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Home > Products > 119 004

SV2 A antibody - 119 004

SV2s are highly glycosylated synaptic vesicle proteins with homology to transmembrane transporters
Guinea pig polyclonal antiserum
Cat. No.: 119 004
Amount: 100 µl
Price: $365.00
Cat. No. 119 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 200 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to residues near the amino terminus of human SV2 A (UniProt Id: Q7L0J3)
Reactivity Reacts with: human (Q7L0J3), rat (Q02563), mouse (Q9JIS5).
Other species not tested yet.
Matching control protein/peptide 119-0P
Remarks

WB:  SV2 A aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 119_004.pdf

References for SV2 A - 119 004

Axonal Transport of Lysosomes Is Unaffected in Glucocerebrosidase-Inhibited iPSC-Derived Forebrain Neurons.
Keefe AJ, Gabrych DR, Zhu Y, Vocadlo DJ, Silverman MA
eNeuro (2023) 1010: . 119 004 ICC; tested species: human
Presynapses contain distinct actin nanostructures.
Bingham D, Jakobs CE, Wernert F, Boroni-Rueda F, Jullien N, Schentarra EM, Friedl K, Da Costa Moura J, van Bommel DM, Caillol G, Ogawa Y, et al.
The Journal of cell biology (2023) 22210: . 119 004 ICC; tested species: rat
Wide-field diffuse amacrine cells in the monkey retina contain immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART).
Long Y, Bordt AS, Liu WS, Davis EP, Lee SJ, Tseng L, Chuang AZ, Whitaker CM, Massey SC, Sherman MB, Marshak DW, et al.
Peptides (2016) 84: 22-35. 119 004 IHC; tested species: monkey
Cat. No.: 119 004
Amount: 100 µl
Price: $365.00
Axonal Transport of Lysosomes Is Unaffected in Glucocerebrosidase-Inhibited iPSC-Derived Forebrain Neurons.
Keefe AJ, Gabrych DR, Zhu Y, Vocadlo DJ, Silverman MA
eNeuro (2023) 1010: . 119 004 ICC; tested species: human
Presynapses contain distinct actin nanostructures.
Bingham D, Jakobs CE, Wernert F, Boroni-Rueda F, Jullien N, Schentarra EM, Friedl K, Da Costa Moura J, van Bommel DM, Caillol G, Ogawa Y, et al.
The Journal of cell biology (2023) 22210: . 119 004 ICC; tested species: rat
Wide-field diffuse amacrine cells in the monkey retina contain immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART).
Long Y, Bordt AS, Liu WS, Davis EP, Lee SJ, Tseng L, Chuang AZ, Whitaker CM, Massey SC, Sherman MB, Marshak DW, et al.
Peptides (2016) 84: 22-35. 119 004 IHC; tested species: monkey
WB
ICC
IHC
IHC
IHC
IHC-P
Background

SV2s (synaptic vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2 A, SV2 B and SV2 C. SV2 A is expressed ubiquitously throughout the brain and is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium- stimulated exocytosis. SV2 B has a more restricted distribution with varying degrees of coexpression with SV2 A. SV2 C is more closely related to SV2 A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2 expression has also been observed in other organs. In kidney it localizes to podocytes.
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