SNAP23 antibody - 111 203BT

SNAP23 is a palmitoylated plasma membrane protein; ubiquitously expressed isoform of SNAP 25

Rabbit polyclonal purified antibody

Cat. No.: 111 203BT

Amount: 50 µg

Price: $480.00

Cat. No. 111 203BT	50 µg specific antibody, lyophilized. Affinity purified with the immunogen, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes (see remarks) ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Immunogen	Synthetic peptide corresponding to AA 196 to 211 from human SNAP23 (UniProt Id: O00161)
Reactivity	Reacts with: rat (O70377), mouse (O09044). Other species not tested yet.
Matching control protein/peptide	111-2P
Remarks	IP: Antibody 2 (cat. no. 111 213) is recommended.
Data sheet	111_203bt.pdf

Cat. No.: 111 203BT

Amount: 50 µg

Price: $480.00

Background

SNAP23 (synaptosome-associated protein of 23 kDa) is an ubiquitously expressed isoform and functional homologue of SNAP25. It is resistant to cleavage by BoNT/A and E. The protein is part of the exocytotic fusion complex (v-SNARE) where it assembles with syntaxin1 and synaptobrevin.
SNAP23 is able to function in regulated exocytosis. Both isoforms may have their own specific binding partners and discrete, albeit mechanistically similar, functional roles within the cell.

1 ) Lin RC et al., Annu. Rev. Cell Dev. Biol. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Jahn R et al., Annu. Rev. Biochem. (1999)
4 ) Sadoul K et al., J. Biol. Chem. (1997)
5 ) Chen F et al., Biochemistry (1997)
6 ) Ravichandran V et al., J. Biol. Chem. (1996)
7 ) Hanson PI et al., J. Biol. Chem. (1995)
8 ) Südhof TC et al., Nature (1995)
9 ) Rothman JE et al., Nature (1994)

Protocols

Standard protocol

OTHER

Preabsorbtion

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