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Home > Products > 117 002

IP3-receptor type1 antibody - 117 002

IP3-receptor triggers the release of Ca2+ from intracellular stores
Rabbit polyclonal antiserum
Cat. No.: 117 002
Amount: 200 µl
Price: $360.00
Cat. No. 117 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: 1 : 100 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 2731 to 2749 from rat IP3-receptortype1 (UniProt Id: P29994)
Reactivity Reacts with: human (Q14643), rat (P29994), mouse (P11881), cow.
Other species not tested yet.
Matching control protein/peptide 117-0P
Remarks

WB: Due to its large size, IP3 receptor requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
Data sheet 117_002.pdf

References for IP3-receptor type1 - 117 002

The IP3 R Binding Protein Released With Inositol 1,4,5-Trisphosphate Is Expressed in Rodent Reproductive Tissue and Spermatozoa.
Borth H, Weber N, Meyer D, Wartenberg A, Arlt E, Zierler S, Breit A, Wennemuth G, Gudermann T, Boekhoff I
Journal of cellular physiology (2016) 2315: 1114-29. 117 002 WB
The proteome of the presynaptic active zone: from docked synaptic vesicles to adhesion molecules and maxi-channels.
Morciano M, Beckhaus T, Karas M, Zimmermann H, Volknandt W
Journal of neurochemistry (2009) 1083: 662-75. 117 002 WB
INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 117 002 IHC; tested species: human,mouse
Cat. No.: 117 002
Amount: 200 µl
Price: $360.00
The IP3 R Binding Protein Released With Inositol 1,4,5-Trisphosphate Is Expressed in Rodent Reproductive Tissue and Spermatozoa.
Borth H, Weber N, Meyer D, Wartenberg A, Arlt E, Zierler S, Breit A, Wennemuth G, Gudermann T, Boekhoff I
Journal of cellular physiology (2016) 2315: 1114-29. 117 002 WB
The proteome of the presynaptic active zone: from docked synaptic vesicles to adhesion molecules and maxi-channels.
Morciano M, Beckhaus T, Karas M, Zimmermann H, Volknandt W
Journal of neurochemistry (2009) 1083: 662-75. 117 002 WB
INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 117 002 IHC; tested species: human,mouse
WB
IHC-P
Background
Inositol 1, 4, 5 - trisphophate InsP3 is an intracellular messenger that triggers release of Ca2+ from intracellular stores. InsP3 acts by binding to specific receptors localized to endoplasmic reticulum.
There are at least three types of InsP3 receptors, of which the type 1 receptor is the most abundant. All three receptors appear to be widely expressed. Highest levels of the type 1 InsP3 receptors are found in neurons, with very high expression in the Purkinje cells of the cerebellum.
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