Synaptic Systems - IP3-receptor type1

Cat. No.: 117 002

Amount: 200 µl

Price: $355.00

The IP3 R Binding Protein Released With Inositol 1,4,5-Trisphosphate Is Expressed in Rodent Reproductive Tissue and Spermatozoa.
Borth H, Weber N, Meyer D, Wartenberg A, Arlt E, Zierler S, Breit A, Wennemuth G, Gudermann T, Boekhoff I
Journal of cellular physiology (2016) 2315: 1114-29. 117 002 WB

The proteome of the presynaptic active zone: from docked synaptic vesicles to adhesion molecules and maxi-channels.
Morciano M, Beckhaus T, Karas M, Zimmermann H, Volknandt W
Journal of neurochemistry (2009) 1083: 662-75. 117 002 WB

WB

IHC-P

Background

Inositol 1, 4, 5 - trisphophate InsP3 is an intracellular messenger that triggers release of Ca²⁺ from intracellular stores. InsP3 acts by binding to specific receptors localized to endoplasmic reticulum.
There are at least three types of InsP3 receptors, of which the type 1 receptor is the most abundant. All three receptors appear to be widely expressed. Highest levels of the type 1 InsP3 receptors are found in neurons, with very high expression in the Purkinje cells of the cerebellum.

1 ) Dawson AP et al., Curr. Biol. (1997)
2 ) Südhof TC et al., EMBO J. (1991)
3 ) Mignery GA et al., Nature (1989)
4 ) Furuichi T et al., Nature (1989)

Protocols

WB

IHC-P

OTHER

Preabsorbtion

Other products for this target

Cat. No. 117 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: 1 : 100 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 2731 to 2749 from rat IP3-receptortype1 (UniProt Id: P29994)
Reactivity	Reacts with: human (Q14643), rat (P29994), mouse (P11881), cow. Other species not tested yet.
Matching control protein/peptide	117-0P
Remarks	WB: Due to its large size, IP3 receptor requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
Data sheet	117_002.pdf

IP3-receptor type1 antibody - 117 002

References for IP3-receptor type1 - 117 002