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Amphiphysin - 120 003

Nerve-terminal protein binding clathrin, dynamin, and synaptojanin
Polyclonal rabbit purified antibody
Cat. No.: 120 003
Amount: 50 µg
Price: $450.00
Cat. No. 120 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Immunogen Synthetic peptide corresponding to AA 2 to 15 from rat Amphiphysin (UniProt Id: O08838)
Reactivity Reacts with: human (P49418), rat (O08838), mouse (Q7TQF7), hamster, cow, chicken.
Other species not tested yet.
Specificity Specific for amphiphysin.
Matching control protein/peptide 120-0P
Data sheet 120_003.pdf
Cat. No.: 120 003
Quantity: 50 µg
Price: $450.00
Background

Amphiphysin, a dominant autoantigen in paraneoplastic Stiff-man syndrome, is a neuronal protein highly concentrated in nerve terminals. It is an abundant presynaptic protein that interacts via its COOH-terminal src homology 3 (SH3) domain with the GTPase dynamin and the inositol-5-phosphatase synaptojanin and additionally directly to clathrin.
Amphiphysin, dynamin and synaptojanin have a putative role in synaptic vesicle recycling and undergo rapid dephosphorylation in synaptosomes stimulated by a depolarizing stimulus. Two isoforms are known which seem to act in concert as a heterodimer.