GluN1 antibody extracellular - 114 018

GluN1 is the major subunit of ionotropic NMDA type glutamate receptors

Rabbit monoclonal recombinant IgG

Cat. No.: 114 018

Amount: 50 µg

Price: $415.00

Cat. No. 114 018	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 (see remarks) gallery IHC: 1 : 500 (see remarks) gallery IHC-P: not recommended IHC-G: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence.
Clone	Rb003-102
Subtype	IgG1 (κ light chain)
Immunogen	Full length native human GluN1 (UniProt Id: Q05586)
Reactivity	Reacts with: mouse (P35438), rat (P35439). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal human antibody 003-102. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. ICC: This antibody is also suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents. IHC: Antibody works on PFA/formaldehyde tissue. For optimal signal to noise ratio, glyoxal fixation (IHC-G) is recommended. IHC-G: 9% glyoxal, 8% acetic acid, in ddH2O, pH 4.2-4.4, according to Konno et al. 2023 is recommended.
Data sheet	114_018.pdf

Cat. No.: 114 018

Amount: 50 µg

Price: $415.00

ICC

IHC

IHC-G

Background

GluNs (NMDA-receptors) represent a class of glutamate receptors that are of central importance in synaptic plasticity. Multiple NMDA receptor subtypes exist: GluN1 and GluN2 A-D. GluN1 is the most important as it is required for activity. NMDA-receptors allow Ca²⁺ influx and are thought to trigger Ca²⁺ dependent postsynaptic processes involved in long term potentiation and depression.

1 ) Nusser Z et al., Curr. Opin. Neurobiol. (2000)
2 ) Brose N et al., J. Biol. Chem. (1994)
3 ) Moriyoshi K et al., Nature (1991)
4 ) Collingridge GL et al., Neuropharmacology (2009)

Protocols

ICC

IHC

IHC-G

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