Complexin1/2 antibody SNARE binding domain - 122 121

Complexins are nerve-terminal syntaxin binding proteins

Mouse monoclonal hybridoma supernatant

Cat. No.: 122 121

Amount: 100 µl

Price: $425.00

Cat. No. 122 121	100 µl hybridoma supernatant, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 up to 1 : 500 gallery IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	314C5
Subtype	IgM (κ light chain)
Immunogen	Recombinant protein corresponding to AA 43 to 91 from mouse Complexin2 (UniProt Id: P84086)
Reactivity	Reacts with: rat (P63041, P84087), mouse (P63040, P84086). Other species not tested yet.
Specificity	Recognized complexin 1 and 2
Data sheet	Datasheet 122_121

Cat. No.: 122 121

Amount: 100 µl

Price: $425.00

ICC

Background

Complexins are enriched in neurons where they colocalize with syntaxin 1 and SNAP 25. In addition, complexin 2, also referred to as synaphin 1, is expressed ubiquitously at low levels. Complexins bind weakly to syntaxin 1 alone and not at all to synaptobrevin and SNAP 25, but strongly to the SNAP receptor-core complex composed of these three molecules. They compete with α-SNAP for binding to the core complex but not with other interacting molecules, suggesting that complexins regulate the sequential interactions of α-SNAP and synaptotagmins with the SNAP receptor during exocytosis.
In retinal ribbon synapses complexin 3 and complexin 4 functionally replace complexin 1 (synaphin 2) and 2. They have similar biochemical binding properties and are farnesylated at their C-terminus.

1 ) Südhof TC et al., Nature (1995)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) McMahon HT et al., Cell (1995)

Protocols

ICC

Fixed Cells

Other products for this target