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SNAP 25 antibody - 111 308

SNAP25 is a palmitoylated plasma membrane protein; required for exocytosis
Guinea pig monoclonal recombinant IgG
Cat. No.: 111 308
Amount: 50 µg
Price: $415.00
Cat. No. 111 308 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: not tested yet
IHC-P/FFPE: 1 : 200 up to 1 : 1000 gallery  
Clone Gp71.1
Subtype IgG2 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 206 from rat SNAP25B (UniProt Id: P60881-1)
Epitop Epitop: AA 20 to 40 from rat SNAP25B (UniProt Id: P60881-1)
Reactivity Reacts with: human (P60880), rat (P60881-1), mouse (P60879), vertebrates, invertebrates, zebrafish.
Other species not tested yet.
Specificity Detects both splice variants SNAP 25A and B. Recognizes the Botulinum neurotoxin A and E cleavage products. K.O.
Remarks

This antibody is a chimeric antibody based on the well-known monoclonal mouse antibody clone 71.1. The constant regions of the heavy and light chains have been replaced by Guinea pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 111_308.pdf
Cat. No.: 111 308
Amount: 50 µg
Price: $415.00
Background

SNAP 25 (synaptosome-associated protein of 25 kDa) is a highly conserved protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP 25 is the target of Botulinum neurotoxin A and E which cleave off 9 and 26 amino acids, respectively, from the C-terminus.
SNAP 25 is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles with syntaxin 1 and synaptobrevin. It is abundantly localized on the neuronal plasmalemma and on recycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells. There are two splice-variants, SNAP 25A and 25B.