Synaptic Systems

Cat. No.: 124 302

Amount: 200 µl

Price: $360.00

Cat. No. 124 302	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 115 to 133 from rat SAP97 (UniProt Id: Q62696)
Reactivity	Reacts with: rat (Q62696). No signal: zebrafish. Other species not tested yet.
Matching control protein/peptide	124-3P
Data sheet	Datasheet 124_302

References for SAP97 - 124 302

Myosin VI Drives Clathrin-Mediated AMPA Receptor Endocytosis to Facilitate Cerebellar Long-Term Depression.
Wagner W, Lippmann K, Heisler FF, Gromova KV, Lombino FL, Roesler MK, Pechmann Y, Hornig S, Schweizer M, Polo S, Schwarz JR, et al.
Cell reports (2019) 281: 11-20.e9. 124 302 WB; tested species: mouse

Altered postsynaptic-density-levels of caldendrin in the para-chloroamphetamine-induced serotonin syndrome but not in the rat ketamine model of psychosis.
Smalla KH, Sahin J, Putzke J, Tischmeyer W, Gundelfinger ED, Kreutz MR
Neurochemical research (2009) 348: 1405-9. 124 302 WB

Cat. No.: 124 302

Amount: 200 µl

Price: $360.00

Background

SAP 97 (synapse associated protein of 97 kDa, also called DLG 1) like PSD 95 and SAP 102, is a scaffolding protein of the postsynaptic density. It consists of three type-one PDZ domains and binds selectively to the C-terminus of the GluR1 subunit of AMPA receptors.
SAP 97 is probably involved in the clustering of AMPA receptors at the post-synaptic membrane.
It is also expressed in heart, where it has been shown to be involved in the targeting of voltage gated potassium channels to subpopulations of lipid rafts.

1 ) Cai C et al., J. Biol. Chem. (2006)
2 ) Folco EJ et al., Am. J. Physiol. Heart Circ. Physiol. (2004)
3 ) Leonoudakis D et al., J. Biol. Chem. (2004)
4 ) Gardoni F et al., J. Biol. Chem. (2003)
5 ) Mehta S et al., J. Biol. Chem. (2001)
6 ) Valtschanoff JG et al., Eur. J. Neurosci. (2000)
7 ) Tiffany AM et al., J. Cell Biol. (2000)
8 ) Bassand P et al., Eur. J. Neurosci. (1999)
9 ) Leonard AS et al., J. Biol. Chem. (1998)

Protocols

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SAP97 antibody - 124 302

References for SAP97 - 124 302