reshetniak S
iScience (2023) 262: 105971. 128 002 ICC; tested species: rat
A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol.
Gerdes C, Waal N, Offner T, Fornasiero EF, Wender N, Verbarg H, Manzini I, Trenkwalder C, Mollenhauer B, Strohäker T, Zweckstetter M, et al.
Nature communications (2020) 111: 2729. 128 002 ICC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 128 002 WB, ICC, IHC; tested species: mouse,rat
Mitochondrial translocation of alpha-synuclein is promoted by intracellular acidification.
Cole NB, Dieuliis D, Leo P, Mitchell DC, Nussbaum RL
Experimental cell research (2008) 31410: 2076-89. 128 002 WB, ICC, EM
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 128 002 WB, ICC, IHC; tested species: mouse,rat
One-step nanoscale expansion microscopy reveals individual protein shapes.
Shaib AH, Chouaib AA, Chowdhury R, Altendorf J, Mihaylov D, Zhang C, Krah D, Imani V, Spencer RKW, Georgiev SV, Mougios N, et al.
Nature biotechnology (2024) : . 128 002 ExM; tested species: rat
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 128 002 DNA-PAINT; tested species: rat
Mitochondrial translocation of alpha-synuclein is promoted by intracellular acidification.
Cole NB, Dieuliis D, Leo P, Mitchell DC, Nussbaum RL
Experimental cell research (2008) 31410: 2076-89. 128 002 WB, ICC, EM
WB
ICC
IHC
IHC-P
Background
Synuclein proteins are produced by three genes. They share structural resemblance to apolipoproteins, but are abundant in the neuronal cytosol and present in enriched amounts at presynaptic terminals.
Synucleins have been specifically implicated in three diseases: Alzheimer's (AD), Parkinson's (PD) and breast cancer. In AD, a peptide derived from α-synuclein forms an intrinsic component of plaque amyloid. In PD, an α-synuclein allele is genetically linked to several independent familial cases, and the protein appears to accumulate in Lewy bodies. In breast cancer, increased expression of γ-synuclein correlates with disease progression.
In songbirds, α-synuclein expression is correlated with plasticity in the developing song control system. Although the normal function of synucleins is unknown, a role in synaptic plasticity seems likely.
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