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Home > Products > 119 003

SV2 A antibody - 119 003

SV2s are highly glycosylated synaptic vesicle proteins with homology to transmembrane transporters
Rabbit polyclonal purified antibody
Cat. No.: 119 003
Amount: 50 µg
Price: $455.00
Cat. No. 119 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to residues near the amino terminus of human SV2 A (UniProt Id: Q7L0J3)
Reactivity Reacts with: human (Q7L0J3), rat (Q02563), mouse (Q9JIS5), Guinea pig, cow, hamster, chicken, zebrafish.
Other species not tested yet.
Matching control protein/peptide 119-0P
Remarks

WB: SV2 A aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 119_003.pdf

References for SV2 A - 119 003

Human-Relevant Sensitivity of iPSC-Derived Human Motor Neurons to BoNT/A1 and B1.
Schenke M, Prause HC, Bergforth W, Przykopanski A, Rummel A, Klawonn F, Seeger B
Toxins (2021) 138: . 119 003 WB; tested species: human
Vesicular Glutamate Transporters (SLCA17 A6, 7, 8) Control Synaptic Phosphate Levels.
Cheret C, Ganzella M, Preobraschenski J, Jahn R, Ahnert-Hilger G
Cell reports (2021) 342: 108623. 119 003 WB; tested species: mouse
Rapid purification and metabolomic profiling of synaptic vesicles from mammalian brain.
Chantranupong L, Saulnier JL, Wang W, Jones DR, Pacold ME, Sabatini BL
eLife (2020) 9: . 119 003 WB; tested species: mouse
Reductions in Synaptic Vesicle Glycoprotein 2 Isoforms in the Cortex and Hippocampus in a Rat Model of Traumatic Brain Injury.
Fronczak KM, Li Y, Henchir J, Dixon CE, Carlson SW
Molecular neurobiology (2021) : . 119 003 IHC; tested species: rat
Microglial dyshomeostasis drives perineuronal net and synaptic loss in a CSF1R+/- mouse model of ALSP, which can be rescued via CSF1R inhibitors.
Arreola MA, Soni N, Crapser JD, Hohsfield LA, Elmore MRP, Matheos DP, Wood MA, Swarup V, Mortazavi A, Green KN
Science advances (2021) 735: . 119 003 IHC; tested species: mouse
Cat. No.: 119 003
Amount: 50 µg
Price: $455.00
Human-Relevant Sensitivity of iPSC-Derived Human Motor Neurons to BoNT/A1 and B1.
Schenke M, Prause HC, Bergforth W, Przykopanski A, Rummel A, Klawonn F, Seeger B
Toxins (2021) 138: . 119 003 WB; tested species: human
Vesicular Glutamate Transporters (SLCA17 A6, 7, 8) Control Synaptic Phosphate Levels.
Cheret C, Ganzella M, Preobraschenski J, Jahn R, Ahnert-Hilger G
Cell reports (2021) 342: 108623. 119 003 WB; tested species: mouse
Rapid purification and metabolomic profiling of synaptic vesicles from mammalian brain.
Chantranupong L, Saulnier JL, Wang W, Jones DR, Pacold ME, Sabatini BL
eLife (2020) 9: . 119 003 WB; tested species: mouse
Reductions in Synaptic Vesicle Glycoprotein 2 Isoforms in the Cortex and Hippocampus in a Rat Model of Traumatic Brain Injury.
Fronczak KM, Li Y, Henchir J, Dixon CE, Carlson SW
Molecular neurobiology (2021) : . 119 003 IHC; tested species: rat
Microglial dyshomeostasis drives perineuronal net and synaptic loss in a CSF1R+/- mouse model of ALSP, which can be rescued via CSF1R inhibitors.
Arreola MA, Soni N, Crapser JD, Hohsfield LA, Elmore MRP, Matheos DP, Wood MA, Swarup V, Mortazavi A, Green KN
Science advances (2021) 735: . 119 003 IHC; tested species: mouse
WB
ICC
IHC
IHC-P
Background

SV2s (synaptic vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2 A, SV2 B and SV2 C. SV2 A is expressed ubiquitously throughout the brain and is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium- stimulated exocytosis. SV2 B has a more restricted distribution with varying degrees of coexpression with SV2 A. SV2 C is more closely related to SV2 A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2 expression has also been observed in other organs. In kidney it localizes to podocytes.
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