Synaptic Systems

Cat. No.: 119 011

Amount: 100 µg

Price: $415.00

Cat. No. 119 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 100 up to 1 : 500 gallery IHC: not recommended IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	17G10
Subtype	IgG3 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of human SV2 A (UniProt Id: Q7L0J3)
Reactivity	Reacts with: human (Q7L0J3), rat (Q02563), mouse (Q9JIS5). Other species not tested yet.
Matching control protein/peptide	119-0P
Remarks	WB: SV2 A aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	119_011.pdf

References for SV2 A - 119 011

Vesicular Glutamate Release from Feeder-FreehiPSC-Derived Neurons.
Baldassari S, Cervetto C, Amato S, Fruscione F, Balagura G, Pelassa S, Musante I, Iacomino M, Traverso M, Corradi A, Scudieri P, et al.
International journal of molecular sciences (2022) 2318: . 119 011 WB, ICC; tested species: human

Botulinum Neurotoxins A, B, C, E, and F preferentially enter cultured human motor neurons compared to other cultured human neuronal populations.
Pellett S, Tepp WH, Johnson EA
FEBS letters (2019) : . 119 011 WB; tested species: human

Tuning of glutamate, but not GABA, release by an intra-synaptic vesicles APP domain whose function can be modulated by β- or α-secretase cleavage.
Yao W, Tambini MD, Liu X, D'Adamio L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 119 011 WB; tested species: mouse

Protective Role of Levetiracetam Against Cognitive Impairment And Brain White Matter Damage in Mouse prolonged Cerebral Hypoperfusion.
Inaba T, Miyamoto N, Hira K, Ueno Y, Yamashiro K, Watanabe M, Shimada Y, Hattori N, Urabe T
Neuroscience (2019) 414: 255-264. 119 011 WB; tested species: mouse

Ubiquitin-Synaptobrevin Fusion Protein Causes Degeneration of Presynaptic Motor Terminals in Mice.
Liu Y, Li H, Sugiura Y, Han W, Gallardo G, Khvotchev M, Zhang Y, Kavalali ET, Südhof TC, Lin W
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3533: 11514-31. 119 011 WB

A sensitive cell-based assay for testing potency of Botulinum neurotoxin type A.
Caliskan C, Simsek D, Leese C, Doran C, Seward E, Peden AA, Davletov B
ALTEX (2024) : . 119 011 ICC; tested species: human

Glycosylation is dispensable for sorting of synaptotagmin 1 but is critical for targeting of SV2 and synaptophysin to recycling synaptic vesicles.
Kwon SE, Chapman ER
The Journal of biological chemistry (2012) 28742: 35658-68. 119 011 ICC

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 119 011 DNA-PAINT; tested species: rat

Cat. No.: 119 011

Amount: 100 µg

Price: $415.00

ICC

IHC-P

Background

SV2s (synaptic vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2 A, SV2 B and SV2 C. SV2 A is expressed ubiquitously throughout the brain and is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium- stimulated exocytosis. SV2 B has a more restricted distribution with varying degrees of coexpression with SV2 A. SV2 C is more closely related to SV2 A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2 expression has also been observed in other organs. In kidney it localizes to podocytes.

1 ) Xu T et al., Nat. Cell Biol. (2001)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Fernández-Chacón R et al., Annu. Rev. Physiol. (1999)
4 ) Südhof TC et al., Nature (1995)
5 ) Bajjalieh SM et al., J. Neurosci. (1994)
6 ) Bajjalieh SM et al., Science (1992)
7 ) Feany MB et al., Cell (1992)

Protocols

Standard protocol

ICC

IHC-P

OTHER

Preabsorbtion

Other products for this target

SV2 A antibody - 119 011

References for SV2 A - 119 011