IP3-receptor type1 antibody - 117 003

IP3-receptor triggers the release of Ca²⁺ from intracellular stores

Rabbit polyclonal purified antibody

Cat. No.: 117 003

Amount: 50 µg

Price: $455.00

Cat. No. 117 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 2731 to 2749 from rat IP3-receptortype1 (UniProt Id: P29994)
Reactivity	Reacts with: human (Q14643), rat (P29994), mouse (P11881), cow. Other species not tested yet.
Matching control protein/peptide	117-0P
Remarks	WB: Due to its large size, IP3 receptor requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
Data sheet	117_003.pdf

References for IP3-receptor type1 - 117 003

Disruption of endoplasmic reticulum-mitochondria tethering proteins in post-mortem Alzheimer's disease brain.
Lau DHW, Paillusson S, Hartopp N, Rupawala H, Mórotz GM, Gomez-Suaga P, Greig J, Troakes C, Noble W, Miller CCJ
Neurobiology of disease (2020) : 105020. 117 003 WB; tested species: human

Stimulating VAPB-PTPIP51 ER-mitochondria tethering corrects FTD/ALS mutant TDP43 linked Ca2+ and synaptic defects.
Markovinovic A, Martín-Guerrero SM, Mórotz GM, Salam S, Gomez-Suaga P, Paillusson S, Greig J, Lee Y, Mitchell JC, Noble W, Miller CCJ, et al.
Acta neuropathologica communications (2024) 121: 32. 117 003 ICC; tested species: human

Cat. No.: 117 003

Amount: 50 µg

Price: $455.00

IHC

IHC-P

Background

Inositol 1, 4, 5 - trisphophate InsP3 is an intracellular messenger that triggers release of Ca²⁺ from intracellular stores. InsP3 acts by binding to specific receptors localized to endoplasmic reticulum.
There are at least three types of InsP3 receptors, of which the type 1 receptor is the most abundant. All three receptors appear to be widely expressed. Highest levels of the type 1 InsP3 receptors are found in neurons, with very high expression in the Purkinje cells of the cerebellum.

1 ) Dawson AP et al., Curr. Biol. (1997)
2 ) Südhof TC et al., EMBO J. (1991)
3 ) Mignery GA et al., Nature (1989)
4 ) Furuichi T et al., Nature (1989)

Protocols

IHC

IHC-P

OTHER

Preabsorbtion

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